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C Bueno

Publications and source records attributed to C Bueno.

53 records · Page 3Linked to original sources
PubMedDate not supplied

[The most significative parameters for the diagnosis of focal neuropathy of the cubital nerve in the elbow].

OBJECTIVE: Determine the diagnostic validity of the most usual neurophysiological procedures in the examination of compressive neuropathies and/or ulnar nerve entrapment at the elbow. PATIENTS AND METHODS: A prospective observational study was carried out over a period of one year which included 19 patients with clinical features compatible with entrapment/compression of the ulnar nerve at the elbow, their neurophysiological diagnosis and surgical confirmation. We also studied 49 patients who were attended in our clinic for other reasons not related to disorders of the ulnar nerve. The variables studied were: maximum rate of motor conduction above the elbow; the amplitudes between spikes and the area between the first negative deflection and the last positive spike of the responses at the wrist and above the elbow. RESULTS: In the group of persons without disorders of the ulnar nerve the average rate of motor conduction was 58.07 m/s, with a confidence interval of 53-65. In the group of patients with specific clinical features the average rate of motor conduction was 47.26 m/s, with a confidence interval of 43.74-50.77. No relation was found between age and pathology, although rate of motor conduction was related to the presence of pathology. CONCLUSIONS: It was seen on logistical regression that the main variables for prediction of the presence or absence of pathology in a person were firstly motor conduction and secondly the amplitude index.

Adolescent↗
PubMedDate not supplied

Epidermal nuclear immunoglobulin deposition in connective tissue diseases.

Epidermal nuclear deposition of immunoglobulins (in vivo ANA) was observed in 45 out of 252 skin biopsies (17.8%). It occurred in 19% of cases with systemic lupus erythematosus, in 32% of the mixed connective tissue disease, in 22% of the scleroderma, in 20% of the cutaneous vasculitis, in 18% of the polymyositis, in 33% of the Sjogren's syndrome, but it was absent in cases with rheumatoid arthritis. The in vivo ANA showed a significant association with serum antibodies to an extractable nuclear antigen (ENA), with speckled pattern of immunofluorescent antinuclear antibody (FANA) and with antibody to a fraction of ENA sensitive to ribonuclease termed ribonucleoprotein (RNP). Indirect evidence was obtained suggesting that the epidermal nuclear deposition of immunoglobulins is a true in vivo phenomenon: some patients with serum antibodies to ENA do not display in vivo ANA and contrariwise, no difference was detected between diseased and normal skin for the occurrence of in vivo ANA and also no association was observed between this phenomenon with immune deposits at dermoepidermal junction or in subepidermal vessels.

Antibodies, Antinuclear↗
PubMedDate not supplied

Anticardiolipin antibodies and disease activity in systemic lupus erythematosus.

We studied a group of 80 unselected patients with systemic lupus erythematosus (SLE). Twenty six (32.5%) of them were considered in clinical activity using criteria based on clinical features and laboratory abnormalities. Using an isotype specific enzyme linked immunosorbent assay (ELISA) for anticardiolipin antibodies we found IgG anticardiolipin in 28/80 (35%) and IgM anticardiolipin in 20/80 (25%) patients. Antibodies to native DNA by immunofluorescence were found in 11/80 (14%) patients, whereas antibodies to denatured DNA by ELISA were found in 51/80 (64%) patients. There was an association between disease activity and the presence of anticardiolipin and anti-DNA antibodies particularly of the IgG isotype. We also found an association between anticardiolipin and anti-DNA suggesting a cross reaction between these antibodies, but we are not able to exclude the possibility of a simple coincidental phenomenon due to polyclonal synthesis of immunoglobulin observed during periods of disease activity.

Adolescent↗
PubMedDate not supplied

[Clinical and biochemical characterization of isoniazid-induced auto-antibodies].

Isoniazid (INH) is one among many drugs capable of inducing autoantibodies and, in some cases, a lupus-like syndrome (LE). A longitudinal study was performed in 24 tuberculosis patients treated with INH to detect antibodies (A-AH) to total histones and fractions. Antinuclear antibodies were observed in two patients after treatment. Higher frequency of IgM-AH was also observed. IgM-AH binding to all fractions were observed in those serum samples exhibiting stronger ELISA reactivity. Conversely, binding to only H1 occurred when lower IgM-AH activity was tested. Correlations with clinical expressions of LE were not observed in the present study.

Adolescent↗
PubMedDate not supplied

Association of anti-DNA and anticardiolipin antibodies in systemic lupus erythematosus.

Recent studies have shown that anti-DNA and anticardiolipin antibodies in patients with SLE may cross-react. Using an ELISA for determination of these antibodies, a strong association between IgG anti-denatured DNA and IgG anticardiolipin antibodies was found. Eight sera samples with the highest levels of both antibodies were selected to determine the possibility of a cross-reaction. The levels of anticardiolipin were not affected by denatured DNA in concentrations adequate to inhibit the anti-DNA binding. These data did not confirm previous studies using monoclonal antibodies showing cross reactivity between the two groups of antibodies. Nevertheless, a population of antibodies that may cross-reacts, in some special cases, cannot be ruled out.

Antibodies, Antinuclear↗
PubMedDate not supplied

[Facial granuloma].

Explore the source record for details and available documents.

Adult↗
PubMedDate not supplied

[Comparison between immunofluorescence techniques and ELISA, using whole neutrophil extract and primary granules, for the detection of antineutrophil cytoplasma antibodies (ANCA)].

The detection of antineutrophil cytoplasmic antibodies is a very important tool for the diagnosis of systemic vasculitis. The specificity and sensitivity of these antibodies depends on the assay utilized for their detection. Therefore we have compared the immunofluorescence test (IF) with the ELISA using two different antigens: total neutrophil extract and isolated primary granules. Two patterns of fluorescence were detected by IF: the classic pattern was highly specific for Wegener's granulomatosis. In contrast the perinuclear staining correlated with renal vasculitis but was also observed in other diseases. However the IF test was unable to differentiate low-positive from atypical patterns. Such distinction could be achieved by ELISA. The use of ELISA with isolated primary granules is a good alternative for if since it has a good specificity, sensitivity and reproducibility, moreover it is a quantitative method.

Antibodies, Antineutrophil Cytoplasmic↗
PubMedDate not supplied

[Antineutrophil cytoplasmic antibodies in systemic vasculitis].

The term systemic vasculitis concerns a group of diseases characterized by inflammation of vessels. The diagnosis and follow-up of these conditions is a serious challenge since their classification is difficult and the therapy is usually empiric. Perhaps the greatest breakthrough in the management of these diseases is the recent discovery of serological markers for some vasculitic syndromes. Wishing to determine how far the tests for detection of these antibodies are specific, sera from 63 patients with systemic vasculitides were tested by immunofluorescence of ethanol fixed neutrophils. Two different staining patterns were observed. The cytoplasmic staining (classic pattern) was very specific for Wegener's granulomatosis. In contrast, the perinuclear staining was also present in many vasculitic syndromes such as microscopic polyarteritis and poliarteritis nodosa. In addition, the sera were tested by ELISA, using whole neutrophil cell extract. Although the assay was less specific than the immunofluorescence, the test was helpful in discriminating between true ANCA and atypical fluorescence patterns. Moreover, high levels of antimieloperoxidase antibodies detected by ELISA in a limited number of patients were exclusively associated with the diagnosis of microscopic polyarteritis or Wegener's granulomatosis with renal involvement. Our data confirm the remarkable association of antineutrophil cytoplasmic antibodies with vasculitic syndromes.(ABSTRACT TRUNCATED AT 250 WORDS)

Adolescent↗