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Biomedical subjects

C Leonardi

Publications and source records attributed to C Leonardi.

At least 55 records · Page 3Linked to original sources
PubMed1992

Multicenter clinical evaluation of a new SSIR pacemaker.

A multicenter clinical evaluation of Sorin Swing 100, a new SSIR pacemaker with a gravimetric sensor, was performed by seven different centers enrolling a total of 89 patients, 56 men and 33 women, mean age 73.1 years, for pacemaker implantation (73 patients) or pacemaker replacement (16 patients). Pacing mode was VVIR in 73 patients and AAIR in 16. The behavior of pacing rate was evaluated 3 months after the implant by performing a 24-hour Holter monitor, an exercise stress test, and tests for the assessment of mechanical external interference (MEI). A physiological behavior of the paced rate was always observed during Holter monitoring. In 52 completely paced patients mean diurnal, nocturnal, and maximal heart rate were, respectively, 74.9 +/- 5.7 ppm, 58.1 +/- 5.8 ppm, and 113.4 +/- 12.7 ppm; a paced rate exceeding 100 ppm was reached on the average 5.6 times/Holter monitor. In all but two patients the sleep rate (55 ppm) was reached during the night or long resting time. During exercise stress test a direct correlation between the increase in pacing rate and the increase in workload was observed; the mean maximal heart rate reached in 49 completely paced patients was, respectively, 102.8 +/- 9 ppm in 17 patients who accomplished stage 1, 116.2 +/- 13.6 ppm in 28 patients who accomplished stage 2, and 133 +/- 6.7 ppm in 10 patients who accomplished stage 3 of the Bruce protocol. MEI testing never increased the pacing rate over the noise rate (10 ppm over the basic rate). In only seven patients the results obtained suggested to change the nominal set up of the pacemaker.(ABSTRACT TRUNCATED AT 250 WORDS)

Acceleration↗
PubMed1992

Swollen keratinocytes: a histologic marker of unusual human papillomavirus-type infection and immunosuppression.

Cutaneous papillomavirus infection is common in patients who are immunosuppressed. We describe swollen keratinocytes in the granular layer in lesions from four patients who had human immunodeficiency virus infection. These cells were similar to those described in skin lesions of epidermodysplasia verruciformis. Amplification of DNA from the lesions revealed an amplimer for human papillomavirus using a consensus primer for a highly conserved region of the L1 open reading frame; however, specific binding was not noted when radiolabelled probes for human papillomavirus types 6, 11, 16, 18, and 33 were used. We conclude that the presence of these distinctive swollen cells strongly suggests immunosuppression and quite possibly infection by a less common papillomavirus type.

Biomarkers↗
PubMed1991

Epidermodysplasia verruciformis-associated papillomavirus infection complicating human immunodeficiency virus disease.

Three males infected with the human immunodeficiency virus (HIV) were noted to have extensive flat warts of the face and/or body. In two there were also pityriasis versicolor-like lesions. Biopsies showed foamy, basophilic, distended cytoplasm in granular layer keratinocytes, characteristic of the human papillomavirus types seen in epidermodysplasia verruciformis. DNA hybridization techniques demonstrated the presence of HPV-type 8 in one patient and HPV 5 and 8 in another. Patients with immune suppression due to HIV infection may demonstrate the clinical features of epidermodysplasia verruciformis with the same potentially oncogenic HPV types.

AIDS-Related Complex↗
PubMed1991

Epidermolytic acanthoma does not contain human papillomavirus DNA.

The histologic changes of epidermolytic acanthoma can mimic those of human papillomavirus infection. Sections from paraffin-embedded tissues identified histologically as epidermolytic acanthoma were examined for the presence of human papillomavirus (HPV) types 6, 11, 16, 18, and 33 using the polymerase chain reaction followed by dot-blot analysis with specific radiolabelled detecting probes. Although present in appropriate controls, human papillomavirus DNA was not detected in epidermolytic acanthoma. We conclude that epidermolytic acanthoma is not a skin lesion that is associated with human papillomavirus infection.

Blotting, Southern↗
PubMed1991

Irritated seborrheic keratoses and benign verrucous acanthomas do not contain papillomavirus DNA.

Sections from paraffin-embedded tissues of lesions thought to represent verrucous lesions (benign verrucous acanthomas: BVA) as well as materials from controls including seborrheic keratoses (SK), irritated SK, and verruca vulgaris from areas other than the genitalia were analyzed for the presence of multiple human papillomaviruses (HPV) including types 6, 11, 16, 18, and 33 using DNA amplification methodology. HPV DNA sequences were not found in amplified samples of BVA, SK, and irritated SK samples, but were identified in those obtained from verruca vulgaris. Our results suggest that BVA, SK, and irritated SK are generally not associated with HPV DNA even though certain of these lesions may histologically mimic the architecture of verruca.

Blotting, Southern↗
PubMed1991

Enhanced percentage of Leu M3+DR+ and Leu M3+CD25+ cells in newly diagnosed IDDM patients.

The percentage of Leu M3+DR+ and of Leu M3+CD25+ cells was determined by means of immunofluorescence analysis in a group of patients with insulin dependent diabetes mellitus (IDDM). Our results show that an increased percentage of these cells may occur in the early stage of the disease. These data provide evidence for a "phenotypical" activation of Leu M3+ cells at the onset of the disease and warrant future studies to evaluate the potential role of these cells in the pathogenesis of IDDM.

Adolescent↗
PubMed1990

Baseline and post-atrial pacing release of atrial natriuretic factor in mitral stenosis.

