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Biomedical subjects

C A Taylor

Publications and source records attributed to C A Taylor.

At least 91 records · Page 5Linked to original sources
PubMed1982

Some physicochemical characteristics of photoaffinity-labeled rabbit androgen-binding protein.

Photoaffinity-labeled androgen-binding protein (ABP), present in cytosol prepared from the caput epididymidis of intact sexually mature rabbits, was produced using 17 beta-hydroxy-[1,2-3H]4,6-androstadien-3-one (delta 6-testosterone). Photo-labeled ABP could not be distinguished from unlabeled ABP by gel filtration chromatography, electrophoresis on nondenaturing polyacrylamide gels, or sucrose gradient ultracentrifugation. Rabbit ABP was found to have a sedimentation coefficient of 4.6S and a Stokes radium of approximately 45A. Based on these parameters, its native molecular weight was calculated to be approximately 86,300. A model of ABP as a prolate ellipsoid having an axial ratio of 10 is consistent with its frictional ratio of 1.555. When photolabeled rabbit ABP was examined on polyacrylamide gels containing sodium dodecyl sulfate, two androgen-specific peaks of approximately 52,000 and approximately 48,000 daltons were detected. Both peaks contained approximately the same amount of radioactivity. When photolabeled ABP was treated with the cross-linking reagent disuccinimidyl suberate before electrophoresis on gels containing sodium dodecyl sulfate, an additional androgen-specific peak corresponding to approximately 100,000 daltons was obtained. We interpret this peak to represent dimers of the lower molecular weight species. The 52,000- and 48,000-dalton subunits were observed regardless of whether the proteins were treated with 2-mercaptoethanol, indicating that the monomers are not linked by disulfide bonds.

Affinity Labels↗
PubMed1982

Some physicochemical characteristics of photoaffinity-labeled rabbit testosterone-binding globulin.

Photolabeled testosterone-binding globulin (TeBG), obtained from the plasma of sexually immature male rabbits, was produced using 17 beta-hydroxy-[1,2-3H]4,6-androstadien-3-one. Photolabeled TeBG could not be distinguished from unlabeled TeBG by gel filtration chromatography, electrophoresis on nondenaturing gels, or sucrose gradient ultracentrifugation. Rabbit TeBG had a Stokes radius of approximately 45 A and a sedimentation coefficient of approximately 4.6S. Based on these parameters, its native molecular weight was calculated to be approximately 78,000. The frictional ratio of rabbit TeBG was found to be approximately 1.6. When photolabeled TeBG was examined on polyacrylamide gels containing sodium dodecyl sulfate, a single androgen-specific peak of approximately 40,000 daltons was obtained. When photolabeled TeBG was treated with the cross-linking reagent disuccinimidyl suberate before electrophoresis on sodium dodecyl sulfate gels, an additional androgen-specific peak of radioactivity corresponding to approximately 100,000 daltons was obtained. We interpret this peak to represent oligomers of the TeBG subunits. The 40,000-dalton subunit was obtained regardless of whether the proteins were treated with 2-mercaptoethanol, indicating that the monomers are not linked by disulfide bonds.

Affinity Labels↗
PubMed1982

Reactions of families to the death of a child with congenital heart disease.

We interviewed 57 parents of 33 children who died of congenital heart disease to obtain their descriptions and perceptions of their own grief. Irrespective of the child's age or length of illness, the parents consistently described a grief reaction characterized by an initial period of shock lasting hours to several weeks which evolved into a four- to six-month period of sadness/depression with somatic complaints, which slowly progressed to resolution through acceptance. No parent described symptoms of pathologic grief. Only four of 33 surviving siblings demonstrated recognizable problems at the time of follow-up. There was physician follow-up of 15 families; only two families received follow-up from local physicians, even though nine children died at home or in community hospitals. All parents expected follow-up and accepted it when offered. Specific follow-up recommendations are made.

Adolescent↗
PubMed1981

The King syndrome: malignant hyperthermia, myopathy, and multiple anomalies.

Heterogeneity in malignant hyperthermia is being recognized with increasing frequency. In 1973, King described a subgroup of malignant hyperthermia patients with slowly progressive myopathy, short stature, kyphoscoliosis, pectus carinatum, cryptorchidism, and a characteristic facial appearance. Seven cases have been reported, all in males. We present the case of a girl with similar clinical findings who died in a malignant hyperthermia crisis and review the previously reported cases of King syndrome. The delineation of King syndrome provides further evidence for the heterogeneity of malignant hyperthermia and increases awareness of this disorder; its recognition in a female may lead to preoperative diagnosis and prevention of malignant hyperthermia crisis in King syndrome patients.

