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C Griscelli

Publications and source records attributed to C Griscelli.

At least 343 records · Page 19Linked to original sources
PubMed1969

The distribution of large dividing lymph node cells in syngeneic recipient rats after intravenous injection.

The distribution of large dividing lymph node or thoracic duct lymph cells, labeled in vitro with (3)H-thymidine, was studied in syngeneic recipient rats after intravenous injection. In most experiments the donor rats had been immunized with Bacillus pertussis 4 days earlier, but in some instances cells from nonimmunized donors were used. In smears, the labeled donor cells had the appearance of large lymphocytes or large pyroninophilic cells. By electronmicroscopy, the majority of labeled donor cells were seen to have only scanty endoplasmic reticulum. It was found that the labeled cells rapidly "homed" to lymphoid tissue and recirculated in the recipient, in a fashion resembling that of small lymphocytes. However, the distribution of labeled cells was found to depend upon the source of the donor cells. Cells from mesenteric lymph nodes or thoracic duct lymph showed a marked preferential accumulation in lymphoid tissue within or adjacent to the intestine, whereas cells from peripheral nodes accumulated preferentially in peripheral lymph nodes. Cells from any of these sources showed an equal tendency to accumulate in the white pulp of the spleen. Suspensions of small lymphocytes, labeled in vitro with (3)H-uridine, did not display a similar tendency to localize preferentially in lymphoid tissue in certain regions. It was also found that large dividing lymph node cells from donors immunized with an antigen (2,4-dinitrophenyl-bovine gamma globulin (DNP-BGG) or B. pertussis) showed a greater tendency to accumulate in a recipient lymph node containing that antigen than in the contralateral node. It was not determined whether the selective accumulation of large dividing lymphoid cells from different sources in lymphoid tissue of different regions in recipients was due to an antigen recognition mechansim or was the result of two different populations of cells with different "homing" mechanisms.

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Immune response to Toxoplasma gondii in man. I. Common idiotypic determinants of toxoplasma-specific human antibodies.

A rabbit antiserum against human anti-toxoplasma antibodies was prepared. The rabbit antiserum was made antiidiotypic by extensive absorption. It bound 70% of radio-labelled autologous antibodies. The same antiidiotypic antiserum bound to varying degrees, three out of four other anti-toxoplasma antibodies isolated from different individuals. Inhibition experiments, using 24 human sera containing varying amounts of antibodies, showed that about 60% of them cross-reacted with the antiidiotypic antiserum, indicating that some common determinants were present on human anti-toxoplasma antibodies. F(ab')2 fragments of antiidiotypic immunoglobulin revealed about 8% idiotype-positive (id+) cells among toxoplasma-induced blasts obtained from toxoplasma-sensitized normal donors.

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