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Biomedical subjects

C Wang

Publications and source records attributed to C Wang.

At least 865 records · Page 48Linked to original sources
PubMed1996

[A preliminary study of the anti-cancer effect of tanshinone on hepatic carcinoma and its mechanism of action in mice].

In this paper is reported the study of the anti-cancer effect of Tanshinone and its mechanism of action in vivo. The proliferating kinetics of hepatic carcinoma H22 cells was measured in mice by in vivo bromodeoxyuridine (Brdu) labeling and immunohistochemical staining of the proliferating cell nuclear antigen (PCNA). The tumor inhibitory rates of Tanshinone were 50.0%, 38.5% and 40.6% in 3 experiments, respectively. Compared with those of the DMSO-treated control groups, the differences were statistically significant (P < 0.01). The Brdu labeling and PCNA positive cells were 51.8 +/- 7.9 and 451.1 +/- 26.1 respectively, which were significantly lower than those of the controls (84.4 +/- 24.3, 694.8 +/- 117.1) (P < 0.01). The results once more confirm that tanshinone has anti-cancer activity and suggest that the mechanism of action might be associated with inhibition of DNA synthesis, PCNA expression and DNA polymerase activity of tumor cells.

Abietanes↗
PubMed1996

[Influence of electroacupuncture and capsaicin treatment on AChE activity and [3H]-QNB binding sites in the spinal dorsal horn].

1.5% Capsaicin (Cap) or Vehicle was respectively used to treat the right or left sciatic nerve in 20 Sprague-Dawley rats. On the seventh day, the 20 rats were at random divided into electroacupuncture (EA) group and non-EA group, the spinal cord corresponding to the afferent segments of sciatic nerve was taken out for observing the changes of acetylcholinesterase (AChE) activity and [3H]-quinuelidinylbenzylate (QNB) binding sites in the spinal dorsal horn (SDH). The results were as follows: (1) EA "Huantiao" could enhance AChE activity in the SDH and decrease [3H]-QNB binding sites; (2) Cap treating sciatice nerve could weaken AChE activity in the SDH and merease [3H]-QNB binding sites; (3) Cap treatment could inhibit or partially inhibit the actions of EA as above. The results indicated that ACh participated in the primary afferent of acupuncture information and might exist in Cap-sensitive neurons.

Acetylcholinesterase↗
PubMed1996

[Segmental influence of dorsal root action potentials evoked by stimulating the acupoints after acupuncture along meridians].

Using the method of recording the corresponding dorsal root action potentials evoked by stimulating the acupoints, we found that electro-acupuncture could not only expand the segmental of potentials, but also amplitude the potentials. The results proved the acupuncture sensation may be transsegmental afferent after acupuncture along the meridians. This study gave a new evidence for the mechanism's explanation of propagated sensation along the meridians.

Acupuncture Points↗
PubMed1996

[Surgical treatment of unilateral alveolar cleft].

In this study, 16 cases of unilateral alveolar cleft with cleft lip and palate were repaired with autografts of cancellous bone (13 cases) or hydroxyapatite (3 cases). The grafts were covered by reflected mucoperiosteal flaps and a mucosal flap from the upper lip. Twelve of the thirteen cases were followed up for 1-5 years. Nine of whom using cancellous bone had bony continuity of the maxilla and 7 cases erupted permanent maxillary canines within the area of autografts. None of the 3 cases using hydroxyapatite erupted a canine tooth. The results showed that autograft was better than hydroxyapatite in terms of maxillary canine eruption.

Abnormalities, Multiple↗
PubMed1996

[The influence on radioulnar joints after single-bone fracture of the forearm in children].

For the sake of researching overgrowth after single-bone fracture of the forearm in children, 179 patients were reviewed, the range of follow-up was 5-8 years, mean 7.5 yrs. (Not including Montaggia's, Galeazzi fracture's and physeal injuries). Bilateral comparative X-ray assessments were as follows: length of ulna and radius, radioulnar angle, ulnar deviation angle and anteversion angle of the radius, radial capitular line, position of the humeral head, and the movement of wrist and elbow joint. Overgrowth of the fractured bones existed only in 4 patients' by the length of 2-4 mm. Movements of wrist and elbow joints were normal. As the forearm is a double-bone balanced extremity, the injury is not very severe after single-bone fracture and overgrowth is usually hard to be observed.

Adolescent↗
PubMed1996

[Repair of bilateral cleft lip with unilateral composite lip flap: report 19 patients].

To repair bilateral cleft lip seems to be more difficult than that of unilateral one. In order to correct insufficiency of fore-lip, we have applied the composite lip flap of the thicker side for bilateral cleft lip in 19 patients. Overall results were satisfactory. There was no peculiar appearance indicating that fore-lip is too long or tension in the postoperative follow up from six months to four and half years. In this article, the methods of rotating repair were described in detail. The advantage and key points of the teehnique were discussed. So it may be regarded as one of the ideal measures for repairing bilateral cleft lip.

Child, Preschool↗
PubMed1996

[Hypolipidemic action of soy fiber and its effects on platelet aggregation and coagulation time in rats].

