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D Anthony

Publications and source records attributed to D Anthony.

53 records · Page 3Linked to original sources
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Net know-how.

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Computer Communication Networks↗
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The French connection.

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Computer Communication Networks↗
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Networks for the rich?

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Computer Communication Networks↗
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Get ready for e-mail.

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Computer Communication Networks↗
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Avoiding the sharks.

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Computer User Training↗
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Nurses' knowledge about pressure sore treatment and healing.

Although nurses are more knowledgeable about pressure score healing and treatment than expected, a small scale study found that there is still room for improvement. There appeared to be similar levels of knowledge of pressure sore healing among orthopaedic and general surgical nurses, and a strong relationship between clinical grade and knowledge, with more senior grade nurses scoring higher on a pressure sore healing and treatment score, compared to more junior nurses. This has implications for who should be performing pressure sore treatment in the clinical setting.

Career Mobility↗
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Clinical guidelines in community hospitals.

AIM: The objective of this study was to investigate the attitudes of community hospital staff towards clinical guidelines. METHOD: Twenty four staff members from 11 hospitals in one community trust were interviewed. The participants, primarily F and G grade nurses, but also some physiotherapists and occupational therapists, were asked about their use of, attitudes to and sources of clinical guidelines. RESULTS: A simple gap analysis identified that participants were familiar with local clinical guidelines and generally felt positively about them, but had a few reservations. However, they were generally unaware of national guidelines. CONCLUSION: This study has identified the reality of implementing and raising awareness of clinical guidelines in practice.

Attitude of Health Personnel↗
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IGF-I triple helix strategy in hepatoma treatment.

BACKGROUND/AIMS: To investigate the effect of gene therapy for hepatocellular carcinoma based on inhibition of cellular IGF-I expression, the technique of IGF-I triple helix was investigated in mice developing programmed hepatoma. METHODOLOGY: mhAT1F1 mouse hepatoma cell line was transfected in vitro with IGF-I triple helix expression vector (pMT-AG-TH) or with IGF-I antisense expression vector (pMT-Anti-IGF-I). 10 x 10(6) transfected cells of either triple helix or antisense type were inoculated intraperitonealy into transgenic ATIIITB6 mice developing genetically programmed hepatoma (mice die between the age of 6 and 7 months). In parallel, human cell cultures established from surgically removed hepatomas were investigated. RESULTS: mhAT1F1 and human primary cell cultures, transfected with pMT-AG-TH or pMT-Anti-IGF-I vectors resulted in total inhibition of IGF-I demonstrated by immunocytochemical and Northern blot techniques. Transfected cells changed their phenotype and recovered major histocompatibility complex I expression showed by fluorescence-activated cell sorting analysis and Western blot. Moreover, two phenomena were observed in IGF-I "antisense" or "triple helix" transfected cells: 1) the apoptosis, demonstrated by TUNEL technique; 2) the presence of IL-6 simultaneously with disappearance of tumor necrosis factor-alpha and IL-10, investigated by reverse transcriptase-polymerase chain reaction technique. In in vivo experiments, injection of murine transfected cells into mice in terminal-phase prolonged their survival 3-4 months in 100% of cases, as well in "antisense" group (8/8) as in "triple helix" group (10/10). CONCLUSIONS: Injection of hepatoma cells transfected with IGF-I triple helix expression vector, and showing immunogenic and apoptotic characteristics, can constitute an effective cellular therapy against hepatocellular carcinoma.

Animals↗
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Yours in confidence.

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Computer Security↗
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Antisense insulin-like growth factor I transferred into a rat hepatoma cell line inhibits tumorigenesis by modulating major histocompatibility complex I cell surface expression.

We have established a hepatocarcinoma cell line (LFCI2 A) that produces voluminous tumors when injected subcutaneously into syngeneic Commentry rats. These neoplastic cells express both insulin-like growth factors (IGF) I and II. When transfected with an episomal cassette-expressing IGF-I antisense RNA, the modified LFCI2 A cell lines become poorly tumorigenic and, when injected subcutaneously, are associated with inhibition of the growth of the parental tumoral cells and/or induction of regression of established tumors. By contrast, cell lines isolated after transfection with the IGF-II antisense-expressing vector were as tumorigenic as the parental cell lines. The results are discussed in terms of protective immunity induced by the tumoral cells transfected by the IGF-I antisense vector. In the transfected hepatocarcinoma cells that do not produce IGF-I, the expression of major histocompatibility complex class I antigen was increased at least 4-fold compared with parental cells. The introduction of these cells in vivo induced a tumor-specific immunity that was associated with CD8 T cells.

Animals↗