Biomedical subjects

C Wang

Publications and source records attributed to C Wang.

At least 559 records · Page 31Linked to original sources

PubMed1999

[Effect of diazepam on fetus after intravenous administration during the active phase in labour].

To investigate the effects of diazepam on fetal heart rate and Apgar scoring administered intravenously during the active phase in labour, sixty-two normal term parturients during the active phase of labour with the cervical dilation to 3-5 cm were randomly divided into two groups, diazepam group (n = 32) and control group (n = 30). In diazepam group, 10 mg diazepam was administered intravenously. The concentrations of diazepam in maternal and cord serum were measured by high performance liquid chromatography after birth and the fetal heart rates were monitored by electronic monitor before or after administration of diazepam and Apgar scores were determined immediately after birth. The results showed that the mean level of diazepam in umbilical cord serum was markedly higher than that of maternal serum. Fetal heart base line mutation in diazepam group was weaker than that of the control group. Apgar scores in the neonates of diazepam group was lower than those of the control group. The results indicate that the use of diazepam in pregnant women during active phase of labour may cause depression of fetus.

Adult↗

PubMed1999

[Analysis on the dietary lipid pattern in different areas of China].

Based on the "Total Diet Study" conducted in 1990, food samples from urban and rural inhabitants in the four regions of China(North I, North II, South I and South II) were analyzed separately. The results showed that, except the status in South II, the amount of fat and cholesterol intake existed obvious differences between urban and rural inhabitants in the same region. The dietary fat and cholesterol intake of rural inhabitants in North II region was obvious inadequate. Dietary fat intake of urban inhabitants was high and the cholesterol intake was higher than the level of 300 mg per capita. The results showed that the ratio of polyunsaturated fatty acids was relatively low but the ratio of monounsaturated fatty acids was relatively high for dietary fat in two southern regions. It might be beneficial to increase plant oil which contains high amount of polyunsaturated fatty acids for the inhabitants in southern regions. From these results, we concluded that the dietary lipids intake of Chinese inhabitants was generally appropriate. Considering the great difference in economic development and dietetic culture, the dietary suggestions should be different regions to regions.

Cholesterol↗

PubMed1999

[Electrocardiographic T-wave amplitude of normal population and its correlation with age, sex and race].

OBJECTIVE: To establish a normal electrocardiographic T wave amplitude standard in Chinese population according to age and sex. METHODS: The electrocardiographic T wave amplitudes in 4,322 persons among normal population; aged from birth to eighty-five years old divided into 11 age-groups, were analyzed. The 97.5th percentile(P97.5) and 2.5th percentile(P2.5) were considered as the upper and the lower limit of normal T wave amplitudes and T/R ratio. Wilcoxon rank sum test was used to determine whether the normal electrocardiographic T wave amplitude and the T/R ratio were different in P97.5 value versus mean + 1.96 s value, in men versus women, and in Chinese versus Americans. The linear correlation analysis was used to examine the association between normal T amplitude and age as well as between T/R ratio and age. RESULTS: The normal T wave amplitudes and T/R ratio in all leads were greater in P97.5 value than in mean + 1.96 s value and were greater in men than in women. The normal T wave amplitudes of adults were greater in Chinese than in Americans except V1 lead in which the amplitudes were greater in Americans than in Chinese. There was moderate or marked correlation of normal T wave amplitude and T/R ratio with age in most of the leads. CONCLUSION: There are marked differences of age, sex and race in normal electrocardiographic T wave amplitudes.

Adolescent↗

PubMed1999

[A new method for repair of huge incisional hernia of abdominal wall].

OBJECTIVE: To repair the huge incisional hernia of abdominal wall, a new surgical method was introduced. METHODS: Eight cases of huge ventral incisional hernia, developed in 3 months to 12 months after operation, were treated in this new method with the defects ranged from 8 cm x 4 cm to 12 cm x 6 cm. RESULTS: They were followed up for 6 months to 18 months after operation. The clinical results showed that all of the 8 cases recovered satisfactorily without recurrence. CONCLUSION: The new method was recommendable for its advantages of easier manipulation, shortened time, no tissue reaction and less tissue trauma from operation.

Abdominal Wall↗

PubMed1999

[Separation and identification of thellungianin G from the root of Pimpinella thellungiana Wolff].