In order to lay a scientific basis for the development and use of soy bean, six per cent of soy fiber (SF) and pure cellulose were added to normal feed containing 1% of cholesterol and 0.2% bile salt, to feed SD rats, as compared with a control group of rats fed only with the normal feed, to study the effects of fibers on blood lipid concentration, platelet aggregation and clotting time. Results showed SF could not only significantly lower serum total cholesterol (TC), LDL-C and atherosclerotic index, and increase the ratio of HDL-L/TC, but also significantly lower serum fibrinogen (FB) and platelet aggregation, prolong clotting time (P < 0.01). NO such effects were observed in rats fed with pure cellulose. The authors believed that hypolipidemic effect of SF was achieved by lowering LDL-C in animals with hyperlipemia, and lowering of blood lipid and FB were major reasons to make animals fed with SF-added feed lower their platelet aggregation and prolong their clotting time.

Animals↗
PubMed1996

[A preliminary study of the preventive and blocking effect of green tea grown in mengding mountain area on the esophageal cancer in rats].

Three groups (positive control or nitrosamine alone group, test group and control group) were included in this experiment. After the begining of the experiment, the rats of test group were given 2% green tea (common grade) solution by free drinking daily for 24 weeks. After 2 weeks of the begining of the experiment, the rats of the nitrosamine alone group and test group were injected with MANA, 5 mg/kg weekly for 20 weeks. The animals were sacrificed at 4 intervals. The incidence rates of esophageal cancer in the rats of the two groups sacrified at every interval were significantly different. The result suggested that green tea should have a preventive and blocking effect on the occurrence of esophageal cancer in rats.

Animals↗
PubMed1996

[Conservative treatment for early breast cancer].

68 Cases of early breast cancer were divided into local resection group (33 cases) and local resection plus axillary dissection group (35 cases). The 3-, 5- and 10-year survival rate of the two groups were 97.0%, 78.8%, 72.7% and 94.3%, 80.0%, 77.1% respectively; The metastasis rate of the two groups were 6.1%, 12.1%, 3.0% and 8.6%, 17.1%, 8.6% respectively; and the local recurrence rate of the two groups were 6.1%, 6.1%, 3.0%, and 5.7%, 5.7%, 2.9% respectively ( P > 0.05). The cosmetic satisfaction rate of the two groups were 60.6% and 65.7% respectively ( P > 0.05). Factors relating to cosmetic results were type of breast, operative incision, age and postoperative radiotherapy. Conservative operation for early breast cancer was safe, provided incision margin was 2 cm approximately 3 cm. In all patients mammplasty was performed after tumor resection, and contralateral breast were treated by mammominification when it was necessary.

Adenocarcinoma↗
PubMed1996

[Diagnostic significance of CT in the orbital inflammatory lesions].

OBJECTIVE: To analyze and summarize the CT signs of orbital inflammatory lesions. METHOD: HITACH-W, or EXEL 2400 pattern of CT was applied for the orbital scanning of 52 cases with orbital inflammatory lesions proved by clinicopathology. RESULTS: The CT signs of orbital inflammatory lesions in our series included exophthalmos, orbital mass, ill-marginated mass, multiple space involvement, extraocular muscle enlargement, scleral thickening, eyelid thickening, optic nerve thickening, orbital fat density increasing, lacrimal gland enlargement, sinusitis, subperiosteal abscess, ring-enhancement in masses, etc. CONCLUSION: All these findings on CT are of important significance for the diagnosis of orbital inflammatory lesions.

Adolescent↗
PubMed1996

[An experimental study on corneal nerve regeneration after penetrating keratoplasty with long-term cryopreserved rabbit corneas].

OBJECTIVE: To observe the morphology of rabbit corneal nerve regeneration in the grafts after penetrating keratoplasty (PKP) with long-term cryopreserved homogeneous donor material. METHODS: Both long-term cryopreserved and short-term moist-chamber preserved fresh rabbit corneas were applied in the keratoplasty. By means of glyoxylic-acid induced fluorescence (GIF) and acetylcholine esterase (AChE) staining histochemical techniques, the morphological features of adrenergic and cholinergic nerve regeneration were monitored under light microscope in those grafts from ten days to eight months after surgery. RESULTS: During various intervals, the morphological changes of neural reinnervation were similar in the two groups, and numerous subepithelial nerves that showed random changes in direction and caliber were apparent. The nerve endings in the epithelium were few. CONCLUSIONS: The cryopreservative procedures do not affect neural regeneration under light microscope. The depressed tactile sensitivity following the surgery is correlated with nerve endings in the epithelium subnormal in number and with the regeneration of corneal nerves abnormal in distribution and morphology.

Adrenergic Fibers↗
PubMed1996

[Observations on early stage intraocular pressure after modern extracapsular cataract extraction].

