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Biomedical subjects

C M Habibullah

Publications and source records attributed to C M Habibullah.

At least 37 records · Page 2Linked to original sources
PubMed1994

Leucocyte migration inhibition index responses of patients of amoebic liver abscess to various antigens of Entamoeba histolytica.

The whole amoebic lysate (WAL), Sephadex G-200 eluted fraction-I. (F-I), detergent dissected membrane proteins (DDMP), amoebic membrane glycoprotein (AMG) and amoebal RNA were extracted from E. histolytica. Amoebal RNA recognized 98.21% where as F-I, AMG, DDMP and WAL recognised 92.85, 91.07, 89.28 and 75% of total 56 clinically proven and amoebic serology positive amoebic liver abscess cases respectively. Intensity of recognition of individual fraction i.e., leucocyte migration inhibition index of each fraction was found to be highly significant (P < 0.001) when compared to whole amoebic lysate. This indicate the leucocyte migration inhibition index responses of patients of amoebic liver abscess to various antigenic fractions of E. histolytica with variations.

Adult↗
PubMed1993

Antibody-induced alterations of Na+/K(+)-ATPase activity in rabbit red blood cells during experimental thyroglobulin immunization.

Thyroid hormone modulates cell membrane Na+/K(+)-ATPase. A detailed study of erythrocyte membrane Na+/K(+)-ATPase activity was carried out during experimental thyroglobulin immunization. During the process of immunization there was a sharp decline in Na+/K(+)-ATPase activity until the 98th day of immunization, when the thyroglobulin antibody titer was at its peak. This enzyme activity retained a lower level although a fall in antibody titer occurred up to the 147th day.

Animals↗
PubMed1993

Decreased adenosine deaminase activity in peripheral lymphocytes of patients suffering from amebic liver abscess.

Amebic liver abscess (ALA) is associated with a state of transient suppression of cell-mediated immunity (CMI). T4 helper-inducer cell number is reported to be reduced. However, little is known of the reason for such reduction. Adenosine deaminase (ADA), an enzyme of salvage pathway in purine metabolism, is reported to be essential for the normal growth, differentiation and proliferation of T-lymphoid cells. In a pilot study we estimated the ADA levels in the peripheral lymphocytes of ALA patients. It was observed that the mean enzyme level in patients was 223.98 +/- 51.81 as against 405 +/- 38.12 in controls. The significantly low levels (p < 0.05) of enzyme in patients' lymphocytes may possibly explain the reduction of T4 helper-inducer cells reported in these cases. This parameter, if further evaluated, can serve as a differentiation marker between helper and suppressor T-cell subsets.

Adenosine Deaminase↗
PubMed1993

Detection of antibodies to serum IgA and IgG in peptic ulcer cases by indirect haemagglutination test.

Autoantibodies to serum IgA and IgG were detected in 206 peptic ulcer patients (196 duodenal ulcer and 10 gastric ulcer) and 52 healthy age and sex-matched controls by indirect haemagglutination test. A significantly increased mean titre of autoantibodies to serum IgA was observed in the peptic ulcer patients. Forty-eight (24.61%) duodenal ulcer cases had autoantibody titre values above mean +/- 2 SD of controls. Titres of autoantibodies to serum IgG were significantly increased only in the gastric ulcer cases. The results suggest an immunologic abnormality in a sizeable fraction of these cases.

Antibodies, Anti-Idiotypic↗
PubMed1993

Correlation between membrane glycoprotein and detergent dissected membrane protein in the assessment of LMIF: a report of 51 ALA cases.