Abnormalities, Multiple↗
PubMed1981

Insulin degradation. XXVIII. Immunocytochemical localization of glutathione-insulin transhydrogenase in the pancreas, kidney and liver of normal and streptozotocin-diabetic rats and of lean and obese (ob/ob) mice.

Glutathione-insulin transhydrogenase catalyzed the inactivation of insulin by splitting the hormone into A and B chains. We have localized this enzyme immunocytochemically by light microscopy in the pancreas, kidney and liver of both lean and obese (ob/ob) mice and similarly in normal and streptozotocin-diabetic rats. Localization was achieved by an antibody to glutathione-insulin transhydrogenase using a peroxidase-antiperoxidase technique. In comparison with tissues from control animals, positive immunostaining for glutathione-insulin transhydrogenase was increased in the obese mouse but reduced in the diabetic rat. Different tissues showed considerable variation in the amount of glutathione-insulin transhydrogenase which could be detected. In the pancreatic islets there was little or no evidence for the presence of the enzyme in peripheral cells. In the kidney, immunocytochemical staining was found only in the proximal tubules. In the liver there was a generalised distribution of the enzyme, but the greatest concentration was in the periportal region. These observations parallel the biochemical data relating to glutathione-insulin transhydrogenase, indicating that different amount of insulin degrading activity exist in different regions of tissues.

Animals↗
PubMed1981

(alpha S)-erythro-alpha-methylepinephrine: preparation and stereoselective binding to adrenergic receptors in rat forebrain.

The enantiomers of a number of catecholamines, including (alpha S)- and (alpha R)-erythro-alpha-methylepinephrine, were evaluated for their capacity to compete for binding sites in rat forebrain homogenates with [3H]prazosin, a ligand which selectively binds to adrenergic receptors of the alpha 1 subtype. (alpha R)-erythro-alpha-Methylepinephrine is devoid of apparent biological activity, but the activity of the alpha S isomer is substantial. The latter is less active than the endogeneous catecholamines, (R)-norepinephrine and (R)-epinephrine, but the stereospecific competition for [3H]prazosin binding sites by the catecholamine isomers with the beta R configuration is additional evidence that (alpha S)-erythro-alpha-methylepinephrine may be a biologically active metabolite of L-alpha-methyl-3,4-dihydroxyphenylalanine.

Animals↗
PubMed1981

Malignant hyperthermia in myelomeningocele: a previously unreported association.

Malignant Hyperthermia is a life-threatening crisis frequently triggered by the use of various anesthetic agents. The syndrome of susceptibility has been observed in association with a number of orthopedic conditions seen in children but not with myelomeningocele. We have recently seen three children in our Spina Bifida Clinic with the syndrome and we wish to add myelomeningocele to the list of conditions that have a possible association to malignant hyperthermia.

Child, Preschool↗
PubMed1980

Photoaffinity labeling of rat androgen binding protein.

The photoinactivation and photoaffinity labeling of androgen binding sites present in cytosol prepared from intact sexually mature rat epididymides have been demonstrated by using unlabeled and [3H]-labeled 17 beta-hydroxy-4,6-androstadien-3-one. Both photoinactivation and photolabeling are dependent upon exposure to light. These processes are inhibited when photolysis is conducted in the presence of the photoinert compound 17 beta-hydroxy-5 alpha-androstan-3-one, suggesting that steroid-specific sites are involved in the reactions. The labeled steroid-specific product of photolysis is macromolecular with a molecular weight of 47,000 as determined by electrophoresis on polyacrylamide gels containing NaDodSO4, and proteinaceous because digestion of the cytosol with pronase before photolysis eliminates steroid-specific binding. After photolysis, the protein-steroid complex has the ability to withstand dissociation during electrophoresis under denaturing conditions, and unlabeled 17 beta-hydroxy-5 alpha-androstan-3-one fails to displace the label from the complex. Thus, the binding of [3H]17 beta-hydroxy-4,6-androstadien-3-one to the cytosolic protein is covalent. This steroid-specific product is identified as an androgen binding protein of testicular origin by its comigration with native androgen binding protein on nondenaturing polyacrylamide gels and by its molecular weight which is within the range reported for androgen binding protein subunits.

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