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Biomedical subjects

C Barriga

Publications and source records attributed to C Barriga.

At least 73 records · Page 4Linked to original sources
PubMed1992

Effect of aztreonam upon human polymorphonuclear leukocyte functions.

Aztreonam (SQ 26,776), a new synthetic monobactam with an excellent antibacterial spectrum was analyzed in vitro. In this paper, the effect of aztreonam on the phagocytic process of human neutrophils isolated from fifteen healthy volunteers was studied. Chemotaxis was not modified with aztreonam at any of the doses used (10, 20, 40, 60, 80 and 100 micrograms/ml). On the other hand, this antibiotic at 100 micrograms/ml significantly increases (P less than 0.05) Candida albicans ingestion as well as their digestion by neutrophils at 10 and 100 micrograms/ml.

Aztreonam↗
PubMed1992

Phagocytosis of latex beads by alveolar macrophages from mice exposed to cigarette smoke.

Cigarette smoking is known to alter the numerical presence and function of alveolar macrophages. It has been speculated that these cigarette-smoke-induced alterations contribute to the depressed pulmonary defence mechanism commonly demonstrated in smokers. Studies of the phagocytic and bactericidal activities of alveolar macrophages from smokers and non-smokers have yielded conflicting results. The purpose of this study was to investigate the phagocytic capacity of alveolar macrophages from mice exposed to cigarette smoke in relation to the ability to ingest inert particles (latex beads). Measurements were made before (basal values), immediately after, and 1, 12 or 24 h after exposure. Significant decreases were observed in the number of latex beads ingested by 100 macrophages (phagocytic index) and in the phagocytic efficiency for ingesting latex (mean number of latex beads ingested per activated macrophages) immediately after and 1 h after exposure, and in the number of activated macrophages (those with phagocytic activity) immediately after exposure.

Animals↗
PubMed1991

Phagocytic function of blood neutrophils in sedentary young people after physical exercise.

The effect of exhaustive running exercise on the phagocytic function of blood polymorphonuclear neutrophils in sedentary young men and women has been studied. Adherence capacity to the endothelium, spontaneous mobility, chemotaxis and ingestion of Candida albicans were not modified after physical exercise. However, opsonization of Candida albicans as well as candidicide power increased significantly in men and women after exercise. The immediate advantages of physical exercise on the phagocytic immune response is discussed.

Adult↗
PubMed1991

Stimulation of phagocytic processes and antibody-dependent cellular cytotoxicity of human neutrophils by cefmetazole.

Interactions between antimicrobial agents and phagocytic cells, especially neutrophils, have a potential role in the treatment of infections. The in vitro effects of cefmetazole, a novel beta-lactam antibiotic, at a therapeutic concentration reached in plasma (50 micrograms/ml) on phagocytic and cytotoxic functions of human neutrophils have been studied. In human neutrophils, adherence capacity to nylon fiber and to substrate, chemotaxis, attachment to and ingestion of Candida albicans (with serum, with decomplemented serum and without serum), ingestion of inert particles (latex beads), candidicidal activity and superoxide anion production were all stimulated by cefmetazole. Cefmetazole at this dose was a chemotactic agent for neutrophils. Antibody-dependent cellular cytotoxicity (ADCC) was also increased by this anti-microbial agent.

Antibody-Dependent Cell Cytotoxicity↗
PubMed1990

Phagocytic function and antibody-dependent cellular cytotoxicity of human neutrophils in the presence of N-formimidoyl thienamycin.

The efficacy of an antibiotic in the treatment of bacterial infections depends upon the interactions of the drug, bacteria and phagocytes. We have studied "in vitro" the effect of N-formimidoyl thienamycin (Imipenem), a novel beta-lactamic antibiotic, on the phagocytic function and antibody-dependent cellular cytotoxicity of human neutrophil leukocytes. The incubation of these cells with 50 micrograms/ml of Imipenem similar to the therapeutic levels reached in plasma results in an increase of their adherence capacity to nylon fiber and to substrate, induced mobility or chemotaxis, opsonization, phagocytosis of Candida albicans (with serum, with decomplementarized serum and without serum) and latex beads, candidicidal power and the capacity of NBT reduction. Imipenem at this dose also presents chemoattractant power for neutrophils and enhances the antibody-dependent cellular cytotoxicity (ADCC).

Antibody-Dependent Cell Cytotoxicity↗
PubMed1990

Changes in the number of leukocytes and lymphocyte subpopulations induced by exercise in sedentary young people.

The influence of physical exercise on the quantification of leukocytes and lymphocyte subpopulations has been studied. These cells were obtained from blood taken from eleven healthy young men and women who follow a sedentary life style, before and after vigorous exercise (running). The results indicate that physical activity increases the number of white blood cells and the concentrations of circulating lymphocytes. The proportion of T-lymphocytes, estimated as rosette forming cells with sheep red blood cells after cold incubation, is constant, whereas a corresponding increase in cells with receptors for C3b or Ig-Fc is also observed. The data indicate that physical activity leads to an irregular intravascular discharge in stored cells.

