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Biomedical subjects

C B Rodning

Publications and source records attributed to C B Rodning.

49 records · Page 3Linked to original sources
PubMed1983

Light microscopic morphometric analysis of rat ileal mucosa. I. Component quantitation of IgA-containing immunocytes.

A light microscopic morphometric analysis of IgA-containing immunocytes within samples of ileal mucosa was performed. The following groups of rats were studied: (1) animals raised in a gnotobiotic environment (microbial reduction); (2) animals with iatrogenic self-filling intestinal blind loops (microbial proliferation); and (3) control animals (sham operation). The unlabeled antibody enzyme immunohistochemical localization technique was employed for the identification of intracellular IgA. Component quantitation involved use of a micrometer component quantitator. Numerical density of the immunocyte population was determined by component quantitation of individual and total immunocyte volumes and by application of the Floderus equation. The methodology employed provided a precise quantitative analysis of all mucosal components of normal and manipulated rat ileum. A statistically significant reduction in the volume percentage of IgA-containing immunocytes in association with both microbial reduction and microbial proliferation was observed. The volume percentage reduction of the IgA-containing immunocyte population associated with gnotobiosis may reflect decreased microbial antigenic stimulation, whereas that associated with microbial proliferation may reflect the presence of an increased population of immunocytes producing non-IgA immunoglobulins.

Animals↗
PubMed1983

Colostomy closure. Morbidity reduction employing a semi-standardized protocol.

To evaluate a semi-standardized protocol for colostomy closure, the cases of 166 consecutive patients from 1974 through 1981 were analyzed retrospectively. There were 17 complications (17/166); overall morbidity rate was 2.4 per cent. A significantly increased incidence of major morbidity and septic complications was associated with colostomies closed at an interval of less than 8.5 weeks from formation (P less than or equal to 0.001). Simple transverse closure of colostomy versus resection and end-to-end anastomosis did not result in increased morbidity (P less than or equal to 0.1). The wound infection rate was 1.2 per cent (2/166) with 135/166 wounds closed primarily, or primarily over a subcutaneous drain, thus rendering primary wound closure safe and desirable.

Adolescent↗
PubMed1983

Disaster preparedness.

The only rational approach to the potential chaos of a mass disaster is thoughtful predisaster preparation and realistic but optimistic confrontation of the situation. An appropriate response implies accurate assessment of the magnitude of the disaster, the establishment of a reliable and effective communication system, a realistic inventory of and provision for sustained personnel and logistical support, and proper triage of individual casualties. It is incumbent upon the medical community to provide imaginative and creative responses to the concept of mass casualty management, and for all physicians involved to function in the generic sense as trauma surgeons.

Disaster Planning↗
PubMed1982

Light microscopic morphometric analysis of rat ileal mucosa: II. Component quantitation of Paneth cells.

A quantitative light microscopic morphometric analysis of lysozyme- and IgA-containing Paneth cells within the ileal mucosa of physiologically manipulated and control (sham operation) animals was performed. The experimental groups of rats included animals raised in a gnotobiotic environment (microbial reduction) and animals with ileal self-filling blind (microbial proliferation) and Thiry-Vella (intestinal discontinuity) loops. The unlabeled antibody enzyme immunohistochemical localization technique was employed for the identification of intracellular lysozyme and IgA. Component quantitation was performed by use of a micrometer component quantitator. Marked Paneth cell hyperplasia was noted in association with gnotobiosis and with the Thiry-Vella fistula. This observation quantitatively confirms previous subjective impressions of increased Paneth cell differentiation in association with those physiologic states. Since the neurovascular component of the Thiry-Vella fistula is intact, the normal intraluminal succus entericus would appear to be involved in modulation of Paneth cell differentiation. The recognition of Paneth cell hyperplasia in association with the Thiry-Vella fistula suggests that this may be a useful experimental model for an evaluation of the life cycle and functional characteristics of this cell population. The results also revealed that no significant change in the volume percentage of Paneth cells and a decreased volume percentage of Paneth cells containing IgA occurred in association with the self-filling blind loop. A decreased volume percentage of IgA-containing immunocytes in association with the blind loop has previously been reported. The data are most consistent with the interpretation that the Paneth cell and immunocyte response to antigenic stimulation are interrelated and that the Paneth cell population has a restricted latitude of response to microbial proliferation.

Animals↗
PubMed1982

A quantitative morphometric analysis of rat ileal Thiry-Vella fistulae.

