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Biomedical subjects

C M Chapman

Publications and source records attributed to C M Chapman.

At least 19 recordsLinked to original sources
PubMed2001

Polymorphisms in the angiotensinogen gene are associated with carotid intimal-medial thickening in females from a community-based population.

BACKGROUND: Polymorphisms within genes of the renin-angiotensin system have been associated with an increased risk of cardiovascular disease. We investigated the association of polymorphisms in the angiotensinogen (AGT) and angiotensin II receptor type 1 (AGTR1) genes with increased intima-media thickness (IMT) and the presence of plaques in carotid arteries. METHODS: Subjects (1111) from the Perth Carotid Ultrasound Disease Assessment Study (CUDAS) were genotyped for three polymorphisms: two in the promoter of the AGT gene, G-6A and A-20C; and one in the AGTR1 gene, A1166C. RESULTS: Using multivariate generalised linear models, the AGT-6A allele (P<0.001) and the AGT-20C allele (P<0.03) were significantly associated with increased mean carotid IMT in females but not in males when adjusted for conventional risk factors. The AGTR1 A1166C polymorphism did not show any significant relationship to mean IMT. Results suggest that the I allele of the angiotensin converting enzyme insertion/deletion polymorphism may interact with the AGT-6G allele to increase mean carotid IMT in the population as a whole. None of the polymorphisms investigated were significantly associated with the presence of carotid plaques. CONCLUSION: This study shows that polymorphisms in the angiotensinogen gene are associated with an increased risk of carotid intimal-medial wall thickening in females.

Adult↗
PubMed2001

A Pro12Ala polymorphism in the human peroxisome proliferator-activated receptor-gamma 2 is associated with combined hyperlipidaemia in obesity.

OBJECTIVE: Peroxisome proliferator-activated receptor-gamma 2 (PPAR gamma 2) is an important regulator of adipose tissue metabolism and insulin sensitivity. The aim of this investigation was to determine whether a PPAR gamma 2 Pro12Ala polymorphism was associated with cardiovascular risk factors (obesity, blood pressure, diabetes and blood lipids) in Western Australian Caucasians (n=663). DESIGN: Subjects were selected from two population studies (the Carotid Ultrasound Disease Assessment Study (CUDAS) and Busselton Population Health Survey) on the basis of body mass index (BMI). 292 obese (BMI > or =30 kg/m) and 371 lean (BMI <25 kg /m) subjects were studied. METHODS: Blood pressure and anthropometric measurements were collected from all participants, as well as a fasting venous blood sample. Biochemical measurements (high-density lipoprotein (HDL)- and low-density lipoprotein-cholesterol, triglycerides) and PPAR gamma 2 Pro12Ala genotype were also determined. RESULTS: Obese Pro/Ala and Ala/Ala subjects had lower levels of HDL-cholesterol (P=0.032) and a trend towards higher levels of triglycerides (P=0.055) compared with obese Pro/Pro subjects. In the obese group, the Ala allele was significantly associated with the presence of combined hyperlipidaemia (odds ratio = 2.33, P=0.042). There was no significant difference in the frequency of the polymorphism between lean and obese groups (P=0.069). No association was observed between Pro12Ala genotype and obesity, blood pressure or diabetes in either group. CONCLUSIONS: Obese carriers of the Pro12Ala polymorphism have a greater risk of developing combined hyperlipidaemia, possibly due to impaired activation of PPAR gamma target genes. The Pro12Ala polymorphism is not directly associated with obesity, hypertension or diabetes in this population.

Aging↗
PubMed1999

A high-throughput MS-PCR method on MADGE gels for ANG II type-1 receptor A1166C polymorphism.

