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Biomedical subjects

C Chastel

Publications and source records attributed to C Chastel.

At least 199 records · Page 11Linked to original sources
PubMedDate not supplied

Polypeptide cartography of Spiroplasma taiwanense.

Spiroplasma taiwanense is the first member of the Class Mollicutes to be subjected to polypeptide cartography using computerized image analysis. The small genome size characteristic of this group was shown to code for low numbers of polypeptides when compared to other bacterial species. Silver-stained two-dimensional electrophoresis gels, following separation by either isoelectric focusing and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (ISO-DALT) or nonequilibrium pH gradient electrophoresis (NEPHGE), were used to create databases from 10 and 6 gels, respectively, for each technique and produced, respectively, 263 and 287 replicated spots. Polypeptides were mapped with respect to molecular mass and glyceraldehyde-3-phosphate dehydrogenase carbamylation standards. Of interest was the unexpectedly high percentage (50.2%) of the total normalised optical intensity associated with all 263 spots detected by ISO-DALT electrophoresis, having been contributed by just 29 dominant protein spots. These 29 polypeptides are to be given priority in microsequencing and microanalysis aimed at their identification.

Bacterial Proteins↗
PubMedDate not supplied

[Edmond Nocard (1850-1903) and the centenary of the discovery of the first mycoplasma (1898)].

In 1898, E. Nocard from the Alfort National Veterinary School and his colleagues from the Pasteur Institute have succeeded in growing the bovine pleuro-pneumonia agent, using small bags of collodion inserted into the peritoneum of rabbits. Unfortunately, the cultured micro-organism was considered by us as a filterable virus. The confusion between true viruses and mycoplasmas has persisted for a long time and delayed the development of the modern concept of virus until the 1940's.

France↗
PubMedDate not supplied

[Selection of a herpes simplex mutant virus of weak pathogenicity, sensitive to acyclovir and not susceptible to reactivation in vivo].

From a KOS herpes simplex virus strain prepared in VERO cells we have been able to obtain after several exposures to increasing concentrations of trifluorothymidine, four resistant mutants. Among these strains, 31 T mutant was a TK-altered strain of low pathogenicity, sensible to acyclovir and did not induce recurrences. These properties might allow the use of such a strain as a living attenuated antiherpetic vaccine.

Acyclovir↗