OBJECTIVE: To study the effective components in the root of Pimpinella thellungiana METHOD: Column chromatography with silica gel and ODS was employed for the isolation and purification of ingredients. Their structures were elucidated by spectral analysis. RESULT: Three compounds were obtained and identified as 2-(1',2'-epoxy)-4-methoxy pheryl-2-methyl-butyrate(I),4-(1'-propenyl) phenol(II) and 2-methyl butyric acid(III). CONCLUSION: Compound I is a new compound and named thellungianin G. Compounds II and III were separated from P. Thellungiana for the first time.

Phenylbutyrates↗

PubMed1999

[Mesothelioma in rats following-intrapleural-injection of crocidolite].

In order to investigate the potential of pleural mesothelioma induced by crocidolite, author collected samples from four county areas (Dayao, Yaoan, Muding and Yanyuan) in China. A suspension of 1 ml containing 20 mg crocidolite fiber was injected into the right pleural cavity of each rat in various test groups and UICC crocidolite group, totally 40 mg. The negative control was given saline. The results showed that the incidence of mesothelioma was 56.0%-68.8% (in which the rate of induction in Yanyuan group was the highest), and the survival time of the first case of mesothelioma was 273-347 days in the test groups, the Yanyuan group had shorter latency, and the mean survival days of the rats with mesothelioma for four groups were 560, 490, 593 and 498 days respectively; the shortest mean survival time was that for Yanyuan group. The major histological type of mesothelioma induced by crocidolite was the fibrous type. The degree of differentiation in all mesotheliomas was mainly intermediate and low. The results suggested that when rats were intrapleurally inoculated with samples of crocidolite and saline, an appreciable proportion of animals developed mesothelioma except those of the saline group. Compared with other groups, the Yanyuan group had stronger potential of crocidolite-induced mesothelioma.

Animals↗

PubMed1999

[Experimental study on head and neck hemodynamics after ligation of jugular veins].

OBJECTIVE: To study alteration of neck hemodynamics after ligation of jugular veins during radical neck dissection. METHODS: 4 rhesus monkeys were selected to simulate the human bilateral radical neck dissection. After operation, the jugular veins were ligated or loosen and the blood stream quantities and blood pressure of jugular veins and arteries were measured simultaneously. RESULTS: The hemodynamic index would changed after jugular vein was ligated, the more the jugular veins were ligated, the greater effects the hemodynamics index of jugular veins were occurred, but there was little effect on arteries. CONCLUSION: The external jugular veins have the effect of compensating cerebral venous outflow after removing the internal jugular veins, and the external jugular veins should be conserved when bilateral or unilateral radical neck dissection was done.

Animals↗

PubMed1999

[Study of relative factors which influenced the embolic level of microspheres in the hamster lingual carcinoma].

OBJECTIVE: To study the relative factors which influence the embolic level of microspheres in the hamster lingual carcinoma. METHODS: Micropicture analysis instrument was used to measure the embolic level of microspheres which were poured through ascending aorta. RESULTS: The optimum pressure of embolization was 30 kPa. The embolic level approached to remote site of the lingual artery as tongue cancer developed. In early invasive cancer period, the embolic level of microspheres was at arteriole level where microspheres block "the total values" which controlled the tumor microcirculation. The optimum embolic result and less offshoot microcirculation were obtained at this time. When in later period, microspheres mainly embolized the meta-arteriole. As the microvessels dilated, the dose of microspheres must be increased to get the best embolic result. CONCLUSION: The experimental data for clinical choice of optimum pouring pressure, optimum opportunity and optimum cases.

Albumins↗

PubMed1999

[Diagnosis and treatment of central hemangioma of mandible].

OBJECTIVE: To investigate methods of diagnosis and treatment of hemangiomas of the mandible. METHODS: Nineteen cases of central hemangioma of the mandible treated in our department from 1971 to 1997 were studied retrospectively. Fourteen patients received surgical treatment in which ten patients had received complete curettage of the lesion and the other four had received part resection of the mandible. RESULTS: The most common signs and symptoms of this lesion were hypermobility of teeth and episodes of spontaneous hemorrhage from the surrounding gingival sulcus. Selective angiography was significant in the diagnosis of this lesion. Fourteen patients received surgical treatment and thirteen lesions were removed completely, except that one died of massive bleeding at the operation and DIC on the next day. CONCLUSION: Selective angiography is useful to diagnosis the central hemangioma of mandible, and the complete curettage of the hemangiomas in the body of mandible is the most appropriate operation for children. At the same time, ligature of the inferior alveolar artery can reduce the risk of bleeding during operation.

