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Biomedical subjects

C Mittermayer

Publications and source records attributed to C Mittermayer.

At least 163 records · Page 9Linked to original sources
PubMed1979

[Characteristics of human dental pulp cells in cell cultures].

The generation time and the division rate were measured with routine methods of cell culturing. The conditions contributing to the stagnation of growth were examined; the synchrony of the growth process was investigated. The biochemical composition of the cells was assessed via colorimetry and then compared with other cell systems. The cell culture examined may be used to test capping materials; only cells up through the eighth generation however should be utilized.

Adolescent↗
PubMed1978

Bleomycin: effect on satellite DNA in mouse fibroblasts.

The effect of Bleomycin on the semiconservative replication of mouse nuclear DNA has been studied. When asynchronously dividing mouse fibroblasts (L-cells) were grown in the presence of 5-bromodeoxyuridine (25 mg/l medium) for 18 h, three hybrid DNA bands with densities of 1.722, 1.752, and 1.761 kg/l appeared after caesium chloride density gradient centrifugation of nuclear DNA. In cells exposed to Bleomycin (100 mg/l) however, replication of satellite DNA is more strongly inhibited than is the replication of the main band DNA; preferentially the thymidinerich hybrid duplex at 1.761 kg/l could no longer be detected.

Animals↗
PubMed1978

The weight of human lungs as a diagnostic criterium (distinction of normal lungs from shock lungs by histologic, morphometric and biochemical investigations).

Lung weight, extravascular water content, hemoglobin content as an index of blood congestion, and DNA content as an index of cellular content, were determined for 57 patients dying from multiple, extrathoracic trauma. This report, which confirms previous publications, concerns itself in particular with the problems of determination of the "normal" lung weight. Studies of the influence of survival time on the lung showed that lung weight may significantly increase to twice its value after very short times up to one hour after trauma as a result of blood congestion, and following longer survival may be elevated several times as a result of developing interstitial edema. Early appearing interstitial edema is morphometrically detectable already 10 minutes after the beginning of shock. Normal weights were obtained from 10 cases after immediate death following severe cranial trauma or decapitation by being run over. Our results show that normal lungs have an average weight of 234 g (s +/- 39). Literature values for "normal" lung weight are approximately twice as high.

DNA↗
PubMed1978

The pulmonary air-blood barrier of human shock lungs (a clinical, ultrastructural and morphometric study).

Interstitial edema in the alveolar septa is the first morphologically recognisable change to be observed in cases of shock. It is brought about by the altered function of the membranes of the damaged epithelium and endothelium in the alveolar wall. At the same time there is an impairment of gaseous exchange, which is rendered more difficult by the exudative process in the interstitium. Pari passu with these events there is injury to the cells of both the alveolar epithelium and the alveolar capillary endothelium. Both these processes are still reversible. The point of irreversibility appears to be reached--so far as time is concerned--at the end of the first week, after which the injurious effects on the cell are established, since the thin alveolar wall necessary for the exchange of gases becomes overgrown with bulky alveocytes (Tpye II), and the fibroblasts in thealveolar interstitium push the capillaries away from the surface of the alveolus. In most of the advanced cases of shock this process of thickening of the alveolar wall exceeds the critical value, and respiratory exchange is so impaired that satisfactory functioning of the lungs is no longer possible.

Adult↗
PubMed1978

Is shock-induced lung fibrosis reversible? A report on recovery from "shock-lung".

As the consequence of a road accident a 19-year-old man developed respiratory insufficiency. Radiology and gas analysis revealed signs of "shock-lung". Morphometric analysis of a punch biopsy from the lung confirmed the diagnosis. Gas exchange grew worse in spite of conventional treatment. At the end of the third week after the trauma the maintenance of a highly negative fluid balance and the administration of high doses of corticoids were given a trial. The patient recovered. Although no functional pulmonary deficiencies remained, years later the patient still shows radiological evidence of extensive scarring. The lung parenchyma is intact, as judged by morphometric examination. This case shows that patients with "shock lung" can recover, but scars in the extrapulmonary space can remain, even though the lung parenchyma returns to normal.

Accidents, Traffic↗
PubMed1978

Standardized fixation of human lung for radiology and morphometry; Description of a "two chamber"-system with formaldehyde vapor inflation.

In order to obtain optimal preservation of lungs for histology, morphometry and radiology a two-chamber system of lung fixation with hot formaldehyde vapor is introduced. Compared with other procedures this system offers small dead space and ensures quick and thorough fixation by imitating "inspiration" and "expiration". Volume- and pressure-regulated "breathing" enables fixation in a stage of the respiration cycle, which can be chosen. This procedure can be used routinely in the autopsy room. The only serious drawback seems to be air pollution, if the chambers are not absolutely insulated.

Air Pollution↗
PubMed1978

[Aging in human gingival fibroblasts in cell culture. A contribution to the understanding of proliferating inflammations].

Proliferation of gingival fibroblasts was studied in cell cultures under controlled conditions. Biopsies taken from 12 patients of different ages were analyzed in regard to the length of the generation cycle and the ability to produce subcultures. Cultures from elderly individuals showed progressive, longer generation cycles than cultures from younger patients. The ability of the gingival cultures from elderly patients to produce subcultures is considerably less than those from young individuals. Apparently gingival fibroblasts are subject to aging processes in the patient himself and in the cell population within the cell culture. The growth characteristics differed depending on the location on the gingiva; the interdental papilla grow better cultures than do free and attached gingiva.

Aging↗
PubMed1978

[Shock lung].

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Humans↗