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Biomedical subjects

C Bueno

Publications and source records attributed to C Bueno.

At least 37 records · Page 2Linked to original sources
PubMed1998

[The usefulness of abdominal echography in the diagnosis of extrapulmonary tuberculosis in patients with HIV infection].

BACKGROUND: The aim of the present study was to analyze the diagnostic profitability of echography as an indicator of extrapulmonary tuberculosis in patients with HIV infection. PATIENTS AND METHODS: HIV positive patients presenting fever of long duration were prospectively studied with an active search for specific echographic lesions. Descriptive statistics were performed by variance analysis. The diagnostic profitability of echography was evaluated by the calculation of sensitivity, specificity, positive predictive values (PPV) and negative predictive value (NPV). RESULTS: Criteria of prolonged fever was fulfilled by 116 patients. Thirty-five (30.2%) presented specific echographic alterations: 12 had multiple hyoechoic splenic lesions (34.3%), 11 abdominal adenopathies (31.4%), 9 splenic lesions and adenopathies (25.7%) and 3 showed hepato-splenic involvement and adenopathies (8.6%). The final diagnoses of these patients were: one case of toxoplasmosis, 2 MAI infection, 7 with no definitive diagnosis, and 25 (71.4%) tuberculosis. The mean CD4 lymphocyte count was 46.6 x 10(6)/L in patients with tuberculosis with no echographic findings, with a statistically significant difference of p < 0.05. The appearance of some echographic alterations had a global sensitivity of 37.3%, a specificity of 79.6% a PPV of 0.65 and a NPV of 0.51. The isolated findings of hypoechoic splenic lesions showed a sensitivity of 19.23%, a specificity of 95.12%, a PPV of 0.83 and a NPV of 0.47. CONCLUSIONS: The presence of multiple hypoechoic splenic lesions showed an elevated specificity, being greater than 95%, making this finding, although infrequent, that of greatest diagnostic profitability in the echographic study of tuberculosis. We therefore consider abdominal echography to be of great usefulness in the evaluation of patients with HIV infection and prolonged fever since the presence of these lesions, in the most severely immunosuppressed patients, may strongly suggest the diagnosis of extrapulmonary tuberculosis.

Abdomen↗
PubMed1995

[Predictive factors for hospital infections caused by Gram-positive and Gram-negative organisms].

BACKGROUND: Knowing the bacterian map and clinical profile of nosocomial infections (NI) in Spain may aid the better planning of empiric antimicrobian treatment. METHODS: A prospective incidence study carried out over 9 months was performed. Data collection out with the use of an EPINE project file. The chi square test and comparison of independent sample percentages were used for statistical analysis. RESULTS: During the study period 156 cases of NI (rate (5.5%) were detected: 65 patients with gram-negative bacilli infection (GNB), 34 by gram-positive cocci (GPC), 20 with mixed infection and 13 by Candida. The most frequent localization was urinary infection (63%) followed by surgical wound infection, pressure ulcers and respiratory infection. Of the 203 isolations, 57% corresponded to GNB, with E. coli being the most frequent microorganism. Staphylococcus aureus was the GPC most often found (95% methycilline sensitive). CONCLUSIONS: The profile of a patient with nosocomial infection in a hospital such as that in which the autors work would be as follows: if the patient were admitted in the department of internal medicine, was dementia or coma, denutrition, urinary catheter or neurologic disease and has NI (overall urinary infection) the infection would most likely be a caused by a gram-negative microorganism. If the patient has an i.v. line or is in a surgical ward, or has deep surgical wound infection the microorganism isolated would most likely be gram-positive.

Anti-Bacterial Agents↗
PubMed1993

Performance characteristics of radioluminescent fiber optics as electron scintillators.

Terbium-activated radioluminescent fiber-optic face plates have been studied for their suitability as electron scintillators. The energy conversion efficiency of such material is determined to be about 2.5% in the electron energy range of 100-400 keV tested. The resolution is approximately 40 microns at electron energy of 100 keV, as measured by the rise width of an edge. The main advantage of such a device is the potential simplification of CCD camera designs, since it functions both as a scintillator as well as a light guide so that it can be coupled direct to a CCD detector.

Animals↗
PubMed1990

Antiphospholipid antibodies in syphilis.

1. An enzyme-linked immunosorbent assay was used to determine the phospholipid specificity of antibodies present in sera from 35 syphilis patients. 2. Based on the cross-reaction obtained against a mixture of cardiolipin, phosphatidylcholine and cholesterol that is standard for flocculation tests according to the Venereal Disease Research Laboratory (CECON, São Paulo, Brazil), all 35 patients tested positive for antibodies of the IgG class whereas 13 (37%) also had IgM antibodies for the same mixture of lipids. IgG antibodies to cardiolipin were demonstrated in 2 patients (6%) and IgM antibodies in 5 (15%). Significant levels of IgG anti-phosphatidylcholine were detected in 3 patients (9%) and IgM antibodies in 4 (11%). IgG anti-phosphatidylethanolamine antibodies were found in 1 patient (3%) and IgM antibodies in 3 (9%). Antibody binding to cardiolipin plus cholesterol or cardiolipin plus phosphatidylcholine was as effective as when the standard mixture of all 3 lipids was used. 3. A comparison with serum from systemic lupus erythematosus patients and inhibition studies using liposomes of cardiolipin or the mixture of 3 lipids suggests that there are at least 3 groups of anticardiolipin antibodies.

