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Biomedical subjects

B Wilson

Publications and source records attributed to B Wilson.

At least 199 records · Page 11Linked to original sources
PubMed1985

The distribution of serum albumin in the rat testis, studied by electron microscope immunocytochemistry on ultrathin frozen sections.

The distribution of serum albumin is of interest in the rat testis because this protein is the principal carrier for testosterone in the plasma and interstitial fluid of this species. We have localized extravascular serum albumin in the rat testis at the electron microscope level, using gold particle immunocytochemistry on ultrathin frozen sections of tissue fixed lightly by perfusion. The same localization was obtained with three different antisera. Preabsorption and normal rabbit serum controls were negative, and Western blots of testis extracts showed major activity only at the molecular weight of albumin. Serum albumin occurred in substantial concentration throughout extracellular space in the interstitial tissue, as well as in the space between the boundary layer and the base of the seminiferous epithelium. Immunoreactivity extended between Sertoli cells, as well as around spermatogonia and early primary spermatocytes (to stage 11), but did not traverse the Sertoli-Sertoli junctions that comprise the blood-testis barrier. Macrophages in the interstitial tissue showed some endocytic activity. If perfusion fixation was carried out in a manner that flushed most of the albumin from the interstitial space, then a layer of albumin remained on the surface of Leydig cells and many macrophages but was minimal or absent on the surface of other cell types that are normally in contact with albumin, such as Sertoli cells, spermatogonia, myoid cells, lymphatic endothelium, fibroblasts, or cells of blood vessels.

Animals↗
PubMed1985

Adapting "Portage" for neurological patients.

Portage is a home-based teaching technique for mentally handicapped children. It would also appear to be useful for some neurologically impaired people. This paper describes how Portage has been adapted for brain-damaged adults. Three examples are given of Portage type programmes. The first describes the use of checklists for assessment of a blind aphasic woman who had suffered anoxia. The second describes part of the treatment of a woman who developed encephalitis which left her with quadriplegia. The final example outlines the treatment of a patient who had a subarachnoid haemorrhage which resulted in a fear of walking alone.

Activities of Daily Living↗
PubMed1985

Doing sales 'right'.

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Laboratories, Dental↗
PubMed1984

Polymeric complexes and fragments of albumin in normal human plasma.

Nitrocellulose blots of normal human plasma proteins separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis were examined for polymeric complexes and fragments of albumin using an immunoperoxidase-labelled mouse monoclonal anti-human albumin antibody. Under reducing conditions, no polymeric complexes were seen. Under non-reducing conditions, polymeric complexes were detected at the following molecular weights: 210 000, 168 000, 147 000, 132 000, and 110 000. These probably represent both homo- and heteropolymers of albumin. Fresh plasma samples were also analyzed by S-200 chromatography with the same results indicating that detection of polymeric complexes was not an artifact of the sodium dodecyl sulfate-polyacrylamide gel electrophoresis technique. In quantitative terms, polymeric complexes constituted 0.3-2.8% of the total albumin present. Fragments of albumin were also seen in normal human plasma with molecular weights of 45 000, 28 000 and 19 000. These fragments probably represent breakdown products of albumin in normal blood, and they constituted less than 2% of the total albumin present.

Antibodies, Monoclonal↗
PubMed1983

A comparison of deaf, normal, and brain damaged adults on a tachistoscopic task.

Printed words and letters, pictures of signed words, and pictures of finger-spelled letters were presented tachistoscopically to deaf, normal, and brain-damaged adults. The findings suggest that (1) the deaf show no cerebral asymmetry for any of the material but found identification of the stimuli very difficult unless a forced-choice recognition procedure was used or the exposure time was very long; (2) both the normal and the brain-damaged groups show left hemisphere superiority for printed material but no cerebral specialization for signed material; (3) the left-right hemisphere difference is more marked in the brain-damaged group regardless of which hemisphere showed most damage. This may be explained, at least in some cases, by impaired transfer across the corpus callosum.

Adolescent↗
PubMed1983

Effects of glyoxylate on photosynthesis by intact chloroplasts.

Because glyoxylate inhibits CO(2) fixation by intact chloroplasts and purified ribulose bisphosphate carboxylase/oxygenase, glyoxylate might be expected to exert some regulatory effect on photosynthesis. However, ribulose bisphosphate carboxylase activity and activation in intact chloroplasts from Spinacia oleracea L. leaves were not substantially inhibited by 10 millimolar glyoxylate. In the light, the ribulose bisphosphate pool decreased to half when 10 millimolar glyoxylate was present, whereas this pool doubled in the control. When 10 millimolar glyoxylate or formate was present during photosynthesis, the fructose bisphosphate pool in the chloroplasts doubled. Thus, glyoxylate appeared to inhibit the regeneration of ribulose bisphosphate, but not its utilization.The fixation of CO(2) by intact chloroplasts was inhibited by salts of several weak acids, and the inhibition was more severe at pH 6.0 than at pH 8.0. At pH 6.0, glyoxylate inhibited CO(2) fixation by 50% at 50 micromolar, and glycolate caused 50% inhibition at 150 micromolar. This inhibition of CO(2) fixation seems to be a general effect of salts of weak acids.Radioactive glyoxylate was reduced to glycolate by chloroplasts more rapidly in the light than in the dark. Glyoxylate reductase (NADP(+)) from intact chloroplast preparations had an apparent K(m) (glyoxylate) of 140 micromolar and a V(max) of 3 micromoles per minute per milligram chlorophyll.

Journal Article↗