Controversy exists regarding the mechanism and degree of mucosal alterations occurring in defunctionalized segments of intestine. This study compares the results of a quantitative analysis of mucosal components, including Paneth cells and immunocytes, between in-continuity and defunctionalized (Thiry-Vella) segments of rat ileum. The micrometer component quantitator was used for the light microscopic morphometric analysis. Intracellular lysozyme and IgA were identified employing the unlabeled antibody enzyme immunohistochemical staining technique. The vol% of the ileal mucosal components of animals from the control group and from the in-continuity segments of the experimental group were comparable. Analysis of the Thiry-Vella fistulae, however, revealed a statistically significant decrease in the vol% of columnar epithelial cells and increase in the vol% of lysozyme-containing Paneth cells and interstitium of the lamina propria. Since Thiry-Vella fistulae are neurovascularly intact, mucosal alterations imply a causal relationship to interaction with chyme. The data suggest that chyme has both a stimulatory (on the columnar epithelium) and suppressive (on the the Paneth cell population) effect. The vol% of IgA-containing Paneth cells and the percentage of the lamina propria represented by IgA-containing immunocytes were also substantially decreased. Normally secretory IgA is the immunoglobulin of highest concentration intraluminally and among immunocytes within the lamina propria, presumably in response to local antigenic stimulation. The presence of immunoglobulin within Paneth cells may reflect the phagocytosis of immunoglobulin complexed antigens. The data suggest that the degree of local antigenic stimulation is decreased in Thiry-Vella fistulae.

Animals↗
PubMed1980

Immunohistochemical identification of immunoglobulin A on ultrathin tissue sections.

Antigenic determinants of the heavy chain of immunoglobulin A (IgA) were identified within immunocytes by immunohistochemical techniques applied to ultrathin sections of rat ileal mucosa embedded in eposy resin. Successful localization of IgA depended upon the utilization of chemical fixatives containing paraformaldehyde or physical fixation employing a freeze-drying technique. Partial removal of the epoxy resin from the ultrathin tissue section with metallic alkoxide solutions or pretreatment with H2O2 was essential prior to the sequential application of the immunological reagents. IgA was associated with the rough endoplasmic reticulum (RER) and nuclear regions of immunocytes. These results are similar to those of other investigators employing immunochemical localization before embedment.

Animals↗
PubMed1976

Immunoglobulins within human small-intestinal Paneth cells.

Human duodenal, jejunal, and ileal samples obtained at necropsy and by peroral and surgical biopsy, were studied by light microscopy using the unlabelled antibody enzyme method for imunocytochemical staining of lysozyme and immunoglobulins. Paneth cells contained IgA and IgG, but not IgD IgE, or IgM. Staining intensity indicated that IgA and IgG were present in amounts greater than in other epithelial cells. There was pronounced variation in the immunoglobulin content of Paneth cells. Rat Paneth cell containing IgA and lysozyme and are capable of the phagocytosis and degradation of microorganisms. These observations suggest that human Paneth cells may have similar functional capabilities.

Duodenum↗
PubMed1976

Immunocytochemical identification and localization of immunoglobulin A within Paneth cells of the rat small intestine.

Light microscopic immunocytochemistry was used to identify Paneth cells by their lysozyme content and to detect immunoglobulin antigens within a subpopulation of these cells. Antisera specific for the heavy chains of rat or human immunoglobulin A and for immunoglobulin light chain antigens produced specific staining of rat Paneth cells. The distribution of immunoglobulin staining varied between adjacent Paneth cells in the same crypt and between Paneth cells in adjacent crypts, as well as between Paneth cell populations of different animals. No staining of rat Paneth cells was detected using antisera specific for the heavy chain of immunoglobulins G or M. The specific staining of Paneth cells for immunoglobulin A and light chain antigens was blocked by absorption of each antiserum with its respective purified antigen. Absorption of these antisera with purified rat lysozyme did not affect staining and thereby eliminated the possibility of immunologic cross-reactivity between lysozyme and immunoglobulin antigens. It is suggested, in light of current concepts of Paneth cell function, that the immunoglobulin staining of Paneth cells may reflect their ability to phagocytize immunoglobulin A-coated microorganisms or immune complexes containing immunoglobulin A.

Animals↗
PubMedDate not supplied

A surgical diary: concepts and tenets.

A surgical diary may be one of the most utilitarian mechanisms for acquiring, recalling, and reinforcing surgical knowledge and skills. A surgical diary facilitates capture of the subtlety of each operation as provided by a diarist's preceptors, incorporating and utilizing what is valid and rejecting and discarding what is otherwise. Use of a surgical diary could be an effective learning instrument.

General Surgery↗