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Biomedical subjects

C Bories

Publications and source records attributed to C Bories.

95 records · Page 6Linked to original sources
PubMedDate not supplied

Marked elevation of serum N-acetyl-beta-D-hexosaminidase activity in rheumatoid rheumatoid arthritis.

OBJECTIVE: To study N-acetyl-beta-D-hexosaminidase (NAHase) activity in the sera of rheumatoid arthritis (RA) patients and to determine its source. METHODS: NAHase activity in the serum and synovial fluid of RA patients was measured with p-nitrophenyl beta-N-acetylglucosaminide as substrate. The p-nitrophenol released was measured spectrophotometrically in an ELISA reader. Rabbit articular chondrocytes in primary culture were stimulated with interleukin-1 beta (IL-1 beta). RESULTS: Serum NAHase activity was higher in 35% of the RA patients than in healthy patients. The median activity was about twice that of the serum of healthy volunteers. RA patients with high serum NAHase activity also had more joint destruction (85%) than those with normal NAHase activity (57%, p < 0.05), but their inflammatory status was similar. The source of NAHase in RA was investigated by assaying it in RA synovial fluids (SF) and measuring its release from articular chondrocytes in primary culture. NAHase activity was detected in all 23 RA SF, at a median concentration that was 2 times that of the serum. NAHase activity in the medium of articular chondrocytes was stimulated by IL-1 beta (p < 0.005 compared to unstimulated cells), suggesting that cartilage is a source of serum and SF NAHase activity. CONCLUSION: The serum concentration of the matrix hydrolase, NAHase, is higher in destructive RA than in inflammatory RA.

Adolescent↗
PubMedDate not supplied

[Use of surviving infectious larvae of Dipetalonema dessetae in study and research on filaricidal substances].

Dipetalonema dessetae in Proechimys oris, the natural final host is a rodent filariasis model used as an in vivo antifilarial screening test. The laboratory vector is Aedes aegypti. Infective larvae L3 isolated from the intermediate host develop and remain healthy for up to 30 days in a biphasic culture medium composed of a cell feeder layer (L 929) and RPMI 1640 supplemented with foetal calf serum. This culture technique has enabled us to screen antifilarial compounds on a new in vitro test. This model has been tested to several pharmacological classes of anthelmintics and effective concentrations 90% are given: diethylcarbamazine 430 mg/l, suramin 490 mg/l, Mel W 3.5 mg/l, mebendazole 78 mg/l, flubendazole 45 mg/l, levamisole 0.55 mg/l, morantel 0.62 mg/l, ivermectin 1.2 mg/l, amoscanate 2.3 mg/l. In vitro test response is remarkable for neurotoxic anthelmintics and nitro-compounds. Furthermore, all compounds considered as reference filaricides are active. For each compound, the in vitro and in vivo results have been compared to appreciate usefulness as well as limits of this in vitro test.

Animals↗
PubMedDate not supplied

[Test tube technique for field diagnosis of hemoglobin S (author's transl)].

Laboratory diagnosis of sickle-cell anemia requires expensive equipment often too sophisticated for rural dispensaries. Therefore haemoglobin precipitation in solutions of neutral salts appears to be a specific and reliable test, as valid as Emmel test, and easily performed during mass campaigns or within the technical and financial limitation of rural medicine.

Adolescent↗