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Biomedical subjects

C Meyer

Publications and source records attributed to C Meyer.

At least 379 records · Page 21Linked to original sources
PubMed1992

[Value of a "floppy" Nissen in the treatment of gastroesophageal reflux. Apropos of 117 cases].

The Nissen fundoplication is the most widely used antireflux procedure. However this operation is associated with several specific complications. To prevent these complications, an alternative antireflux procedure ("floppy" Nissen) has been designed. This investigation was conducted to investigate the effects of the floppy Nissen performed on 117 patients, between 1978 and 1990. Indication for surgery was the endoscopic discovery of stage I and II oesophagitis (59 cases), III and IV oesophagitis (25 cases) or an associated disease (gallstones: 25 cases, duodenal ulcer: 8 cases), pH monitoring used preoperatively in 47 cases showed an important reflux in 33 cases (70%). Preoperative manometry exploration (71 cases) showed a low pressure of the low oesophageal sphincter (LOS) in 63 cases (89%). The "floppy" Nissen procedure was performed over a length 4 to 6 cm. The folds of the diaphragm were closed back behind the oesophagus in 40 cases. During the postoperative period the pH-data were restored to normal range in 73% (18/26 cases), and the value of the LOS was restored in 74% (19/26 cases). The mean duration of clinical follow-up study of 99 patients was 3.22 years. Improvement in clinical symptoms was noted for 86%; 13% had specific Nissen complications (9 gall-bloat syndrome, 4 intermittent dysphagia). Six patients had to be reoperated. This technique allowed good control of the reflux symptoms, with a low incidence of mechanical complications.

Adult↗
PubMed1992

Equipment nurses like.

Explore the source record for details and available documents.

Equipment and Supplies, Hospital↗
PubMed1992

[Lift mask with use of a malleable implant: BOP S.P. Initial results].

The mask lift, the latest technique for facial rejuvenation, comprises a large remodelling of the frontal and orbito-malar area. The modelling resections by bone-grinding are essentially designed to taise the superior orbital rim, but by the onlay grafts on the malar bone as well as the ones on the superior orbital rim many problems occur due to the donor site (autogenous bone), their biocompatibility and eventual resorption or integration. It is therefore proposed to use BOP S.P. as an onlay graft, a synthetic and biocompatible material already used in neurosurgery for cranioplasties with good results. Its essential advantage over other implants is its wax like consistency and its progressive adhesion to the bone, which allows onlay grafts to be placed wherever required; the modelling is fast, the result are immediately controllable and it is stable after a few hours hardening. BOP S.P. is essentially used as onlay graft material for mask lifts, but has also been successfully tested on 4 facial contour reconstructions for corrections after major traumatic injuries. No complication has been observed in a series of 9 cases but a one year follow-up is too short.

Biocompatible Materials↗
PubMed1991

Mutational and structural analysis of the nitrate reductase heme domain of Nicotiana plumbaginifolia.

We have analyzed four Nicotiana plumbaginifolia null mutants presumably affected in the heme domain of nitrate reductase. The DNA sequence of this domain has been determined for each mutant and for the wild type. Two mutations were identified as single base changes leading to, respectively, the substitution of a histidine residue by an asparagine (mutant E56) and to the appearance of an ochre stop codon (mutant E64). Based on the amino acid sequence homology between the nitrate reductase heme domain and mammalian cytochrome b5, we have predicted the three-dimensional structure of this domain. This showed that the nitrate reductase heme domain is structurally very similar to cytochrome b5 and it also confirmed that the residue involved in E56 mutation is one of the two heme-binding histidines. The two other mutations (mutants A1 and K21) were found to be, respectively, -1 and +1 frameshift mutations resulting in the appearance of an opal stop codon. These sequence data confirmed previous genetic and biochemical hypotheses on nitrate reductase-deficient mutants. Northern blot analysis of these mutants indicated that mutant E56 overexpressed the nitrate reductase mRNA, whereas the nonsense mutations present in the other mutants led to reduced levels of nitrate reductase mRNA.

Amino Acid Sequence↗
PubMed1991

Characteristics of Nicotiana tabacum nitrate reductase protein produced in Saccharomyces cerevisiae.

