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C Bollet

Publications and source records attributed to C Bollet.

At least 55 records · Page 3Linked to original sources
PubMed1992

[Extracellular and intracellular activity of sparfloxacin against Mycobacterium avium complex and Mycobacterium xenopi].

Activity of the new fluoroquinolone sparfloxacin against 30 strains of M. avium complex and 25 strains of M. xenopi was tested in vitro. Sparfloxacin was used alone (determination of MICs and MBCs) and in combination with ethambutol and rifabutin. Synergy studies with determination of the FIC and FBC indices showed that the sparfloxacin-ethambutol combination was synergistic against 10 M. avium complex strains and 12 M. xenopi strains. With the three-drug combination (sparfloxacin-ethambutol-rifabutin), synergy was found against 12 M. avium and 14 M. xenopi strains. Studies of intracellular bacteria showed that the decrease in viable bacteria with the three-drug combination was 1 log for M. avium and 2 log for M. xenopi.

Acquired Immunodeficiency Syndrome↗
PubMed1992

Detection and identification of Mycobacterium tuberculosis, M. bovis/BCG, and M. avium by two-step polymerase chain reaction. Comparison with ELISA using A60 antigen.

We propose a rapid two-step PCR to amplify a 767-bp sequence present in the gene coding for the 65-kD antigen of mycobacteria. The high G+C content (80%) permitted annealing to occur at 70 degrees C, enhancing the specificity. The amplified fragment contains a restriction site for differentiation between M. tuberculosis, M. bovis/BCG, and M. avium. Complete diagnosis can be achieved in less than four hours without labelled probe or nucleic acid transfer.

Antigens, Bacterial↗
PubMed1992

Molecular cloning of a specific DNA probe for the identification of Bacillus licheniformis.

Bacillus licheniformis has been found to be one of the dominant nosocomial species of Bacillus: laboratories dealing with nosocomial infections must be able to identify Bacillus up to the species level. To date, no DNA probes have been isolated for B. licheniformis although there is a clear need for a direct detection by polymerase chain reaction. The isolation of a B. licheniformis-specific DNA probe, as described in this paper, represents the first step toward accomplishing this goal.

Bacillus↗
PubMed1991

Isolation of Enterobacter amnigenus from a heart transplant recipient.

The isolation of Enterobacter amnigenus from a human source is reported. This microorganism was grown from an intravenous catheter and, along with Pseudomonas aeruginosa, from the blood of a heart transplant patient. The biochemical characteristics and antimicrobial susceptibilities of the isolate are described.

Bacteremia↗
PubMed1991

[Activity of antibiotics against pigmented and unpigmented variants of Mycobacterium avium-intracellulare].

Efficacy of various antibiotics: amikacin, rifampicin, rifabutin, ciprofloxacin, temafloxacin, erythromycin and clarithromycin was evaluated against pigmented and unpigmented variants of Mycobacterium avium complex isolated from patients with acquired immunodeficiency syndrome. The minimal inhibitory concentrations of antibiotics against unpigmented variants were multiplied by 2 to 16 compared to those of pigmented variants. Observations with intracellularly growing bacteria showed that unpigmented variants were more resistant to antibiotics than variants with a difference of approximately 0.25 a 0.3 log 10 in decrease of viable or bacterial counts. The combinations of temafloxacin or ciprofloxacin with rifabutin and amikacin were most effective against unpigmented variants.

Acquired Immunodeficiency Syndrome↗
PubMed1991

[Intra-macrophagic activity of antibiotics combinations against Mycobacterium marinum].

Fourteen strains of Mycobacterium marinum were isolated from patients with granulomatous skin lesion. All the strains were resistant to isoniazid and pyrazinamid and five resistant to rifampicin. The following antibiotics are used alone or in combination: ethambutol, rifabutin, ciprofloxacin, temafloxacin, erythromycin, clarithromycin, minocyclin. Minimal inhibitory concentrations are evaluated using agar dilution method. After what, activity of antibiotics against Mycobacterium marinum strains within human macrophages in investigated. After treating the monolayers at 24 and 96 h, a bactericidal effect is observed with rifabutin, ciprofloxacin, temafloxacin, clarithromycin and minocyclin. Combinations of ciprofloxacin, temafloxacin or clarithromycin with ethambutol and rifabutin produced synergistic effect.

Anti-Infective Agents↗
PubMed1991

[Pulmonary actinomycosis. Apropos of a case].

The authors report a case of pseudo-tumoral thoracic actinomycosis with lysis of a rib in a young man who was a heavy smoker and drinker. This observation is an opportunity to review the usual difficulties of diagnosis in patients with this rare disease occurring most often in a patient who is debilitated with important disease in the teeth and gingival margins. The standard treatment is penicillin G, which leads to a cure within one month.

Actinomycosis↗
PubMed1990

[Isolation, morphological and biochemical characteristics of gram-positive bacteria metabolizing beta-caryophyllene].

Eleven strains of coryneform bacteria were isolated from soil samples by enrichment culture in a mineral medium containing beta-caryophyllene as the sole energy and carbon source. Ten of them could also metabolize longifolene. Numerical taxonomy, based on the use of 147 characteristics, revealed a large diversity. The DNA G + C content was found to be in the range of 62.5-68.3 mol%. Three strains were placed in "The National Collection of Industrial and Marine Bacteria".

Actinomycetales↗
PubMed1990

[Activity of azithromycin and roxithromycin alone or in combination against Mycobacterium avium and Mycobacterium xenopi].

