Search PubMed⌕ Search

Biomedical subjects

C E Yale

Publications and source records attributed to C E Yale.

49 records · Page 3Linked to original sources
PubMed1970

Intestinal Strangulation in Escherichia coli-Monocontaminated Gnotobiotic Rats.

The natural course of ischemic and hemorrhagic intestinal strangulation in previously germ-free rats individually monocontaminated with one of five separate strains of Escherichia coli was investigated. In addition, the effect of intraintestinal homologous heparinized blood upon the course of ischemic intestinal strangulation in E. coli-monocontaminated rats was studied. It was found that (i) E. coli could be an important lethal factor in ischemic and hemorrhagic intestinal strangulation, (ii) hemorrhagic was more deadly than ischemic strangulation, (iii) intra-intestinal blood did not augment the action of the E. coli in these experiments, and (iv) some strains of E. coli were more toxic than others in the presence of intestinal strangulation.

Journal Article↗
PubMed1969

Vacuum-cleaning system for isolation chambers.

To encourage the utilization of the isolation chamber as a research tool, the cost of its use should be lowered. Methods and devices must be developed which make more efficient use of the space within the isolator and allow the operator to work more effectively in this confined area. A simple vacuum-cleaning system is described; it consists of a nozzle and flexible hose which connect through the isolator wall to an externally placed waste tank, attached by way of its outlet filter to a source of vacuum. The cylindrical waste tank [48 inches (1.219 m) high and 36 inches (0.914 m) in diameter] was sterilized in a large autoclave. During a 9-month test period, the system was used to remove soiled corncob bedding from a large isolator containing 90 adult monocontaminated rats. During this period, the microbial flora of the isolator was unchanged, and the time required to clean the cages was reduced by 50%. This vacuum-cleaning system is a safe, convenient, and economical means of increasing the efficiency of an isolation chamber.

Animals↗
PubMed1968

Air filters for germ-free isolators.

A germ-free isolator must have a perfect bacterial filter. This paper describes a new, relatively inexpensive, stainless-steel filter frame, which is easily and quickly assembled and protects the enclosed filter material at all times. Resistance to the flow of air was less than 4 inches of water at an airflow of 30 ft(3)/min through the filter frame with 204 inches(2) of surface area and four, one-half inch thick pieces of fiberglass filter material. This filter performed satisfactorily in our gnotobiotic laboratory and was found to be consistently 100% efficient in removing an aerosol containing Serratia marcescens from an air stream under a variety of operating conditions.

Air Microbiology↗
PubMed1968

Heat sterilization of water in a large open vessel.

A safe, convenient, and economical method of preparing and dispensing a large volume of sterile water in a movable container is described. A caster-mounted, rectangular, 100-gal, stainless-steel water tank was fabricated. An audible, solid-state water-level alarm was developed for use with a detachable sensing probe that could be autoclaved. A filter system was constructed to allow the tank to be autoclaved as an open vessel. Thermocouples were mounted within the tank of water to study the time-temperature relationships of the water during the sterilization cycle. In a downward displacement autoclave with a hot jacket, 75 min were required for the water temperature to rise from 140 to 240 F (60 to 116 C). A total of 3 hr for heating and holding includes an adequate safety factor to insure the sterility of the water immediately after autoclaving. The long-term sterility of the water and the safety of the system were verified by using the water to maintain a germ-free animal colony.

Hot Temperature↗