Tobacco nitrate reductase (NR) produced in yeast retains cytochrome c reductase activity, but not NR activity. Biochemical data suggest that the haem and FAD domains are functional, and that the molybdenum cofactor (MoCo) domain is inactive owing to the absence of MoCo in yeast. The native form of the produced NR is dimeric. Thus MoCo is not involved in NR dimerization in higher plants, contrary to current assumptions.

Blotting, Western↗
PubMed1991

The effects of five hematopoietic growth factors on human small cell lung carcinoma cell lines: interleukin 3 enhances the proliferation in one of the eleven cell lines.

Eleven small cell lung carcinoma cell lines of human origin were exposed to different colony stimulating factors (CSFs) to study whether CSFs could enhance the spontaneous cell proliferation and modify the action of cytotoxic drugs. In ten cell lines no suppressive or stimulative effect was observed when measured in a [3H]thymidine assay and a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. However, one cell line (GLC-20) could be stimulated by interleukin 3 (IL-3) when measured with a proliferative as well as a clonogenic assay. This enhancing effect was cell concentration dependent in the [3H]thymidine assay. Additional CSFs such as granulocyte-macrophage-CSF, granulocyte-CSF, IL-4, IL-6, insulin, or bombesin could not further augment the IL-3 supported proliferation. In addition, IL-3 binding studies demonstrated the presence of IL-3 receptors on the GLC-20 cells. Two types of receptors were demonstrated by Scatchard analysis: high affinity receptors (59 +/- 4 sites/cell) with a dissociation constant (Kd) of 31 +/- 9 pmol/liter; and low affinity receptors (1915 +/- 91 sites/cell) with a Kd of 2.0 +/- 0.8 nmol/liter. Finally, it was shown that the toxic effects of adriamycin and cisplatin on the proliferation of the GLC-20 cell line could partially be abrogated in the presence of IL-3. These data indicate that in some cases CSFs can modulate the proliferation of small cell lung carcinoma cell lines and interfere with the effects of chemotherapeutic drugs.

Bombesin↗
PubMed1991

1H spectroscopic imaging using a spectral-spatial excitation pulse.

Excellent water suppression is required to perform in vivo 1H spectroscopic experiments. However water suppression is difficult due to both B0 and RF inhomogeneities. These inhomogeneities are particularly troublesome in spectroscopic imaging experiments where water suppression is required throughout some large region of interest. In this paper, we propose the use of spectral-spatial excitation pulses for such experiments. These two-dimensional pulses are shown to provide water suppression that is insensitive to a range of B0 and RF variations while simultaneously providing spatial localization. Experimental results including images (with voxel volumes ranging from 3.4 to 1.5 cc) of various brain metabolites from both a normal volunteer and a patient with a metastatic lung carcinoma are presented.

Brain↗
PubMed1991

Effects of Tnt1 tobacco retrotransposon insertion on target gene transcription.

The effects of Tnt1 retrotransposon insertion on nitrate reductase (NR) gene transcription have been analyzed in three NR-deficient insertional, mutants of Nicotiana tabacum. In the three mutants, named h9-Nia4, h9-Nia5 and h9-Nia6, Tnt1 was inserted into exon 3, exon 2 and exon 1 of the nia2 NR alloallelle, respectively. The mutants h9-Nia4 and h9-Nia6, which contained Tnt1 insertions that were oriented opposite to the direction of nia2 gene transcription, expressed chimaeric nia2-Tnt1 RNAs, respectively 12 kb and 10 kb long. The size observed in h9-Nia6 was close to the expected size for a full-length hybrid transcript starting and ending under the control of nia2 signals (about 9 kb). The larger transcript found in h9-Nia4 was shown to be due to a failure to splice the nia2 intron 2. The mutant h9-Nia5, which contained a Tnt1 insertion oriented in parallel with the direction of nia2 transcription expressed two truncated nia2-Tnt1 RNAs, 2 kb and 6.7 kb long. These transcripts arose from termination in the long terminal repeats (LTRs) of Tnt1. Since no full-length hybrid RNA was detected, we suggest that Tnt1 carries efficient termination signals, which are more efficiently recognized in the 3' LTR than in the 5' LTR.

Base Sequence↗