To investigate the release of atrial natriuretic factor (ANF) in mitral stenosis and the influence of the increase on the frequency of atrial contraction or atrial distention on ANF secretion, we studied 10 patients with symptoms of congestive heart failure (New York Heart Association classes II and III) in sinus rhythm, who were undergoing cardiac catheterization as part of an evaluation workup for mitral stenosis. Echocardiographic tracings, repeat determinations of mean pulmonary artery wedge pressure (MPAWP) and mean right atrial pressure, and blood sampling from the pulmonary artery for measurements of ANF were performed at baseline, during atrial pacing (pacing rate of 125 beats/min for 5 minutes), and 5 minutes after the pacing protocol was completed. Baseline ANF levels were closely related to right atrial pressure (r = 0.89; p less than 0.001) and increased markedly after atrial pacing from 205.6 +/- 39.8 (SEM) to 343.9 +/- 57.9 (SEM) pg/ml. A similar pacing-induced increase was shown for MPAWP and left atrial size. Our data indicate that pacing-induced increases in atrial distention and intracavitary pressure further stimulate release of ANF. However, an independent effect of frequency of atrial pacing on plasma ANF in humans could not be identified.

Adult↗
PubMed1990

[Osteosynthesis of radial head fractures in adults].

The authors report their experience and the results of internal fixation of radial head fractures in adults. They studied type 2 and type 3 fractures of Mason classification. Results were appraised with criteria of Radin and Riseborough. They found a rate of 80 per cent of objectively good or fair results. Fractures with one fragment (type 2) and without associated lesions (like sprain or dislocation of elbow) had the best results which depended of the quality of internal fixation. The series reported in the literature using the same operative technique are comparable. The results are equal or better than head resection with or without arthroplasty. Internal fixation is considered as the best operative technique for displaced fractures in adults. Reduction must be perfect. Head resection must be considered per-operatively if a perfect reduction is technically impossible to achieve.

Adolescent↗
PubMed1990

Digestive disturbances in shift-workers: a clinical statistical investigation.

In order to give a clarifying contribution about the probable relationship between shift-work and digestive diseases the authors have conducted a clinical and anamnestical investigation on digestive disturbances and alimentary habits of shift-workers comparing them with non shift-workers. The former group of subjects is engaged in very different activities conducted in variable working environments, with shift rhythms which are always disordered, sometime are imposed by the working Companies and sometimes chosen on the basis of personal necessities. The most interesting result of this study is represented by the confirmation of statistical significance among the larger incidence percentage of digestive disturbances between shift and non shift-workers. Moreover, statistical differences have been remarked among the different groups of shift-workers examinated. For these reasons the authors believe that shift-work is per se responsible of digestive diseases in the workers engaged to it and they also think that it is very important to distinguish the kind of shift work which may produce environmental, psychological and motivational differences.

Adaptation, Physiological↗
PubMed1989

Evidence for a further stimulation of atrial natriuretic factor release by atrial pacing in patients with mitral stenosis.

To investigate the release of atrial natriuretic factor (ANF) in mitral stenosis and the effect of an increased atrial contraction frequency on atrial distension and ANF secretion, we studied 14 patients [New York Heart Association (NYHA) grades II-III] in sinus rhythm, undergoing cardiac catheterization for mitral stenosis. Echocardiographic tracings, repeat determinations of mean pulmonary artery wedge pressure and blood samples from the pulmonary artery for ANF measurements were taken at baseline, during atrial pacing (125 beats/min for 5 min) and 5 min after pacing. After pacing, ANF levels rose markedly with a parallel increase in mean pulmonary artery wedge pressure and left atrial size. These data indicate that atrial pacing is capable of further stimulating ANF release, even in patients with elevated baseline ANF and left atrial pressure and an increased left atrial dimension.

Adult↗
PubMed1989

Mosquito salivary gland antigens identified by circulating human antibodies.

Salivary glands were removed from female mosquitoes of laboratory-reared strains of species common to southern Florida. Protein antigens were isolated from homogenates of these glands by denaturing electrophoresis and transferred to nitrocellulose for immunoblotting with serum samples obtained from human volunteers. A spectrum of antigens with a wide range of molecular weights (14 to 126 kilodaltons) were identified by antibodies in human serum for each species. Both species-unique and species-shared antigens were present. The results of these studies indicate that the humoral response to mosquito bite is complex, with a multitude of antigens provoking antibody responses. Since each individual has his or her own unique exposure history to mosquitoes, it seems unlikely that desensitization to a specific immunogen will confer protection in clinical situations in which multiple exposures to a variety of mosquito species occur.

Animals↗
PubMed1988

Confirmation of early Kaposi's sarcoma by polyclonal antibody to type IV collagen.

By the use of polyclonal antibody to type IV collagen that binds to epitope(s) retained in routinely processed tissues, we have confirmed the presence of early Kaposi's sarcoma in skin lesions that were not diagnostic on histologic examination. This reagent is of considerable use, particularly in cases of rapidly developing Kaposi's sarcoma with acquired immunodeficiency syndrome in which histologic features may not be fully developed.

Antibodies↗
PubMed1987

Peptic disease and 24-hr patterns of serum gastrin and pepsinogen.

The present study explored the 24-hr variations in serum gastrin and pepsinogen in clinically healthy subjects and in patients with gastric ulcer, duodenal ulcer, and erosive gastroduodenopathy. Time-qualified data were analyzed by means of cosinor procedures. Significant changes in rhythmometric properties were documented in patients with peptic disease when compared to clinically healthy subjects. In essence, it was discovered that gastric ulcer patients exhibit a higher mesor and amplitude for both gastrin and pepsinogen, whereas duodenal ulcer patients and those with erosive gastroduodenopathy show only a significant increase in the pepsinogen mesor. These characteristics are so specific in the groups investigated that one can hypothesize that the disorders in the circadian rhythmicity of gastrin and pepsinogen have a role in determining the clinical manifestations of peptic disease.

Circadian Rhythm↗