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Biomedical subjects

C Su

Publications and source records attributed to C Su.

At least 91 records · Page 5Linked to original sources
PubMed1995

Mutagenic activity of dopamine after nitrosation.

Dopamine hydrochloride is reported to be a new mutagen precursor in this study. After treatment with nitrite under acidic conditions, dopamine hydrochloride showed direct-acting mutagenicity on Salmonella typhimurium TA100, TA98 and Escherichia coli WP2uvra. The addition of S9 mix did not affect the mutagenicity of nitrosated dopamine significantly in these three strains. Meanwhile, a comparison of the mutagenicity of nitrosated dopamine with nitrosated tyramine was carried out.

Dopamine↗
PubMed1995

[Amplifying Helicobacter pylori's urease gene by nested polymerase chain reaction].

By using nested polymerase chain reaction (PCR) composed of three primers that derived from Helicobacter pylori's (Hp) urease gene region, we have not only successfully amplified control Hp and 214 clinic samples but also studied deeply preparation of the sample, composition of PCR reaction liquid and circulatory mental conditions of the PCR. So this method shortens the operation time, simplifies processes and reduces costs. Compared with urease test (54%) and Warthin-Starry silver stained test (50%), ever used the positive rate of PCR is the highest (61%), so our method is more specific, sensitive, simple, rapid and accurate, and could be applied to the routine clinical detection.

Adolescent↗
PubMed1995

[Surgical treatment of familial acromegaly].

The annual incidence of the acromegaly is three cases per million, and that of the familial acromegaly is even far rarer. The number of familial acromegaly reported in the medical literature of all the world is fewer than 10 cases per year, and up till now none of such a patient has been reported in China. All the three cases of familial acromegaly reported here were from the same family, and showed typical clinical manifestations. They presented the history of acral enlargement, headache, amenorrhoea, elevated serum GH level, enlargement and erosion of pituitary fossa in X-ray and CT scan, and characteristic immunohistochemical findings of GH-secreting pituitary adenoma. All of them underwent successful transsphynoidal total removal of the pituitary adenoma, and recovered well by clinical, endocrine, and pathological criteria. An extended family tree of 65 members excluded consanguinous marriage and suggested that the mode of inheritance was irregular autosomal dominant.

Acromegaly↗
PubMed1995

[An investigation and design of apparatus for measuring fetal lung maturity].

Clinically, the authorized method for determing fetal lung maturity is the measurement of the ratio of surface-active L/S in amniotic fluid. To set up new, simple, rapid and accurate method, an apparatus for measuring fetal lung maturity was made. The paper describes the basic principle and working procedure of the apparatus. 148 amniotic fluid specimens from different pregnancies were measured by means of the apparatus. The result was compared with the L/S. The sensitivity of measurement was 95%, specificity 91%, and accuracy 93%. The operation of the apparatus is simple, convenient and rapid. A small quantity of amniotic fluid is needed. In conclusion, the expected result was attained. The apparatus is of great importance to clinical application.

Amniotic Fluid↗
PubMed1995

[Transsphenoidal microsurgical removal of GH-secreting pituitary adenoma in: a report of 200 cases].

From July 1979 to April 1992, 212 cases of GH-secreting pituitary adenoma were treated transsphenoidally by microsurgical technique, of whom two hundred cases monitored with their GH levels at preoperative and postoperative periods. The majority of cases totally 132 cases were of macroadenoma, and 68 cases were of microadenoma. 138 cases were operated on after April 1986. The cure and remission rate averaged to 72.5%, rising from 44.4% (before 1986) to 80.5% for microadenoma and from 31.4% to 69.1% for macroadenoma due to improvement of technique and accumulation of experience. One patient died of angiocardiopathy suddenly during operation. Mortality rate was 0.5%. The follow-up observation of postoperative GH levels in 52 cases revealed that the GH levels within 2 weeks after operation were the same as in 3-6 months. It is thought that the early GH levels may represent the surgical results. The surgical technique is described and factors influencing the surgical results, prevention and treatment of operative complications are discussed in this paper.

Adenoma, Acidophil↗
PubMed1994

Electrophoresis of proteins and protein-protein complexes in native polyacrylamide gels using a horizontal gel apparatus.