Antibodies, Anti-Idiotypic↗
PubMed1989

Anticardiolipin antibodies in Behçet's syndrome: a predictor of a more severe disease.

A high incidence of anticardiolipin antibodies were detected in 7 of 20 patients (35%) with Behçet's Syndrome. Three patients had IgG-ab, three had IgM-ab and one had both IgG and IgM antibodies. IgG-ACA was detected mainly in patients with ocular disease (30%) and one of them also has cerebral vascular disease. A lower incidence of ACA was found in the patients taking steroids compared with the ones taking other drugs. This work draws attention to the more severe disease present in patients with ACA and also the possibility of such tests become negative in patients taking immunosuppressive drugs.

Adult↗
PubMed1989

Anticardiolipin antibodies in patients with rheumatoid arthritis.

Anticardiolipin antibodies (ACA) were assayed by ELISA in 73 patients with rheumatoid arthritis. Twelve (16.48%) patients showed levels of ACA three standard deviations above the value of the control group and were considered positive; these patients were compared to the group with ACA within the normal levels regarding the following clinical and laboratorial characteristics: spontaneous abortions, central nervous system involvement, systematization and activity of disease, alterations in platelet counts, presence of antinuclear antibodies and rheumatoid factor. Significant statistical association could be demonstrated between systematization and presence of antinuclear antibodies (ANA) and positiveness to ACA (IgG, IgM or both). These findings might indicate that ACA in patients with RA could have relevance to morbidity of disease or perhaps to its pathogenesis.

Adult↗
PubMed1988

Rheumatoid factor in rabbits with antigen-induced arthritis.

Rheumatoid factor was assayed weekly by hemagglutination in sera from rabbits with antigen-induced arthritis and from control immunized animals receiving intra-articular injections of saline. Both groups developed rheumatoid factor. Arthritic animals, however, maintained high titers of rheumatoid factor more persistently than controls. Pre-incubation of positive sera with native or aggregated rabbit IgG was unable to inhibit agglutination, while native and aggregated human IgG were highly effective. Rheumatoid factor was unable to induce arthritis and probably does not potentiate articular tissue damage by immunocomplexes. Although production of rheumatoid factor may be a consequence of the polyclonal activation promoted by the immunization with complete Freund's adjuvant (CFA), the presence of arthritis is associated with persistence of high titers of rheumatoid factor for longer than would be expected from immunization with CFA alone.

Animals↗
PubMed1988

Antibodies to collagen in experimental antigen induced arthritis of the rabbit.

Fifteen rabbits with antigen (bovine serum albumin) induced arthritis had antibodies to collagens type I, II and III detected weekly by passive hemagglutination. Antitype I collagen antibodies were detected in 80% of the animals in the 3rd week of arthritis; antitypes II and III were found less frequently. No anticollagen antibody was detected after the 6th week of arthritis. Although the appearance of these antibodies was clearly related to the induction of arthritis, results indicate that humoral immunity to collagen is unable to initiate or contribute to the perpetuation of synovitis in this experimental model. Antibodies to collagen are probably an epiphenomenon of articular damage in the antigen induced arthritis of the rabbit.

Animals↗
PubMed1986

Enzyme-linked immunosorbent assay for detection of antibodies to extractable muscle antigens in myasthenia gravis.

A purified citric acid soluble extract from human skeletal muscle (AEMA) and a phosphate-buffered saline extract from rabbit muscle acetone powder (EMA) were used to coat polystyrene beads in an enzyme-linked immunosorbent assay (ELISA). From 54 patients with myasthenia gravis, positive results were observed in 14. Five of 6 patients with thymoma had high levels of antibodies. With the diagnostic difficulties in detecting small and medium-sized thymoma, a sensitive assay for detection of antibodies to muscle antigen may be an important supplementary tool to detect tumors at early stages.

Animals↗
PubMed1982

Carbohydrate cytochemistry on hypodermic lymphatic endothelium of the green lizard, Lacerta hispanica.

A cytochemical study on the endothelium of the hypodermic lymphatic capillaries of the green lizard, Lacerta hispanica, has been carried out. The dialysed iron method produced a homogeneous precipitate on the surface of the endothelial cells and on the inside of the endocytic vesicles. The periodic acid-thiocarbohydrazide-silver proteinate, low pH phosphotungstic acid and high iron diamine techniques gave negative results. The carbohydrates in the capillaries thus seem to be glycosaminoglycans with carboxyl groups. The possible role of these glycosaminoglycans in the formation of the endocytic vesicles is discussed.

Animals↗
PubMed1979

[Multiple clear cell acanthoma in a patient with psoriasis. Treatment with 5-fluorouracil].

Clear cell acanthoma (CCA) is a benign skin tumour, seldom multiple, perfectly individualized both clinically and histologically. The tumour is more usually mistaken for a seborrheic keratosis. There are large amounts of glycogen within the epidermal cells as can see upon staining with the PAS reaction. Melanogenesis is decreased. The enzymes characteristic of eccrine sweat glands are also decreased. The authors report a 67 years old psoriatic female with 15 C.C.A. They emphasize the treatment with topic 5FU particularly interesting in the multiple forms.

Administration, Topical↗