We have developed a highly accurate, low-cost, single-step, mutagenically separated polymerase chain reaction (MS-PCR) method for the determination of angiotensin II type-1 receptor (AT(1)) A1166C gene polymorphism. The genotypes are determined using the microtiter array diagonal gel electrophoresis (MADGE) system. We have compared the MS-PCR method with allele-specific oligonucleotide hybridization and Dde I digestion techniques for determining the AT(1) A1166C genotype. The combination of MS-PCR and MADGE serves as a model for high-throughput single-nucleotide polymorphism genotyping in large population studies.

Alleles↗
PubMed1998

The different alternatively spliced isoforms of the Oct-2 transcription factor repress the involucrin promoter in a cell type-specific manner.

It has previously been reported that several octamer binding transcription factors including the Oct-2 factor can repress the involucrin gene promoter in keratinocyte cells. As the Oct-2 factor exists in several different cell type-specific isoforms with distinct activating or inhibiting effects on gene expression, we have tested the effect of these forms on the involucrin promoter. We report here that at high concentrations each of these isoforms can inhibit the involucrin promoter in keratinocytes. In other cell types however, all three isoforms activate the involucrin promoter and this effect is also observed at low concentrations of Oct-2 in keratinocytes. The mechanisms responsible for these effects are discussed.

Alternative Splicing↗
PubMed1997

Determination of kinetic and retention properties of cartridge and disk devices for solid-phase extraction.

The kinetic properties of cartridge and disk solid-phase extraction devices are determined by forced-flow liquid chromatography. Typical cartridges provide about 5-15 theoretical plates per cm of bed height and particle-loaded membranes provide about 4-9 theoretical plates for a 0.5-mm-thick membrane. It is shown that cartridge devices fail to provide their maximum trapping performance because of inadequate packing density and that the required packing density could be easily achieved in practice with particles of a standard size. The retention properties of common sorbents for extraction from water and air are characterized with the solvation parameter model. For predominantly aqueous solutions a favorable cavity term results in increased retention while polar interactions tend to reduce retention. Retention on porous polymer sorbents is more complicated because of their capacity to absorb significant amounts of the sample processing solvent resulting in solvent-dependent changes in retention properties. For trapping organic volatiles from air cavity formation and dispersion interactions are important, and in the case of Tenax its capacity for induction interactions is also significant.

Adsorption↗
PubMed1994

Olsalazine in ankylosing spondylitis: a pilot study.

OBJECTIVE: To test the safety and efficacy of olsalazine in men with ankylosing spondylitis (AS) unresponsive to nonsteroidal antiinflammatory drugs and physiotherapy. METHODS: Four patients, including 2 who had not responded to sulfasalazine (SASP) and one who did not tolerate SASP, were treated with olsalazine, up to 3 g/dl for 24 weeks. RESULTS: One patient discontinued olsalazine due to diarrhea at 1 g/day. The other 3 experienced improvement in global spine self assessment by visual analog scale (VAS), 2 of 3 patients, spinal pain (VAS, 3 of 3), night pain (3 of 3), tender joint count (3 of 3) and enthesis score (3 of 3). Changes in Schober's test and chest expansion were minor. Erythrocyte sedimentation rate and C-reactive protein were normal. Loose stools were the only adverse effects observed. CONCLUSION: Olsalazine appears to be well tolerated and effective in men with AS. Further study of olsalazine and direct comparison with SASP in this population may illuminate mechanisms of drug action and add a new therapeutic option.

Adolescent↗
PubMed1992

ATLAS-plus: multimedia instruction in embryology, gross anatomy, and histology.

ATLAS-plus [Advanced Tools for Learning Anatomical Structure] is a multimedia program used to assist in the teaching of anatomy at the University of Michigan Medical School. ATLAS-plus contains three courses: Histology, Embryology, and Gross Anatomy. In addition to the three courses, a glossary containing terms from the three courses is available. All three courses and the glossary are accessible in the ATLAS-plus environment. The ATLAS-plus environment provides a consistent set of tools and options so that the user can navigate easily and intelligently in and between the various courses and modules in the ATLAS-plus world. The program is a collaboration between anatomy and cell biology faculty, medical students, graphic artists, systems analysts, and instructional designers.

