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Biomedical subjects

C Harris

Publications and source records attributed to C Harris.

At least 253 records · Page 14Linked to original sources

Antenatal diagnosis in practice.

Over a period of 5 years, 434 women at risk of having abnormal babies have had antenatal daignostic tests carried out during the first half of their pregnancy by the laboratories of the Department of Human Genetics, University of Cape Town. From these investigations, it was predicted that 13 fetuses had chromosomal abnormalities, 6 had severe central nervous system defects and 4 had autosomal recessive metabolic disorders. In addition, 4 cases with X-linked recessive traits were monitored and 3 male fetuses were recognized. Affected pregnancies were terminated except for 1 with a fetal sex-linked disorder where the parents revoked their original decision. The diagnosis was confirmed by fetal autopsies in all cases except 4 (2 spontaneous abortions and 2 out-of-town terminations). There was only 1 case where culture failed and the pregnancy went to term with the birth of a baby with Down syndrome. Antenatal diagnosis is now an important part of normal clinical practice. The fact that the fetal abnormalities were recognized in 6% of pregnancies is justification for the use of this procedure.

Amniocentesis↗

Diagnosis of occult familial medullary carcinoma of the thyroid using pentagastrin.

Medullary carcinoma of the thyroid is unique in that it can often be diagnosed solely on the basis of abnormally high basal serum thyrocalcitonin levels. Two brothers described in this report were found to have normal basal serum thyrocalcitonin levels when examined after another family member died from metastatic medullary carcinoma of the thyroid. Pentagastrin, a synthetic analog of gastrin, was subsequently administered to these two high-risk relatives and found to stimulate the release of thyrocalcitonin to abnormally high levels. They were found to have occult medullary carcinoma of the thyroid. Eight other first-degree relatives of the deceased patient with normal basal serum thyrocalcitonin levels were screened with pentagastrin and found to have normal stimulated levels of thyrocalcitonin. This report reinforces the need to recognize that medullary carcinoma of the thyroid can be familial and that physicians are obligated to screen asymptomatic kin who are at high risk. We recommend that all susceptible relatives, even if they have normal basal serum thyrocalcitonin levels, undergo the provocative pentagastrin test with repeat annual testing if necessary. In addition, the provocative pentagastrin test can be used to follow up patients who have undergone thyroidectomy.

Adolescent↗

Acute microwave irradiation and cataract formation in rabbits and monkeys.

Rabbits and monkeys were irradiated in the near field of a cavity-backed 2450 MHz resonant slot radiator, to determine the cataractogenic threshold. Rabbits developed cataracts at incident "apparent" power densities of 180 mW/cm2 (E2/120 pi, where E=rms/electric field strength). Monkeys sustained facial burns, but no lens damage, even at incident "apparent" power densities of 500 mW/cm2. These results were substantiated by computer thermal models.

Animals↗

Experimental pancreatic carcinogenesis. II. Lifetime carcinogenesis studies in the outbred Syrian golden hamster with N-nitroso-bis(2-hydroxypropyl)amine.

A high incidence of pancreatic duct neoplasms was induced in outbred male Syrian golden hamsters following weekly sc injection of N-nitroso-bis(2-hydroxypropyl)amine for life. The first such tumors appeared as early as 16 experimental weeks; the maximum incidence reached 100% by the termination of the study. Tumors in the respiratory tracts and angiosarcomas of the livers of the hamsters were also observed in high frequency. Latency of the induced neoplasms was significantly decreased by the substitution of distilled water for olive oil as the vehicle for the carcinogen.

Animals↗

Labetalol in the difficult hypertensive patient in general practice.

An open study was carried out in general practice to assess the effectiveness and tolerance of labetalol in the treatment of 53 difficult hypertensive patients, most of whom had failed to respond to previous hypotensive therapy. The results showed that in the 49 patients receiving treatment for more than 3 months good blood pressure control was readily achieved, and with labetalol alone in all but 6 of them. The majority were controlled on doses ranging from 300 to 600 mg per day. Treatment was discontinued in 3 patients because of severe side-effects. Mild, tolerable side-effects, similar to those found with beta-adrenergic blocking agents, were reported by 35 patients.

Adult↗

Patterns of second malignant neoplasms in children.

A search of the records of 10 pediatric oncology centers revealed 102 children with more than one malignant neoplasm. In this group of 102 patients, all pediatric cancers were seen as initial lesions, but Wilms' tumor and retinoblastoma were over-represented and leukemia and brain tumors underrepresented. Survival variation as well as tumor susceptibility may be responsible for this disproportion. Osteosarcomas and chondrosarcomas were the most frequent second malignant neoplasms (SMN). Embryonal tumors were rare as SMN and adult-type tumors (carcinomas) appeared at earlier than expected ages, whether arising after irradiation or not related to that form of therapy. Radiation was associated with 69 SMN, genetic disease accounted for 27 SMN and both conditions were noted in 15 SMN. In the group of 21 patients for whom neither radiation nor a known genetic disorder could be implicated, there were three with colon carcinoma and glioma and five with leukemia or lymphoma and glioma. These combinations may reflect new tissue-specific hereditary cancer syndromes.

Adolescent↗

Benzo(a)pyrene diol epoxides as intermediates in nucleic acid binding in vitro and in vivo.

Evidence has been obtained that a specific isomer of a diol epoxide derivative of benzo(a)pyrene, (+/-)-7 beta,8alpha-dihydroxy-9alpha, 10alpha-epoxy-7,8,9,10-tetrahydrobenzo(a)pyrene, is an intermediate in the binding of benzo(a)pyrene to RNA in cultured bovine bronchial mucosa. An adduct is formed between position 10 of this derivative and the 2-amino group of guanine.

Animals↗

Decreased risk of radiation-associated second malignant neoplasms in actinomycin-D-treated patients.

One hundred two long-term survivors of childhood cancers with second malignant neoplasms (SMNs) were collected from 10 institutions. Forty-seven cases fulfilling study criteria were studied to determine the risk of developing a SMN in irradiated fields after exposure to various chemotherapeutic agents. The case control method was used. The risk of developing such tumors was decreased by a factor of 7 in patients receiving actinomycin-D (AMD). The "protective" effect of AMD was more pronounced in patients receiving repeated courses of the agent. No change in relative risk was found for children given antifolates, the vinca alkaloids, or alkylating agents. AMD "protection" is an unexpected finding because the agent is an oncogen in animals and an enhancer of radiation, the latter being a known carcinogen. Possible mechanisms, which remain speculative, are discussed. These results indicate the need for careful long-term observation of cancer survivors to gain understanding of the late effects of multimodal treatments.

Child↗

Chemical carcinogenesis and experimental models using human tissues.

The majority of human cancer is caused by environmental agents including chemical carcinogens. These chemical carcinogens are both naturally-occurring compounds and products of an industrialized society. Most of the environmental chemical carcinogens require enzymatic activation into electrophilic metabolites which readily react with cellular macromolecules leading, in some cases, to malignant transformation of target epithelial cells. Model systems for studying carcinogenesis in human target tissues are being developed to link investigations using experimental animals with human cancer. Metabolism of chemical carcinogens is being studied in human tissues maintained in these model systems. For example, a marked variation among individuals in their capacity to metabolize carcinogenic polynuclear aromatic hydrocarbons has been observed. This inter-individual variation may, in part, be due to genetic factors. Model systems using human tissues should be useful in several areas of cancer research including: 1) identifying environmental carcinogens, their metabolic pathways and host factors determining susceptibility; and 2) testing new methods of prophylatic intervention in populations at high risk of developing cancer.

Binding Sites↗