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Biomedical subjects

C B Taylor

Publications and source records attributed to C B Taylor.

At least 235 records · Page 13Linked to original sources
PubMed1975

Enolase isoenzymes. II. Hybridization studies, developmental and phylogenetic aspects.

1. Hybridization studies have been carried out in vitro using mixtures of partially purified isoenzymes 1 and 3 of rat enolase (2-phospho-D-glycerate hydrolyase, EC 4.1.1.11). Immunological methods were used to demonstrate the formation of the hybrid. 2. Immunological analysis of the elution peaks from QAE-Sephadex chromatography of heart enolase indicates the occurrence in vivo of the hybrid enolase 2. 3. Developmental changes in the proportions of isoenzymes 1, 2 and 3 in heart and skeletal muscle of rat have been studied quantitatively. In both tissues isoenzyme 1 predominates in the foetus, but is partially replaced by 2 and 3 in adult heart and completely by 3 in the adult muscle. 4. Evidence is given of the binomial distribution of the proportions of the three isoenzymes in the developing heart. 5. Phylogenetic studies of the immunological properties of enolases from muscle, liver and heart have been carried out. 6. It is concluded that the three isoenzymes arise from two independent genetic loci and it is suggested that these evolved from a common ancestral gene 200-300 million years ago.

Aging↗
PubMed1975

Using rumors for therapeutic purposes.

Mental health workers and programs in small towns become the subject of rumors. A method is suggested for the investigation of such rumors and their use for therapeutic purposes. Three rumor case histories are presented to illustrate these ideas. The function of rumors in a small town is discussed.

Community Mental Health Services↗
PubMed1975

Carbohydrate and lipid metabolism in the Alaskan Arctic Eskimo.

The effect of a low total carbohydrate low sucrose diet on various parameters of lipid and carbohydrate metabolism was studied among residents of a North Slope Alaskan Eskimo village. For comparative purposes a group of Eskimo youths consuming a higher carbohydrate institutional diet was also stidied. Those Eskimos consuming their native diet had unusually low serum triglycerides and very low density lipoproteins and normal glucose and tolbutamide tolerance tests. Eskimos on a higher carbohydrate diet exhibited significant elevations of triglycerides and minor alterations in glucose tolerance testing. Differences in cholesterol intake between these groups were accompanied by changes in serum cholesterol and low density lipoprotein levels. Unusually high levels of free fatty acids without ketonemia in the North Slope sample were noted as well.

Adolescent↗
PubMed1973

Relationships between concentration of hepatic intermediary metabolites and induction of the key glycolytic enzymes in vivo.

1. The time-course for the induction of hepatic glucokinase, hexokinase, phosphofructokinase, liver-type and muscle-type pyruvate kinases in reponse to various diets and insulin has been investigated over the first 48h of change in both diabetic and non-diabetic rats. 2. The results are consistent with there being separate regulatory mechanisms for the induction of each of the three key enzymes, that is for glucokinase, phosphofructokinase and liver-type pyruvate kinase. 3. To investigate the possibility that induction of these enzymes is mediated through specific metabolites a full metabolite profile has been determined under conditions identical with those in the induction experiments and the results examined for correlations between metabolite concentrations and enzyme activities. 4. Several such relationships were detected and those between glucokinase activity and the phosphorylation state of the adenine nucleotides and between liver-type pyruvate kinase activity and the concentrations of dihydroxyacetone phosphate and pyruvate are discussed in relation to the concept of inducing metabolites. 5. It is suggested that the induction of glycolytic enzymes by insulin may be secondary to the changes in the concentration of specific hepatic metabolites brought about by the acute effects of the hormone. 6. The details of the metabolite concentrations in the various experimental states have been deposited as Supplementary Publication SUP 50021 at the British Library (Lending Division) (formerly the National Lending Library for Science and Technology), Boston Spa, Yorks. LS23 7BQ, U.K., from whom copies can be obtained on the terms indicated in Biochem. J. (1973), 131, 5.

Animals↗