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Biomedical subjects

C Richard

Publications and source records attributed to C Richard.

431 records · Page 24Linked to original sources
PubMedDate not supplied

[Differentiation of "Pasteurella" and "Actinobacillus" from Enterobacteriaceae" by use of the O/129 vibriostatic agent (author's transl)].

Out of 134 Pasteurella and Actinobacillus strains studied, 132 are found to be susceptible to the O/129 vibriostatic agent with growth inhibition diameter ranging from 10 to 42 mm (86% in the range 25 to 32 mm); 46 reference strains of Enterobacteriaceae including Yersinia are resistant to the O/129 vibriostatic agent. This test can quickly differentiate between typical or atypical Enterobacteriaceae and the genus Pasteurella or Actinobacillus.

Actinobacillus↗
PubMedDate not supplied

[gamma-Glutamyl-transferase activity in the family "Neisseriaceae" (author's transl)].

Members of the family Neisseriaceae (554 strains) were screened for gamma-glutamyl-transferase (gamma GT) activity. gamma GT was produced by Neisseria meningitidis but not produced by any strain of N. gonorrhoeae, N. lactamica and Branhamella catarrhalis. Most non-proteolytic Acinetobacter calcoaceticus produced gamma GT whereas most A. lwoffii failed to produce gamma GT.

Acinetobacter↗
PubMedDate not supplied

[Calcinosis universalis associated with dermatomyositis. A report on five cases (author's transl)].

Five patients with dermatomyositis developed calcinosis universalis, the calcinosis lesions being diffuse in three cases and localized to a subcutaneous site in the other two. The diffuse lesions developed in young subjects with severe dermatomyositis, and increased progressively during recovery from the muscle disease. Treatment was ineffective. Subcutaneous calcifications can be detected at an early stage by technetium pyrophosphate scintigraphy, while the fluid nature of the swellings due to a local inflammatory reaction is proved by ultrasonography. Good results are obtained after excision of those calcified masses requiring removal because of their size, site, or superinfection.

Adolescent↗
PubMedDate not supplied

[Study of the species "Enterobacter gergoviae" by DNA/DNA hybridization (author's transl)].

A DNA/DNA hybridization study was carried out with 13 strains of Enterobacter gergoviae and 123 strains of Enterobacteriaceae, Mostly Enterobacter and Klebsiella species. E. gergoviae strains were very highly related (relative binding ratios: 88-96%) to the type strain CIP 76-01, as previously reported by Brenner and co-workers. No close genomic relationship was found with other species of Enterobacter and Klebsiella (mean relative binding ratio: 39%) and other Enterobacteriaceae.

DNA, Bacterial↗
PubMedDate not supplied

[Antineutrophil cytoplasmic antibodies in human pathologies].

The prevalence of antineutrophil cytoplasmic antibodies (ANCAs) was verified in 338 patients with various rheumatic diseases using the internationally standardized indirect immunofluorescence method. The method was improved by using ethidium bromide as a nuclear counterstain. We observed three patterns of fluorescence: the classical diffuse cytoplasmic granular staining (C); the perinuclear one (P) and a yet undescribed atypical cytoplasmic distribution (A). We thus found ANCAs in 14/15 (93%) patients with active Wegener's granulomatosis: 13 were C-ANCAs, 1 was P-ANCA. We also found ANCAs in 6/25 (22%) patients with other systemis vasculitides (essentially those with small vessel vasculitis): they were C-ANCAs (3 cases) or P-ANCAs (3 cases). Finally, ANCAs were detected in 10/185 patients with various autoimmune diseases (5 with rheumatoid arthritis and 5 with systemic lupus erythematosus): they were C-ANCAs (1 case), P-ANCAs (4 cases) or A-ANCAs (5 cases). No ANCAs were found in 100 control patients with miscellaneous diseases. The isotype distribution of ANCAs is essentially limited to IgG classes and the serum titre usually reflects disease activity. The technical improvement facilitates the distinction of ANCAs from other antinuclear and anticytoplasmic autoantibodies found in connective tissue diseases. Our data emphasize the clinical importance of ANCA detection and justify our efforts to develop more specific and quantitative assays.

Antibodies, Antineutrophil Cytoplasmic↗