Search PubMed⌕ Search

Biomedical subjects

C Wang

Publications and source records attributed to C Wang.

At least 1,207 records · Page 67Linked to original sources
PubMed1990

The effects of leukemia inhibitory factor (LIF) on the blast stem cells of acute myeloblastic leukemia.

Leukemia inhibitory factor (LIF) was tested using three established acute myelocytic leukemia (AML) cell lines. In growth assays in two of the three lines, we found that the addition of LIF increased the doubling time of the clonogenic population but not the total population as assessed by nucleated cell counts. Similarly, tritiated thymidine uptake into total AML populations was not affected by LIF, but the percentage of clonogenic cells killed by exposure to high specific activity 3HTdR was reduced in LIF-treated cultures compared to controls. We interpret these results to indicate that LIF prolongs the cell cycle of stem cells in some AML lines, possibly by increasing the time spent in the G2-M-G1 parts of the cycle. Consistent with this interpretation, we observed a decrease in ara-C sensitivity in LiF treated cultures. Variable results were obtained when freshly obtained AML blasts were exposed to LIF.

Cell Cycle↗
PubMed1990

[Involvement of oxytocin in spinal cord in acupuncture analgesia].

The influence of intrathecal injection of oxytocin (OT), anti-OT serum (AOTS) and naloxone on pain threshold and electroacupuncture (EA) analgesia in rats was investigated. The tail-flick induced by potassium iontophoresis was used to measure the pain threshold. The increase in pain threshold was observed within 70 min after OT injection (100 ng), and it was much more effective than that of the ACSF injection (P less than 0.001). The OT administration could enhance the EA analgesia. This effect was of dose-related. Although injection of AOTS did not affect the pain threshold, it diminished EA analgesia. Furthermore, injection of naloxone did not influence the action of OT on EA analgesia. Our results showed that OT in spinal cord plays an important role in the EA analgesia, and its effects is independent of endogenous opiate peptides.

Acupuncture Analgesia↗
PubMed1990

[Therapeutic effect of yupingfeng san and shengmai yin on Coxsackie B viral myocarditis].

A "LDH release method" was used to determine NK cell activity in 30 cases of Coxsackie B viral myocarditis and contrasted with 20 normal volunteers. About 80% cases revealed NK cell activity deficiency. Among them, the NK activity of 24 cases (80%) was less than 14%; and 15 cases (50%) less than 10%. After Yupingfeng San and Shengmai Yin treatment for 2-3 months, their NK cell activity was elevated from 12.26 +/- 1.31% to 31.99 +/- 4.23% (P less than 0.001). At the same time, their clinical manifestations and EKG changes were improved or returned to normal.

Adult↗
PubMed1990

[Relation of changes in plasma cAMP, cGMP and the clinical conditions, pathology and the type of traditional Chinese medicine in 50 cases of chronic severe icteric hepatitis].

Plasma of cAMP and cGMP in 30 cases of chronic active hepatitis (group of cholestatic patients) as well as in 20 cases of chronic hepatitis with submassive or massive necrosis (group of chronic severe hepatitis patients) were studied with 125I-marked radioimmunoassay. 50 cases with their serum bilirubin levels higher than 171 mumol/L were selected as subjects for this study. Among them, 42 were diagnosed as hepatitis B, 6 as coinfection of hepatitis A and B. According to differentiation of symptoms and signs of TCM, 24 were diagnosed as simple hepatitis due to blood stasis and blood heat, 17 as hepatitis due to blood stasis and blood heat accompanying symptoms of Yang deficiency of the Spleen and Kidney. 20 healthy persons were selected as controls. The results were as follows: cAMP was 56.82 +/- 25.54 ng/L and 80.32 +/- 20.73 ng/L, and cGMP was 9.07 +/- 6.56 ng/L and 19.49 +/- 9.34 ng/L in the group of cholestatic patients and in the group of chronic severe hepatitis patients respectively. Both were higher than those in the control group whose cAMP was 14.12 +/- 3.25 ng/L and cGMP was 5.87 +/- 1.44 ng/L (P less than 0.01). Increase in cGMP and decrease in the ratio of cAMP and cGMP in the cases accompanying symptoms of Yang deficiency of the Spleen and Kidney were much higher than those in the other two types of hepatitis patients (P less than 0.01 and 0.05).(ABSTRACT TRUNCATED AT 250 WORDS)

Adolescent↗
PubMed1990

[Ultrastructural study of the megakaryocytes in megaloblastic anemia].

The ultrastructure of the megakaryocytes in the bone marrow from 14 cases of megaloblastic anemia was studied. The commonest change observed was that the development of the nucleus lagged behind that of the cytoplasm. There were some evidences supporting the theory of ineffective thrombocytopoiesis. The mechanism of the relationship between the changes of morphology and function based on the impaired DNA synthesis and methylation due to folate and vitamin B12 deficiency were also discussed.