Adolescent↗

PubMed1999

[Morphologic characteristics of dental cuticle on teeth affected by diabetic periodontitis].

OBJECTIVE: To determine the nature of the dental cuticle found on teeth with diabetic periodontitis. METHODS: Thirteen permanent teeth which were extracted from three diabetic patients with periodontitis were studied, with the control group of 16 healthy teeth extracted for orthodontical reasons, then all the teeth were decalcified with 5% nitric acid, stained with HE, and examinated by using light microscope. RESULTS: A layer of thick coat, dental cuticle, covered the root surface of the teeth with diabetic periodontitis, and the cuticle which presented regular laminar structure extended from the cementoenamel junction to the apical portion with different thickness from 40 to 80 microns, but no such similar cuticle structure was found on the healthy teeth of the control group. CONCLUSION: There is abnormal cuticle structure on the teeth of diabetic patients with periodontitis.

Aged↗

PubMed1999

[Design of HA/Ti biomedical implants with the use of ion-beam-assisted deposition].

Hydroxy-apatite (Ca10(PO4)6(OH)2,HA) is a potential material for coating on metal implants owing to its bioaffinity and osteo-integration to body. At present, in addition to bulk HA directly used in restoration and reconstruction surgery, hydroxy-apatite is also coated on metal implants by plasma-spraying method, adhesion and sintering. However, these kinds of biomaterials are restricted in application due to their insufficient strength and weak bonding. It is of great advantage to modify the surface of titanium and its alloy by ion-beam-assisted deposition (IBAD) combining ion implantation with physical vapor deposition(PVD). This paper is focused on the design of HA/Ti implants by ion-beam-assisted deposition technique and the computation of fabricating parameter using Trim96 program. The present investigation lays a foundation of further researches and application.

Coated Materials, Biocompatible↗

PubMed1999

[Correlation of conformational change with enzyme activity of glucose isomerase in denaturants monitored with high performance liquid chromatography].

Glucose isomerase (GI) can catalyze in vitro the isomerization of D-glucose to D-fructose. So it is an extremely important industrial enzyme in the commercial conversion of starch to high fructose syrups. In the previous papers, we have purified and characterized the enzyme from streptomyces diastaticus M1033 of China and obtained the crystal structures by X-ray. In this paper, a method for measurement of the dynamic conformational change procedure of glucose isomerase in various concentrations of denaturants by HPLC has been established. At first the relative molecular mass of GI in solution is measured by HPLC on PROTEIN PAK 300SW (7.5 mm i.d. x 30 cm) column. The relative molecular mass of GI is about 150,000. So GI exists as tetramer in the solution without denaturants. In 0-5 mol/L guanidine hydrochloride, incubated at 30 degrees C for 30 min, GI is gradually dissociated into monomer, and at the same time its activity gradually disappears. In various concentrations of urea and incubation at 30 degrees C for 30 min (or 60 degrees C for 1 h), the results are different from that in guanidine, because the monomers peaks of GI is not found. Only in certain concentrations of urea, the small dimer peaks of GI is found, but the activity of GI significantly disappears. Moreover as the increase of the urea concentration, the retention time of tetramer peak is gradually decreases. From the fluorescence spectra, we found the conformation of GI changed in the solution of urea. So perhaps in urea, the conformation of GI become a little unfolded, and the active region is partly damaged, which makes GI partly inactive. Dissociation into inactive monomers and conformation partly unfolding are all the reason of GI inactivation in denaturants.

Aldose-Ketose Isomerases↗

PubMed1999

[Comparison of lignocellulolytic enzyme profiles secreted by Panus conchatus and Phanerochaete chrysosporium during solid state cultures].

Most of white-rot fungi, such as Phanerochaete chrysosporium, can cause severe concomitant cellulose degradation during biodegradation of lignocellulose. Panus conchatus, a white-rot fungus, can cause efficient delignification of straw with only limited concomitant cellulose degradation. The results in comparison of lignocellulolytic enzyme profiles secreted by P. conchatus and P. chrysosporium during solid state cultures have shown that laccase and Mn-dependent peroxidase are main lignin-degrading enzymes of these two fungi respectively; high activities of xylanase are secreted by both fungi; and much lower activities of cellulases i.e. endo-glucanase, avicelase and cellobiase, especially endo-glucanase, are produced by P. conchatus during the whole cultures. The results further confirm that Panus conchatus has ability of strong selective delignification of lignocellulose.

