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Biomedical subjects

C D Dijkstra

Publications and source records attributed to C D Dijkstra.

153 records · Page 9Linked to original sources
PubMed1983

Ontogenetic development of T- and B-lymphocytes and non-lymphoid cells in the white pulp of the rat spleen.

The location and temporal appearance of the different classes of lymphocytes were investigated in the developing white pulp of the rat spleen. Additionally, indications were sought for the involvement of non-lymphoid cells in the localization of lymphocytes. The lymphocytes were demonstrated by their surface determinants (W3/13, IgM, IgG, IgA, Ia) in a two-step immunoperoxidase method; the non-lymphoid cells were characterized by immuno-enzyme-histochemical techniques. The results showed that 1) already at birth strongly Ia-positive cells are present in the T-cell area; 2) the marginal zone develops as a distinct compartment, independent of the PALS and follicles; 3) follicles are recognizable at day 14, the capacity to trap immune complexes on follicular dendritic cells occurring one week later. A possible relation between the development of the follicles and the differentiation of the follicular dendritic cell is discussed.

Age Factors↗
PubMed1983

Regeneration of splenic tissue after autologous subcutaneous implantation: homing of T- and B- and Ia-positive cells in the white pulp of the rat spleen.

The localization of T- and B lymphocytes and interdigitating cells (IDC) was investigated during the regeneration process of splenic implants. For this purpose a two-step immunoperoxidase technique was used to visualize T-cell antigen, immunoglobulins and Ia-antigen on cryostat sections. The specific localization of the repopulating lymphocytes occurred simultaneously with the development of non-lymphoid elements characteristic for the different compartments of the white pulp, i.e., the periarteriolar lymphocyte sheaths (PALS) and follicles. The marginal zone (MZ) developed after the PALS and primary follicles, but before germinal center reactions were found. During ontogeny, however, the development of a broad MZ precedes the formation of follicles. This difference in sequence of events is discussed.

Animals↗
PubMed1983

Localization of horseradish peroxidase (HRP)-anti-HRP complexes in cryostat sections: influence of endotoxin on trapping of immune complexes in the spleen of the rat.

Cryostat sections of the rat spleen were incubated with a medium containing horseradish peroxidase (HRP)-anti-HRP complexes. After this incubation HRP-anti-HRP complexes were demonstrated in the follicle centres in the splenic white pulp. The localization pattern of the HRP-anti-HRP complexes after incubation ("in-vitro trapping") was compared with the localization pattern of 125I BSA-anti-BSA complexes after intravenous injection ("in-vivo trapping"). Furthermore, the influence of endotoxin on the "in-vitro trapping" was studied. The "in-vitro trapping" was representative of the second, retaining phase of the "in-vivo trapping". Evidence is presented that endotoxin inhibits trapping of immune complexes by a direct effect on the follicular dendritic cell.

Animals↗
PubMed1983

The effectiveness of 3H-uridine and 3H-leucine for labeling of T and B cells of rats and mice.

Using a technique for combined autoradiography and immunoperoxidase staining, we studied the incorporation of 3H-Uridine and 3H-Leucine into T and B lymphocytes of rats and mice. While in mouse both T and B cells were clearly labeled by incorporation of 3H-Uridine, a portion of rat T cells remained unlabeled. Furthermore, the majority of rat B cells was not labeled. In contrast, mouse T and B cells as well as rat T and B cells could be labeled with 3H-Leucine to a comparable degree.

Animals↗
PubMed1982

Regeneration of splenic tissue after autologous subcutaneous implantation: development of non-lymphoid cells in the white pulp of the rat spleen.

Regeneration of splenic tissue after autologous subcutaneous implantation provides a useful model for studying the development of splenic tissue. The development of the various non-lymphoid cells of the white pulp in the rat is described. It appears that regeneration of the implants is initiated by ingrowing vessels and a newly formed reticulum, which forms the microenvironment for the homing lymphocytes. Marginal metallophils are found at their characteristic location at the inner border of the marginal sinus five weeks after implantation. Trapping of antigen-antibody complexes reappears when the first primary follicles can be recognized.

Acid Phosphatase↗
PubMed1982

Ontogenetic aspects of immune-complex trapping in the spleen and popliteal lymph nodes of the rat.

An ontogenetic approach was used to obtain information about the relation between structure and function of lymphoid tissues. In particular the development of the capacity to trap immune complexes was studied in relation to the development of the lymphoid compartments. For this purpose isologous horseradish (HRP)-ant-HRP complexes were injected into neonatal rats, and their fate was studied in the spleen and popliteal lymph nodes. Immune-complex trapping occurred as soon as primary follicles could be recognized; without follicles no trapping was observed. Several explanations for this simultaneous development of trapping capacity and follicular structure are discussed.

Animals↗
PubMed1982

Characterization of nonlymphoid cells in rat spleen, with special reference to strongly Ia-positive branched cells in T-cell areas.

By use of a monoclonal antibody against Ia antigen in an immunoperoxidase method, strongly Ia-positive branched cells are found in the T-cell areas of the splenic white pulp of the rat. In order to further characterize these cells, enzyme histochemical characteristics, phagocytic capacity, and irradiation sensitivity have been studied. Evidence is presented that these strongly Ia-positive branched cells represent interdigitating cells. The influence of whole-body irradiation on interdigitating cells is discussed. Comparison with data from the literature on the in vitro dendritic cell isolated from spleen cell suspensions reveals many similarities between the described interdigitating cell in vivo and the dendritic cell in vitro.

Acid Phosphatase↗