Biomedical subjects

C Bollet

Publications and source records attributed to C Bollet.

60 records · Page 4Linked to original sources

PubMed1984

[Diagnosis of familial medullary cancers].

Medullary thyroid carcinoma (MTC) is hereditary in 20 to 25% of cases. It is inherited as an autosomal dominant trait. MTC can be considered as a sporadic form only after a clinical and biological survey of the two parents, siblings and children of the patient, using pentagastrin stimulation test. The authors have studied 36 patients from 26 families. Hereditary MTC with different clinical features, were discovered in two kindreds. The systematic investigation leads to the discovery of 7 cases in the first family, and of 3 in the second. The treatment of the disease at the first stage of its evolution has been possible when an early diagnosis had been made, such as in the second family.

Calcitonin↗

PubMed1977

[Identification and quantitation of impurities from benorilate (Salipran) by high-performance liquid chromatography (author's transl)].

High-performance liquid chromatography is used for identification and quantitation of impurities which may be encountered in a new antalgic, benorilate (or Salipran), an ester of aspirin with paracetamol. Gradient elution is carried out using a stationary phase consisting of porous 10-micron silica beads bonded to alkylnitrile (Micropak CN), and a mixture of hexane-methylenechloride-methanol-acetic acid with varying methanol percentage as mobile phase. The following impurities were separated from benorilate: acetylsalicylic anhydride, aspirin, acetylsalicylsalicylic acid, salophene, amino-4-phenylacetoxy-2-benzoate, paracetamol, p-aminophenol. The repeatability of the quantitative analysis is good with a standard variation of 0.54% for benorilate (7 injections). Detection is by UV absorption at 254 nm, and detectability is between 2-10(-9) moles for p-aminophenol and 4-10(-11) moles for salophene.

Chromatography, High Pressure Liquid↗

PubMed1976

[High-performance liquid chromatography of penimocycline (author's transl)].

Penimocycline is an antibiotic obtained by Mannich reaction between tetracycline and ampicilline. Separation of penimocycline from tetracyclines and other impurities has been studied by high-performance liquid chromatography. The most effective method is liquid-liquid partition on a Micropak CH column (non-polar hydrocarbon bonded on porous silica microparticles) and gradient elution with water-methanol, 0.02 M phosphate buffer (pH 7.6) and 1 mM EDTA. Some results on hydrolysis of penimocycline are given.

Ampicillin↗

PubMed1976

[Separation by high-performance liquid chromatography of phenylthiohydantoin derivatives of amino acids released by Edman degradation (author's transl)].

The separation by high-performance liquid chromatography of seventeen phenylthiohydantoin derivatives of amino acids released after Edman degradation of proteins is described. Sixteen phenylthiohydantoin derivatives of amino acids are differentiated within 40 min by liquid-liquid partition on a column packed with Micropak CN (moderately polar alkyl nitrile phase bonded on 10mum porous silica microparticles) and gradient elution with hexane=methylene chloride-isopropanol mixtures.

Amino Acids↗

PubMedDate not supplied

[Identification of the principal species of the genus Serratia by a simplified system].

A reduced identification system for the genus Serratia is proposed. The usefulness of this system was shown by identification of 720 strains from clinical specimens or natural environment. Computation of "diagnosis ability coefficient" and principal component analysis made it possible to reduce this identification system to 9 tests. Numerical taxonomy displayed the 9 recognized species.

Enterobacteriaceae Infections↗

PubMedDate not supplied

Phenotypic heterogeneity of Pseudomonas syringae van Hall.

The study of phenotypic properties of 108 strains of Pseudomonas syringae pv. syringae van Hall isolated from Cherry laurel (50 strains) and various host plants (58 strains) and 53 strains of other pathovars of P. syringae and fluorescent Pseudomonas showed that the majority of the strains (91/108) were clustered in one phenon (phenon 14) containing strains commonly considered as P.s. pv. syringae. The present type strain of P.s. pv. syringae was distantly related to phenon 14. Other pathovars of P. syringae constituted 13 discrete phenons.

In Vitro Techniques↗