Anatomy↗
PubMed1988

A follow-up of the graduates of the Cardiff Bachelor of Nursing degree course.

This paper considers the career patterns of the graduates of the University of Wales College of Medicine Bachelor of Nursing course. The 4-year course commenced in 1972 and has now seen 11 years of graduates. A longitudinal questionnaire survey has been administered annually to graduates of the course to ascertain information relating to the posts held and qualifications gained by graduates, which is the focus of this paper. The questionnaire also elicits graduates' perceptions of the value of aspects of the degree course to posts held, and the reaction of colleagues to the fact that they are nurse graduates. The results of the follow-up are presented to show the posts held by graduates at four time intervals; 6 months, 2 years and 5 years following graduation, and all graduates at the 1986 follow-up. The numbers of those who have obtained various post-registration qualifications are also presented. The findings support those of other follow-up studies of nurse graduates and diplomats. The graduates largely entered nursing or related posts, with the majority remaining in clinical-based posts as opposed to teaching and administration. The findings lead to further questions regarding the role and function of the nursing graduate. Further study is currently in progress to compare various elements of the practice style of Bachelor of Nursing graduates with non-graduate nurses.

Career Choice↗
PubMed1988

A study to compare the practice style of graduate and non-graduate nurses and midwives: the pilot study.

This paper comprises essentially a discussion of the pilot of a study currently being undertaken to compare the practice styles of graduate and non-graduate nurses. The background to the main study is described and discussed, as is the methodology. The problems encountered in the planning and executing of the data collection are particularly highlighted. The paper does not present data from the pilot study, but evaluates the methodology used and considers the implications for the main study.

Education, Nursing, Baccalaureate↗
PubMed1988

A follow-up of the graduates of the 3-year post-registration Bachelor of Nursing degree course in the University of Wales.

This paper considers several aspects of the graduates of the 3-year Bachelor of Nursing course at the University of Wales College of Medicine. These include career progression, perceptions of the usefulness of aspects of the course, and the reactions of others to the fact that they have a degree. Information was elicited by a postal questionnaire which is administered annually to all graduates. The findings of the study are clearly limited due to a small sample size. They do, however, indicate that the graduates of the course so far have stayed in nursing posts, largely in teaching and administration, and the loss to the profession has been extremely small. Overall, the graduates found many aspects of the course very useful to posts held, and experienced various reactions of other professionals to the fact that they had a degree. Due to the relatively short time that this course has been in existence and the fact that the first groups were very small in number, the findings of the study merely suggest trends in the career patterns and perceptions of graduates which need to be continually monitored before statements can be made with confidence. They do, however, have implications when considering the function and contribution of these nurse graduates to the nursing profession.

Career Choice↗
PubMed1986

Dermatitis herpetiformis. Cutaneous deposition of polyclonal IgA1.

Dermatitis herpetiformis (DH) is a pruritic papulovesicular skin disorder of unknown cause, characterized by granular IgA deposits in the dermis along the dermoepidermal junction. It is associated with gluten-sensitive enteropathy and increased IgA production by gut lymphoid tissue. We report four cases of immunologically documented DH studied by immunofluorescence technique. Monoclonal antibodies against the IgA subclasses IgA1 and IgA2 were used. IgA1 without IgA2 was found in the cutaneous deposits in each case. The IgA1 had both kappa and lambda light chains in approximately equal quantities. Because normal gut-associated lymphoid tissue produces 70% IgA1 and 30% IgA2, while circulating IgA is primarily IgA1, it could be concluded that the IgA in the skin of DH patients is not produced in the gut. However, the subclass restriction of the IgA produced by pathologic gut-associated lymphoid tissue is unknown. Alternatively, both IgA1 and IgA2 may be produced by the gut, but only IgA1 is involved in the production of cutaneous lesions.

Animals↗
PubMed1985

Nurses' education.

Explore the source record for details and available documents.

Education, Nursing↗