Adult↗
PubMed1989

Effects of acetylsalicylic acid on the phagocytic function of human polymorphonuclear leukocytes in vitro.

1. In this paper acetylsalicylic acid (aspirin), an anti inflammatory drug, was studied in vitro at doses of 50, 100, 200 and 500 mg/l to see its effects on adherence, chemotaxis, spontaneous mobility, phagocytosis, candidicide power, nitrobule tetrazolium (NBT) reduction as well as the incorporation and metabolism of arachidonic acid in human polymorphonuclear neutrophils (PMNns). 2. Aspirin significantly stimulated neutrophil adherence to nylon fiber at all the doses used, with a correlation between the doses used and the adherence indices found. 3. At the therapeutic dose (100 mg/l) aspirin brings about a significant increase of chemotaxis, but reduces this property at the highest dose (500 mg/l). On the other hand, spontaneous mobility is not altered except with the 500 mg/l dose of aspirin which produces a significative decrease. 4. The ingestion of Cándida albicans by PMNns is significant at the therapeutic dose; the candidicide power is not modified with any of the doses used with 100 mg/l of aspirin nitroblue tetrazolium (NBT) reduction is significantly increased. 5. No changes are observed in the incorporation of arachidonic acid or in the release of its metabolites.

Arachidonic Acid↗
PubMed1989

In vitro and in vivo effects of Imipenem on phagocytic activity of murine peritoneal macrophages.

Imipenem, a new antibiotic beta-lactam, and Tienam (an Imipenem/Cilastatine combination) have been studied in vitro and in vivo respectively, in the phagocytic function of macrophages. In this paper we have seen the variations produced by 50 mg/l of Imipenem and 120 mg/kg of Tienam in the adherence, spontaneous mobility, chemotaxis, opsonization, phagocytosis of Candida albicans and latex beads, candidicid effect and nitroblue tetrazolium (NBT) reduction in peritoneal macrophages from BALB/C mice. This antibiotic significantly increases in vitro and in vivo the adherence, spontaneous mobility and chemotaxis, phagocytosis of latex beads and the digestion of ingested material (nitroblue tetrazolium reduction) in the above-mentioned cells. The number of Candida albicans opsonized and ingested by the macrophages is not modified in the presence of Imipenem, and neither is the candidicid effect.

Animals↗
PubMed1988

[Effect of calcium, glucose and trifluoroperazine on the carboxymethylation of proteins in pancreatic islets of rats].

In the isolated pancreatic islets incubated in vitro, carboxylmethylation increases following stimulation with 20 mM glucose. This increase is dependent on extracellular calcium concentration and is suppressed in the presence of trifluoperazine, a calmodulin inhibitor. In conclusion, the increase in carboxylmethylation induced by 20 mM glucose seems to be a calcium-dependent process and it is probably mediated by calmodulin.

Animals↗
PubMed1987

Effects of cefmetazol, cefoxitin and imipenem on polymorphonuclear leukocytes.

1. We have investigated the effects produced in vitro by Cefmetazol, Cefoxitin and Imipenem on the chemotaxis, spontaneous mobility, adherence, phagocytosis and candidicide power of human polymorphonuclear neutrophils (PMNs). 2. The three antibiotics tested significantly stimulated the adherence of neutrophils to nylon fibre at doses either equal (50 mg/l) or superior (500 mg/l) to the therapeutic one. 3. Cefmetazol, Cefoxitin and Imipenem bring about a maximum increase of chemotaxis at the therapeutic dose, whereas the spontaneous mobility diminishes with any one of the doses used. 4. The capacity of the PMNs to phagocytize and produce lysis of Candida albicans is increased in Cefmetazol with therapeutic doses. Cefoxitin produced an increased lysis of candidas.

Candida albicans↗
PubMed1985

Red cell choline in Korsakoff psychosis.

Red blood cell (RBC) choline was measured in 48 male patients with Korsakoff psychosis secondary to alcoholism and in 19 age-matched male controls using gas chromatography-mass spectrometry. The variance in RBC choline was significantly different between Korsakoff patients and controls (p less than 0.002). RBC choline was significantly higher in Korsakoff patients compared with controls (p less than 0.02). There was no significant correlation between age and RBC choline. Higher RBC choline levels suggest differences in membrane transport properties. Differences in membrane properties may indicate a neuronal membrane vulnerability to the toxic effect of alcohol or to thiamine deficiency which lead to the degenerative changes associated with Korsakoff psychosis.

Aged↗
PubMed1981

A study of some pharmacological effects of a new synthetic C-nucleoside.

The effect of a new synthetic C-nucleoside (2-beta-D-lyxopyranosyl-6,6-dimethyl-4,5,6,7-tetrahydroindol-4-one) on cell differentiation and growth was studied through three kinds of experiments: a) Appearance rate of peripheral blood cells in AKR mice, b) Growth capacity of Ehrlich ascite tumor cells and c) Reappearance rate of membrane receptors on T-lymphocytes which from E-rosette aggregates with sheep red cells. This C-nucleoside produced an inhibitory effect on all the processes studied, although not so strong as with the reference drug (methotrexate).

Animals↗