Electrophoresis of proteins and protein-protein complexes in polyacrylamide gels under native conditions using a horizontal gel apparatus is described. The advantage of this system is that it permits the detection of both negatively and positively charged proteins as well as protein-protein complexes in the same gel. During electrophoresis, a continuous gel sandwiched between two glass plates is placed horizontally on the platform and submerged in a reservoir buffer. The sample wells are made along the center of the gel, allowing positively and negatively charged proteins to migrate toward the cathode and anode, respectively. Several proteins with varying molecular weights and isoelectric point (pI) values and pairs of proteins capable of forming protein-protein complexes were chosen as model systems to illustrate the methodology. The effects of several parameters on the performance of the gel system including protein molecular weight, pI, and gel concentration were also examined and the results obtained by this method are comparable to those obtained by the vertical system. Following electrophoresis, both negatively and positively charged proteins as well as protein-protein complexes can be transferred by electroblotting onto polyvinylidene difluoride membranes for further analyses.

Electrophoresis, Polyacrylamide Gel↗
PubMed1994

Sterile ocular inflammatory reactions to monofilament suture material.

OBJECTIVE: To report six cases of sterile inflammatory reactions to fine monofilament suture materials, which is exceedingly rare in ophthalmology. METHODS: We report six patients (four underwent penetrating keratoplasty and two underwent cataract surgery) with unusually severe local inflammatory reactions to suture material. RESULTS: Patients developed multiple focal inflammatory infiltrates and corneal oedema related to 10/0 monofilament nylon sutures. Four patients had evidence of either atopy or raised serum IgE and one had an autoimmune disorder (systemic lupus erythematosis). Onset was four to 11 days in five cases and six weeks in one. Allograft reaction occurred in two of the four corneal grafts, and three grafts failed. One of the cataract patients developed significant against-the-rule astigmatism. Microbial cultures of corneal scrapings, donor corneal rims, and suture material were generally negative. Polymorphonuclear leucocytes were noted on Gram stain in four cases, and cytology of corneal scrapings demonstrated eosinophils in one case. CONCLUSIONS: Sterile inflammatory reactions to monofilament suture material are an uncommon complication of ocular surgery which may lead to corneal graft failure, and which is more common in atopic individuals. Systemic steroid therapy may be required.

Adolescent↗
PubMed1993

Electroblotting proteolytic products from native gel for direct N-terminal sequence analysis: an approach for studying protein-protein interaction.

Proteins which are electroblotted from native gels onto polyvinylidene difluoride (PVDF) membranes are suitable for detailed structural analysis. This method, in conjunction with limited proteolysis and N-terminal sequencing, has been used to study the molecular interactions between native protein molecules. The interaction between recombinant interleukin-2 (rIL-2) and its receptor (rIL-2R alpha) was examined as a model system. The working strategy consists of (i) proteolysis of rIL-2R alpha and rIL-2R alpha/rIL-2 complex, (ii) separation of the major proteolytic products by native polyacrylamide gel electrophoresis followed by electroblotting onto PVDF membrane, and (iii) sequence analysis of the blotted protein bands for the identification of peptide regions sensitive to proteolysis. Results have indicated that the exon 3 encoded region in rIL-2R alpha is sensitive to proteolysis regardless whether it is complexed with rIL-2 or not. This suggests that no major conformational changes occur in rIL-2R alpha during interaction with rIL-2. This electroblotting approach is, therefore, useful for studying protein-protein interaction in solution.

Animals↗
PubMed1993

Treatment and control of the intestinal fluke, Skrjabinotrema ovis.

The small intestinal fluke, Skrjabinotrema ovis, is common among sheep in the Xinjiang region of western China. Up to 65,000 flukes per sheep were found during September when sheep were grazing summer pastures at high altitudes. Efficacy studies of some common flukicides revealed that oxyclosanide and triclabendazole were not effective in reducing the egg output of S. ovis, whereas closantel and praziquantel reduced egg counts by up to 98%. Praziquantel at 25 and 50 mg kg-1 reduced worm counts by 99%. Albendazole at 15 mg kg-1 reduced egg counts by 83% and worm counts by 91% but at lower dose rates it was ineffective.

Albendazole↗