Assessment of 51 amoebic liver abscess cases for leukocyte migration inhibition factor released using membrane glycoprotein and detergent dissected membrane protein (DDMP) of axenic Entamoeba histolytica (NIH:200). Lymphokines release by T lymphocytes in response to purified amoebic membrane glycoprotein (PAMG) against whole amoebic lysate (WAL), dissect out protein against whole amoebic lysate and membrane glycoprotein against dissected protein was tested by leukocyte migration inhibition test on blood samples from proved amoebic liver abscess cases. A significant increase was noted in the release of lymphokines and 100% positivity was observed with both PAMG and DDMP compared to 78% with whole amoebic lysate. The difference between means leukocyte migration indices of the membrane glycoprotein and whole amoebic lysate, detergent dissected protein and whole amoebic lysate with regards to release LMIF were found to be highly significant (P < 0.001), (P < 0.005) respectively. But insignificant difference and very much similarity was noted between the means of membrane glycoprotein and dissect out protein sensitized T lymphocytes with regards to lymphokine release in vitro. This shows the patients had high degree of leukocyte sensitized to pure amoebic membrane glycoprotein and detergent dissected membrane protein compared to whole amoebic lysate. These findings indicate that detergent dissected protein has similar antigenicity with membrane glycoprotein in elicitation cell mediated immune response in amoebic liver abscess cases.

Animals↗
PubMed1993

Correlation between antiamoebic IgG and autoreactive anti-IgG in amoebic liver abscess cases.

IgG isolated and purified from a healthy human serum through Sephadex G-200 and protein A CL 4B sepharose chromatography was used for detection of its own antibodies and correlated with the antiamoebic antibody titres in amoebic liver abscess cases. The mean titres with standard deviation of the self reactive antibodies to serum IgG both ALA cases and healthy controls show a highly significant difference, and antiamoebic antibody titres (IgG) are very much correlated with the autoreactive anti IgG titres in amoebic liver abscess cases. This correlation suggests that as antiamoebic IgG levels reach to its maximum, autoreactive anti IgG are produced to switch off antiamoebic anti IgG production in amoebic liver abscess cases.

Animals↗
PubMed1993

Detection of sialic acid in trophozoites of Entamoeba histolytica (NIH:200).

In two successive experiments detection of sialic acid was achieved from trophozoites of axenically grown E. histolytica (NIH:200) using silicic acid column chromatography for the separation of sialosylated lipids and asialosylated lipids from total lipids. Sialic acid of the sialosylated lipids was detected through thiobarbituric acid assay followed by acid hydrolysis. These findings indicate that presence of sialic acid is not only a character of the cystic stage but also that of trophozoites of E. histolytica. Detection of sialic acid confirmed the electronegative charge of the surface membrane (glycocalyx) of E. histolytica trophozoites.

Animals↗
PubMed1993

Isoenzyme pattern of clones of Entamoeba histolytica.

The isoenzyme pattern of clones of E. histolytica isolated from symptomatic and asymptomatic patients of amoebiasis were determined by starch gel electrophoresis. Ten parent cultures (uncloned), 5 from symptomatic and 5 from asymptomatic patients were isolated and established in Robinsons medium as xenic cultures. A total of 100 clones were isolated from parent cultures but only 65 could be established. Starch gel electrophoresis was carried out on the uncloned and cloned cultures. The analysis showed that the 5 uncloned culture from symptomatic patients had 4 isolates with pathogenic zymodeme and 1 isolate with non-pathogenic zymodeme pattern. The presence of beta band and absence of alpha band for phosphoglucomutase (PGM) and a fast moving Hexokinase (HK) band were taken as markers of pathogenicity. The 5 cloned cultures from asymptomatic patients had three isolates with pathogenic and 2 with non-pathogenic pattern. The cloned isolates from symptomatic patients (35) had 20 pathogenic zymodemes and asymptomatic patients (30) had 15 pathogenic zymodemes. Non-pathogenic zymodeme obtained in the present study were I, IV, V, X and XIII and the pathogenic zymodemes obtained were II and XIV. The data of the study indicated that (i) asymptomatic patients also harbour pathogenic zymodemes; and (ii) zymodeme II also coexsist with zymodeme XIV in the Indian population.

Animals↗
PubMed1992

Enteric non-A, non-B hepatitis: epidemics, animal transmission, and hepatitis E virus detection by the polymerase chain reaction.