Anemia, Megaloblastic↗
PubMed1990

Long-term results following 131I treatment for Graves' disease in Hong Kong Chinese--discriminant factors predicting hypothyroidism.

The clinical outcome of 1028 Hong Kong Chinese patients with Graves' disease treated with radioiodine therapy and followed for a mean of 9.85 +/- 4.84 years (range 2-20) was analysed. Retreatment was required by 413 patients (40.2 per cent), with 134 patients (13.0 per cent) requiring more than two 131I doses. One hundred and eighty-nine patients received carbimazole after 131I until euthyroidism was achieved. The cumulative incidence of hypothyroidism at one, five, 10 and 15 years was 9.6 per cent, 31.4 per cent, 53.8 per cent and 65.8 per cent, respectively. The average incidence of hypothyroidism after the first two years was 3.3 per cent per annum. Stepwise logistic regression analysis of pretreatment variables suggested that a combination of adjunctive carbimazole therapy, absence of ophthalmopathy and longer effective half-lives of 131I were of value in predicting which patients were less likely to develop permanent hypothyroidism. However, the probability of accurately predicting permanent hypothyroidism based on the present model was only 60 per cent. We believe that no single pretreatment variable, or combination of variables, predicts long-term hypothyroidism with sufficient confidence to justify the use of a 'formula' approach for prescribing 131I therapy for Graves' disease.

Adolescent↗
PubMed1990

Genetic polymorphisms of HLA class III and GLO1 in Chinese Yao nationality.

Yao is one of the minority nationalities located mainly in the South of China (Guang Xi province). The purpose of this study was to examine HLA class III and GLO1 in Yao nationality to identify the difference between Yao and Han. Han is the major nationality in China. Yao has the lowest C2*C frequency (0.9336) in the world. Before this study the lowest C2*C frequency known was that of the Japanese (0.9386). Gene frequencies in Yao are the following: C2*C = 0.9336, C2*B = 0.0664; BF*S = 0.9071, BF*F = 0.0929; C4A*4 = 0.0253, C4A*3 = 0.6061, C4A*2 = 0.2121, C4A*1 = 0.0152, C4A*Q0 = 0.1414; C4B*3 = 0.0051, C4B*2 = 0.0657, C4B*1 = 0.7071, C4B*92 = 0.0455, C4B*95 = 0.0253, C4B*96 = 0.0253, C4B*12 = 0.0051, C4B*Q0 = 0.1212; GLO1*1 = 0.1161, GLO1*2 = 0.8839. We have compared these data with those reported for Han.

Blood Donors↗
PubMed1990

Evaluation of the semi-automated Autosperm semen analysis system. II. Comparison with conventional method, time-exposure photomicrography, and automated CellSoft system.

Semen analysis results obtained by a recently developed semi-automated Autosperm system (Amsaten N.V.S.A. Corp., De Pinte, Belgium) were compared with those obtained by the conventional, time-exposure photomicrographic, and automated CellSoft system (Cryo Resources Inc., New York, NY) analyses. The Autosperm system either over- or underestimated the sperm concentration in comparison with the conventional analysis and more often underestimated the sperm concentration in comparison with the automated CellSoft system analysis. Comparison of the results for percent sperm motility by the conventional and Autosperm analyses showed that the latter tended to underestimate the percentage of fast-swimming spermatozoa and overestimate the percentage of slow-swimming spermatozoa. There were considerable variations in the measurement of sperm movement characteristics between the Autosperm and time-exposure photomicrographic and automated CellSoft system analyses, respectively. These findings demonstrate that the performance of the Autosperm system does not agree well with those of the currently available methods employed in the present study. Part of the disagreement in measurements of sperm parameters could be because of the subjective elements inherent in the semi-automated Autosperm analysis.

Humans↗
PubMed1990

[An observation of the anticancer activity of mitoxantrone hydrochloride injection].

Experimental results of anticancer activity of DHAQ.HCl are reported in this paper. It was discovered that DHAQ.HCl, mitoxantrone hydrochloride, had an obvious anticancer effect on the experimental tumor strains:Ehrlich ascites cancer, Sarcoma-180, Leukemia P388, etc., and that it was more effective to Leukemia P388 than to other strains. The anticancer activity of DHAQ.HCl was better than DHAQ (P less than 0.05). No side effect was observed on the local sites (s.c.) of tumor planted or drug injected. And also a good anticancer effect was observed on the sites of tumor planted by local injection. It is suggested that this observation can provide some information on the choice of routes of administration of this drug clinically.

Animals↗
PubMed1990

[Observation of morphological genesis and histochemistry of pulmonary adenocarcinoma in mice].

