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C A Hanson

Publications and source records attributed to C A Hanson.

116 records · Page 7Linked to original sources
PubMedDate not supplied

Bilateral laparoscopic ovariectomy in standing mares: 22 cases.

OBJECTIVE: To describe a technique for laparoscopic bilateral ovariectomy in standing mares and report the outcome of 22 clinical cases. STUDY DESIGN: Prospective study. ANIMALS OR SAMPLE POPULATION: A total of 22 mares between 4 and 23 years of age, weighing between 360 and 600 kg. METHODS: Mares with normal ovaries, as determined by palpation per rectum, were restrained in standing stocks and sedated with detomidine (0.01 to 0.02 mg/kg intravenously [i.v.]) and butorphanol (0.01 to 0.02 mg/kg i.v.). The laparoscope and instrument insertion sites were infiltrated with 2% lidocaine before incision. One laparoscope portal and two instrument portals were located in each paralumbar fossa. Ovariectomy was accomplished by intracorporeal dissection and ligation of the ovarian pedicles. The two instrument portals in each flank were ultimately connected resulting in a 4 to 5 cm laparotomy to facilitate ovarian removal. RESULTS: No major operative or postoperative complications occurred. Minor complications included incomplete hemostasis of an ovarian pedicle with a single ligature (three mares), transient inappetence, pyrexia and incisional infection. Owner satisfaction and cosmetic results were considered excellent. CONCLUSIONS: Standing laparoscopic ovariectomy appears to eliminate many of the potential complications associated with traditional surgical methods for ovariectomy and avoids the risk of general anesthesia. CLINICAL RELEVANCE: This technique requires minimal laparoscopic instrumentation and will provide surgeons with an alternative approach for bilateral ovariectomy in mares.

Animals↗
PubMedDate not supplied

Cytoimmunologic monitoring and heart transplantation.

Cytoimmunologic monitoring (CIM) combines morphological quantitation of activated lymphocytes and immunoblasts with immunophenotyping of peripheral blood in an effort to identify patients at risk for heart transplant rejection. In this study, CIM and endomyocardial biopsy (EMB) results were correlated in 55 paired blood and tissue specimens obtained from 18 heart transplant patients after operation. We found that 83% of patients with rejection and 81% of patients without rejection had increased levels of morphological activated lymphocytes. Multiple other criteria for determining levels of activation were used without improving the sensitivity or specificity. The use of CD4:CD8 ratios to discriminate between activation caused by rejection and those from viral infections and other inflammatory conditions yielded a sensitivity of 43% and specificity of 56%. The presence of activation markers such as I2, TAC, and T9 did not correlate with either CIM or EMB findings. CD4:CD8 alone was not predictive of cardiac rejection as increased, normal, and decreased CD4:CD8 ratios were seen in patients with and without rejection. Other measurements such as percentage of activated lymphocytes, total lymphocyte count, and whole blood cell count did not correlate with EMB or immunophenotypic findings. These results indicate that neither CIM, as currently conducted, nor immunophenotyping alone is sensitive or specific enough to substitute for EMB in screening for tissue rejection.

Antibodies, Monoclonal↗