The effect of two new macrolides, azithromycin and roxithromycin used either alone or in combination with amikacin rifabutine and 1.25 (HO)2 vitamin D3 was examined in vitro. Macrophage monolayers infected with M. avium complex or M. xenopi were treated with antibiotics or 1.25 (OH)2 vitamin D3 by using different protocols: antibiotics or 1.25 (OH)2 vitamin D3 was added to the macrophage monolayers immediately after infection and released by washing out after 24 h; antibiotics or 1.25 (OH)2 vitamin D3 was replenished daily for 4 days; or infected macrophage monolayers were treated with antibiotics plus vitamin D3 for 4 consecutive days. Treatment for 24 h resulted in an inhibition of growth. After treating the macrophage monolayers with the four antibiotics alone or in combination for 4 consecutive days intracellular killing of mycobacteria was observed and the combinations were significantly more lethal than antibiotics alone. The mycobactericidal effect was enhanced when vitamin D3 was added to the culture.

Azithromycin↗
PubMed1990

[Post-antibiotic effect of imipenem, amikacin and ciprofloxacin against various strains of Serratia marcescens].

The authors compared the post-antibiotic effect (PAE) of imipenem, amikacin, ciprofloxacin, and latamoxef against Serratia marcescens ATCC 13880 (type strain) and against 12 clinical strains belonging to Grimont's most frequent biotypes: A2a, A3a, A3b, A4a, A4b, A5, A6a, A8a, A8b, A8c, TT, TCT. PAE was determined by measuring bacterial growth kinetics after one hour exposure to concentration of 2 x MIC of 10(6) CFUs in Mueller-Hinton broth. Drug removal was by 10-3 dilution of the exposed culture. A PAE was consistently present with imipenem (range 0.8-2.9 hrs), amikacin (range 1.0-4.9 hrs), ciprofloxacin (range 1.4-2.8 hrs). The duration of PAE did not correlate with MIC or Grimont's biotypes.

Amikacin↗
PubMed1990

[Identification of Campylobacter jejuni by using a biotinylated probe].

A DNA biotinylated probe for the rapid identification of Campylobacter jejuni in culture (Enzo Biochem, New York) has been evaluated. The hybridized biotinylated DNA probe is detected by its interaction with streptavidin linked to horseradish peroxidase. Sixteen strains of C. jejuni, including type strain. 24 strains of other Campylobacter and Helicobacter species, and 59 strains of other general have been studied. The probe was highly sensitive (100%) and specific (100% inside the genera Campylobacter and Helicobacter). All Campylobacter strains gave strong signals, and only three weak signals have been observed with non-Campylobacter strains. Our results indicate that specific recombinant DNA probe should offer a reliable and rapid method for routine diagnosis of Campylobacter jejuni enteritis.

Campylobacter fetus↗
PubMed1989

[Evaluation of a biotinylated probe for identification of Neisseria gonorrhoeae].

We evaluated a DNA biotinylated probe for the rapid identification of Neisseria gonorrhoeae in culture (Ortho Diagnostics System, Raritan, NJ). Twenty-one strains of N. gonorrhoeae, including type strain, 57 strains of other Neisseria species, and 104 strains of other genera were studied. The probe was highly sensitive (100%) and specific (96%). All N. gonorrhoeae strains gave strong signals, and only two cross-reaction were observed with N. lactamica, which has a close genetic relationship to N. gonorrhoeae. Our results indicate that specific recombinant DNA probe should offer a reliable and rapid method for routine diagnosis of Neisseria gonorrhoeae.

Biotin↗
PubMed1989

[Bacteriostatic and bactericidal study of rifabutine and clofazimine in combination against Mycobacterium avium-intracellulare and Mycobacterium xenopi].

The authors have tested the sensibility to rifabutine and clofazimine of seventeen strains of M. avium and eleven strains of M. xenopi. They compared the ability of these and other antimicrobial agents ciprofloxacin, amikacin and erythromycin alone or in combinations to inhibit the growth of strains of the M. avium complex and of M. xenopi. The purpose of this investigation was also to examine the bactericidal action of these antimicrobial agents and to determine the ability of combinations to kill strains of M. avium and M. xenopi.

Amikacin↗
PubMed1988

[Identification of coagulase-negative Staphylococci. Significance of miniaturized systems].

To evaluate accuracy of API Staph. and Staph. Ident. systems for identification of coagulase-negative Staphylococci, the authors have tested 235 clinical and environmental isolates. The results obtained were compared with those of conventional method of Kloos and Schleifer. API Staph. and Staph. Ident. correctly identified respectively 64 and 53 p. cent of the strains. Diagnosis ability coefficient (Descamps and Véron) are close. It should be necessary to compare identification obtained using analytical profile index with described characteristics of species.

Bacteriological Techniques↗
PubMed1988

[Application of the diagnostic ability coefficient to the comparison of 3 identification miniaturized systems for identifying anaerobic bacteria].

The authors compare the performances of three identification systems for anaerobic bacteria. The computation of diagnosis ability coefficient (Descamps and Véron) made it possible to classify these systems to identify a batch of eighty strains coming from pathologic samples. The results are very close. This study shows profile catalogues' short coming and the disadvantages of their use which appears to be insufficiently critical. Every-day practice of these supplementary tests and the use of gas-liquid chromatography seem necessary to identify completely the anaerobic bacteria of the studied batch.

Bacteria, Anaerobic↗