Biodegradation, Environmental↗

PubMed1999

[Breeding of yeast for beer manufacturing by inactivated protoplast fusion].

QSB-XI6, one of fusants, was obtained by PEG-induced fusion between UV-inactivated protoplasts of Saccharomyces cerevisiae LQ16 (Ile-, Datr) and heat-inactivated protoplasts of S. cerevisiae QSB7(Ala-, H2S-). The cell volume, bicmass, genotype and DNA content of the fusant were measured in comparison with of its parents and the results showed that QSB-XI6 was a fusant. The fermentation ability and the flocculent capacity of this fusant were higher than those of its parents. The diacetyl content of the beer produced by the fusant was measured and some other compositions of the beer was also analysed by gas chromatography. The sensory evaluation of the beer was much better and the flavour was distinctive. The results of many production trials in succession showed that all good characteristics of QSB-XI6 were stable.

Cell Fusion↗

PubMed1999

[The objective evaluation of hearing aids].

OBJECTIVE: To objectively evaluate the effect of hearing aids. METHOD: The procedure involved eliciting ABRs by clicks and slow vertex response (SVRs) by tone pips to the aided and unaided ear presented in sound field. RESULT AND CONCLUSION: There has high relativity between the thresholds of SVRs and free field pure tone audiometry. There has no significant difference between the gains from SVRs and free field pure tone audiometry.

Adolescent↗

PubMed1999

[Clinical use of nasal airflow measurement in septoplasty].

OBJECTIVE: To discuss the clinical use of nasal airflow measurement in septoplasty. METHOD: After nasal decongestion, 21 patients's nasal airway resistance and nasal airflow were evaluated by anterior rhinomanometry before and after submucous resection of nasal septum. RESULT: The amount of nasal airflow asymmetry could show nasal respiratory function better than nasal airway resistance does, the patients with absolute amount of nasal airflow asymmetry greater than 50% had bad postoperative outcome, patients' satisfication with operation was significantly correlated with the presence of post-operative symmetrical nasal airflow. CONCLUSION: The amount of nasal airflow asymmetry can serve as an indicator of patient selection, outcome evaluation and prognosis in nasal septum deviation.

Adult↗

PubMed1999

[Diagnosis and surgical treatment of brain-stem vascular malformations].

OBJECTIVE: To study the various aspects of the brain-stem vascular malformations, including the histopathology, clinical presentation, neuroimaging, techniques of surgical operation. METHODS: A retrospective analysis of 100 patients with 105 brain-stem vascular malformations had underwent microsurgery from 1987 to 1997 in the institute. RESULTS: Most brain-stem vascular malformations are cavernoma, some of them are not easily differentiated from telangiectasis. Meanwhile, they could also associate with venous malformation. In our group, 67% patients suffered from hemorrhage more than one time. The average number of bleeding in female is higher than in male's. There was no operative mortality. The outcome was improved and stable in 65% patients. Ninety-two patients have followed-up half a year to 11 years. Rebleeding after operation occurred in 4% cases. CONCLUSIONS: Active microsurgery should be adopted carefully for the patients with symptomatic brain-stem vascular malformations, while those with asymptomatic lesions need long-term clinical observations and neuroimaging follow-up.

Adolescent↗

PubMed1999

[Studies on the plant hormones produced by 5 species of endophytic fungi isolated from medicinal plants (Orchidacea)].

OBJECTIVE: To study the plant hormones produced by 5 species of endophytic fungi isolated from medicinal plants and to illustrate the mechanism on endophytic fungi stimulating the growth of plants. METHODS: Extracting plant hormones from mycelia and its culture solution with organic solvent, and detecting them by HPLC. RESULTS: One or more plant hormones [GA3 (Gibberellin), IAA (Indoleacetic acid), ABA (Abscisic acid), Z (Zeatin), ZR (Zeatin riboside)] were detected from the mycelia and its culture solution. CONCLUSIONS: The plant hormones produced by the endophytic fungi are important materials that may be used to reveal the mechanism of endophytic fungi stimulating the growth of medicinal plants (Orchidacea).

Abscisic Acid↗