In the developing process of pulmonary adenocarcinoma in mice induced by N-methyl-N'-nitro-N-nitrosoguanidine, the observations of histological pathology, ultrastructure and the changes of enzyme activity of the lesions related to pulmonary adenocarcinoma were made by light microscopy, transmission electron microscopy and enzyme histochemistry, by sacrificing animals at intervals. The results indicated that there was a series of precancerous lesions before the initiation of adenocarcinoma; that adenoma was a kind of precancerous lesion which might develop into adenocarcinoma; and that adenoma with malignant change might be a focus of early adenocarcinoma. Based on the results of histological pathology, ultrastructure and enzyme histochemistry, a process of the morphological genesis of pulmonary adenocarcinoma in mice was proposed, and the metabolic changes related to various lesions were discussed.

Adenocarcinoma↗
PubMed1990

Effect of three-year multidrug therapy in multibacillary leprosy patients.

The feasibility and effects of a 3-year treatment using rifampicin (RFP), clofazimine (B663) and dapsone (DDS) in multibacillary leprosy patients in Yangzhou Prefecture and Dongtai County (1983-1986) are reported. Among 591 active multibacillary leprosy patients in the two areas, 569 (96.30%) were treated with this regimen. Of 303 cases available for analysis, 196 (64.7%) cases showed negative skin smears and clinical inactivity. The rest showed different degrees of improvement. The average reduction of BI was 0.78. The intensity and frequency of ENL and neuritis decreased markedly with treatment. The main side-effects were pigmentation and ichthyosiform changes of the skin, but these did not influence treatment.

Adult↗
PubMed1990

[Relation of changes in plasma TXB2 PGF1alpha and differentiation of signs and symptoms in traditional Chinese medicine in severe icteric hepatitis].

Clinical observations and experimental studies were made in 52 cases of chronic cholestatic hepatitis, 30 cases of chronic severe hepatitis, 30 healthy control persons, and 30 animals with experimental intrahepatic cholestasis. The results were as follows: Plasma TXB2 and PGF1 alpha were higher than their normal values both in cases of chronic cholestatic hepatitis and in cases of chronic severe hepatitis (P less than 0.01), the ratio of TXB2 and PGF1 alpha was normal in cases of chronic severe hepatitis and lower than normal value in cases of chronic cholestatic hepatitis (P less than 0.01). The cases were divided into 4 types (hepatitis due to blood stasis and blood heat, blood stasis and blood heat accompanying symptoms of fluid retention in the epigastrium, damp-heat, and composite factors) according to differentiation of symptoms and signs on the basic theories of TCM. Significant difference was found in TXB2, PGF1 alpha, PGF1 alpha/TXB2 and bilirubin when compared with each other in the 4 types of hepatitis patients (P less than 0.01). Bilirubin, TXB2, PGF1 alpha and PGF1 alpha/TXB2 in the survivors became normal or close to normal after they were treated with blood-cooling and circulation-promoting Chinese herbal medicine and prescriptions by changing their dosages according to differentiation of symptoms and signs on the basic theories of TCM. The therapeutic effect of prescription II was more satisfactory than that of prescription I. Changes of plasma TXB2 and PGF1 alpha in the experimental animals were more significant than those both in the control group and in the group treated with prescriptions I and II (P less than 0.01), but in the liver only the ratio of TXB2 and PGF1 alpha was lower than that in normal animals (P less than 0.01).(ABSTRACT TRUNCATED AT 250 WORDS)

6-Ketoprostaglandin F1 alpha↗
PubMed1989

Predictive value of sperm morphology and movement characteristics in the outcome of in vitro fertilization of human oocytes.

One hundred fourteen semen samples from Chinese males were analyzed for routine semen parameters including the semen volume, sperm count, percentage motility, and percentage normal morphology. Of these 114 samples, 54 also had movement characteristics of seminal and swim-up sperm evaluated by the computer image analyzer system (Cellsoft; Cryo Resources Co., New York). All semen samples were subjected to the swim-up procedure to harvest the motile sperm before inseminations of human oocytes. Fertilization was considered to have occurred when at least one oocyte was observed with two or more pronuclei. Semen samples were classified as infertile (0% fertilization rate; N = 32) or fertile (greater than 0% fertilization rate; N = 82) before statistical analyses. There was a significant difference (P less than 0.005) in percentage normal morphology of seminal sperm between the fertile (mean +/- SE; 67.3 +/- 1.2%) and the infertile (59.3 +/- 2.2%) samples. The percentage normal morphology of seminal sperm correlated (r = 0.3049; P less than 0.002) with the fertilization rate and this parameter was selected by the multivariate stepwise discriminant analysis as the discriminator capable of predicting the fertilization rate with 57.9% accuracy. Statistical analyses of samples where sperm movement was also evaluated demonstrated that there was significant differences (P less than 0.01) between the fertile (N = 38) and the infertile (N = 16) samples in percentage normal morphology of seminal sperm (67.8 +/- 1.8% vs 56.2 +/- 2.6%) and curvilinear velocity of swim-up sperm (89.2 +/- 3.5 vs 68.2 +/- 7.2 microns/sec).(ABSTRACT TRUNCATED AT 250 WORDS)

Female↗