We studied epidemics of viral hepatitis occurring at three different places in India. One was a combined epidemic due to hepatitis E virus (HEV) and hepatitis A virus (HAV) infections. In this epidemic, HAV affected children below 10 years of age, whereas HEV infected the young adult population. HEV was transmitted to rhesus monkeys (Macaca mulata) and confirmed by the polymerase chain reaction (PCR) on bile from the animals. Fecal material from acutely infected patients in one of the epidemics was also found positive for HEV RNA by PCR. This may help in confirming the nature of future epidemics. The bile and liver from experimental animals can be used as a source of material for further virological and molecular biological studies of HEV.

Adolescent↗
PubMed1992

Studies on urea cycle enzyme levels in the human fetal liver at different gestational ages.

Urea cycle enzymes involved in the detoxification of ammonia were studied in liver tissues of 57 male and 49 female fetuses of different age groups ranging from 13 to 36 wk of gestation. Surgical wedge biopsies of liver from 18 male and 12 female adults were used as controls. Significant enzyme activity was found to be present as early as the 13th wk of gestation. As gestational age advanced, enzyme activity gradually increased, reaching about 90% of the adult activity by the 36th wk of gestation.

Adult↗
PubMed1992

Autoimmunity in amoebiasis.

Amoebic liver abscess (ALA) and symptomatic intestinal amoebiasis cases were assessed by indirect haemagglutination assay for auto-reactive IgG and IgA class of antibodies in response to healthy human serum IgG and IgA. The present results indicated the presence of autoreactive IgG and IgA class of antibodies in ALA and intestinal amoebiasis respectively.

Autoimmunity↗
PubMed1992

Role of pure and biologically active amoebal RNA in assessment of lymphokines: a report of 55 ALA cases.

Amoebic liver abscess cases (55) were assessed for release of lymphokines (LMIF) using pure and biologically active amoebal RNA of axenic Entamoeba histolytica (NIH: 200) obtained with cesium chloride centrifugation. Lymphokines released by T lymphocytes in response to both amoebal RNA and whole amoebic lysate (WAL) were tested by leukocyte migration inhibition test (LMIT) on blood samples from amoebic liver abscess cases. A significant increase was observed in the release of lymphokine and 100% positivity was observed with amoebal RNA compared to whole amoebic extract with a positivity of only 78%. The difference between means leukocyte migration inhibition of the above two with regards to release of lymphokine was highly significant (P less than 0.001). This shows that patients had high degree of leukocyte sensitization to amoebal RNA of E. histolytica compared to whole amoebic lysate. These findings suggest that the amoebal RNA plays an important role as a potent antigen in the elicitation of cell mediated immune responses in amoebic liver abscess cases.

Adult↗
PubMed1992

Detection of autoreactive antibodies to serum IgG and IgA in amoebic liver abscess cases.

Assessment of autoreactive antibodies in response to healthy human serum IgA and IgG was performed by indirect haemagglutination assay on serum samples from 81 amoebic liver abscess cases for IgA and 70 for IgG. Appropriate controls were taken simultaneously. IgA, IgG were isolated and purified from a healthy human serum through Sephadex G-200 and protein A CL 4B sepharose chromatography. These immunoglobulins were used for the detection of its own antibodies in amoebic liver abscess cases. This revealed that 43.20% and 48.50% of the cases were positive for IgA and IgG respectively, where as only 19.35% and 28.30% of the controls were in positive category (IgA and IgG respectively). The mean titres with standard deviation of the autoreactive antibodies to serum IgA both in ALA cases and controls shows a highly significant difference between tests and controls (P less than 0.001). Similarly the mean titres with standard deviation both in ALA and controls for the serum IgG differed significantly (P less than 0.001). This suggests the presence of autoreactive antibodies against serum IgA and IgG in amoebic liver abscess cases.

Antibodies, Anti-Idiotypic↗