OBJECTIVE: To observe the early intraocular pressure (IOP) after extracapsular cataract extraction (ECCE). METHOD: ECCE was performed on 58 eyes which were randomly divided into 3 groups, and postoperatively, at various intervals the IOP was measured and compared. RESULTS: The low IOP was not measurable by tonometer at 1 hour after the operation. At postoperative 3 hours, the mean IOP was higher than that of the preoperative level. The peak of IOP elevation was between postoperative 6-12 hours, over 2.80 kPa (1 kPa = 7.5 mmHg) and > or = 4.0 kPa were in 31 eyes (53.45%) and 4 eyes (6.90%) respectively, and the highest IOP was 4.40 kPa. Naturally, the levels of IOP resumed normal in all cases in postoperative 24 hours. The IOPs in 5 eyes (8.62%) were lower than the normal level (< 1.33 kPa) within 72 hours after the operation. The postoperative IOP of the group with diamox administered preoperatively was lower than that of the group with either mannitol or without drugs (P < 0.05) to lower the IOP preoperatively. The eyes with normal postoperative IOP accounted for the majority. CONCLUSION: Preoperative oral administration of diamox is a measure to prevent the early elevation of IOP after ECCE.

Acetazolamide↗
PubMed1996

[Detection of multidrug resistance in patients with leukemia by using flow cytometry and RNA in situ hybridization].

Multidrug resistance (MDR) in leukemia and the reversal of MDR by cyclosporin A (CsA) in vitro have been studied through intracellular accumulation of daunorubicin (DNR) in leukemic myeloblasts. The study was carried out with real-time flow cytometry and relative expression levels of MDR, which is estimated by RNA in situ hybridization in 26 patients suffering from leukemia. The change of intracellular DNR accumulation in vitro after adding CsA was also analyzed. The results showed that intracellular DNR accumulation in newly diagnosed and treated patients (MDR1 negative) with remission increased significantly than that in refractory and relapsing patients (P < 0.01). Good reversal effect was obtained in refractory patients and MDR1 positive relapsing patients by adding CsA (P < 0.01). It is suggested that MDR detection by the two above-mentioned methods could help to project the chemotherapy schedule clinically, CsA had obvious reversal effect on the drug-resistant leukemic cells in vitro.

Adult↗
PubMed1996

[Gastric emptying and intragastric distribution of liquid and solid meal in patients with systemic sclerosis].

To evaluate the gastric emptying functions in patients with systemic sclerosis (SS), gastric emptying and intragastric distribution during emptying after a liquid-solid meal were studied by single photon emission computed tomography in 11 patients without gut symptoms and in 17 healthy volunteers. In patient group, half emptying times both of proximal (pT50) and of total stomach (T50) for both meal components were significantly longer than in controls. There were significant relationships respectively between the T50 and the pT50 for both liquid and solid meal. Out of 11 cases, 9 had delayed T50 of solid, which were accompanied by delayed emptying of liquid in 8 patients. The prolongation of gastric emptying of solid correlated significantly with the duration of the disease. However, the changes of the activity-time curves in the distal stomach during emptying were not reaching significant level when compared to the controls. In conclusion despite no existence of any gut symptoms our SS patients present significant delayed gastric emptying, which may be correlated to the dysfunction of tonic contraction of the proximal stomach attributed to autonomic neuropathy.

Adult↗
PubMed1996

[Consolidation therapy with high-dose cytarabine and daunorubicin for prolonged disease free survival in acute myelocytic leukemia].

In order to study the effect of consolidation therapy with high-dose cytarabine (Ara-C) and daunorubicin (DNR) for prolonged disease free survival (DFS) in acute myelocytic leukemia (AML), 51 patients with AML in remission were treated with this method. Ara-C at a dosage of 1.3g/m2 administered in 2-hour intravenous infusion every 12 hours for 10 doses. After the treatment of Ara-C, DNR was given at a daily dose of 45 mg/m2 in rapid intravenous infusion for two days. The median duration of complete remission (CR) was 32 (8.2-110) months. The duration of DFS was more than 3 years in 21 patients (41.18%) and more than 2 years in 35 (68.63%). In a control group, however, the median duration of CR was 10 months and the rates of patients with DFS more than 3 and 2 years were 12.5% and 21.8% respectively. It is shown that high-dose consolidation chemotherapy is an effective method to prolong DFS in patients with AML. Toxicities of high-dose Ara-C and DNR can be tolerated by patients with AML.

Adolescent↗
PubMed1996

[Immunogenicity of recombinant S. typhimurium ex. pressing a hybrid antigen of Plasmodium falciparum].

We have expressed a 74-peptide hybrid Plasmodium falciparum antigen as fusion protein in attenuated Salmonella typhimurium SL3261. Live organisms were orally immunized Rabbits with a dose of 2 x 10(9)cfu. Specific anti-serum were detected by ELISA after immunization. Obvious Delayed type hypersensitivity (DTH) could be induced by PfAg and GZ-C antigen. The recombinant vaccine had no evident side-effects to the hosts. Our studies indicate that attenuated Salmonella typhimurium SL3261 can express synthetic P. falciparum antigen with several epitopes and live organisms can activate special cell-mediated immunity and humoral immunity.

Animals↗