Biomedical subjects

B Wang

Publications and source records attributed to B Wang.

At least 541 records · Page 30Linked to original sources

PubMed1998

[Determination of hyperin in Sanguisorba Officinalis L. by high performance liquid chromatography].

An HPLC method for the determination of hyperin in Sanguisorba officinalis L. was investigated. 0.5 g of dried powdered crude drug was weighed accurately in a 50 mL volumetric flask added with 40 mL methanol, supersonicated for 30 min and then made up to volume with methanol. The sample solution was separated on a Shim-pack ODS column(6.0 mm i.d. x 150 mm, 5 microns) with methanol-0.025 mol/L phosphoric acid (adjusted to pH 3.0 by using triethylamine)(50:50) as the mobile phase and detected at UV-370 nm. Calibration curve of hyperin was obtained for the concentration range of 0.046-0.2 microgram. The corresponding regression equation was Y = 154 x 10(4) X-0.28 x 10(4), r = 0.9998. The recovery was 98.7% and relative standard deviation was 1.9%. The results showed that this method is simple, specific and accurate.

Analgesics↗

PubMed1998

[A clinical analysis of 64 cases of pemphigus].

OBJECTIVE: Reasonable therapeutic dosage regimen for pemphigus are analyzed to find the factors related to prognosis. METHODS: A retrospective study is made on 64 cases of pemphigus who were admitted to our hospital between 1986-1996. RESULTS: Sixty-two of 64 cases with pemphigus were treated with systemic administration of corticosteroids. The average dosage of prednisone for controlling the diseases were 0.958 mg.kg-1.d-1 for mild-type of pemphigus (12 cases) and 1.145 mg.kg-1.d-1 for severe-type (50 cases) respectively. Furthermore, 26 of 62 cases were treated with corticosteroids only, and the average dosage of prednisone for controlling the diseases was 1.072 mg.kg-1.d-1 while in 36 cases treated with corticosteroids combined with immunosuppressors, the average dosage of prednisone was 1.178 mg.kg-1.d-1. The rate of side-effects and complications were 15.4% in patients with corticosteroids alone and 36.1% in patients with combined therapy. Six of eight patients who died were diagnosed pemphigus vulgaris and seven with a course of disease less than 3 years. CONCLUSIONS: We recommend a moderate to high dosage of corticosteroids be the first choice to control pemphigus. Those who were effective with steroids are not recommended to use additional immunosuppressors. Patients with pemphigus vulgaris usually have a poor prognosis while those who could survived more than 4 years usually have a better outcome.

Adolescent↗

PubMed1998

[Study on relationship between ovulation inducing effect of drug-acupuncture and endometrial contents of estradiol receptor and progesterone receptor].

OBJECTIVE: To study the effect of Chinese herbal medicine for replenishing Kidney combined with acupuncture in treating anovulation and infertility, and the relationship between its ovulation inducing effect and endometrial contents of estradiol receptor (ER) and progesterone receptor (PR). METHODS: Twenty-nine cases were treated with replenishing Kidney drugs combined with acupuncture for 1-3 months. Patients' ER and PR were measured by immunohistochemical method. And patients were classified according to PR content into PR positive group and mild PR positive group. RESULTS: Fifteen cases of PR positive group, completed treatment for 45 cycles, among them, 40 cycles showed ovulation, the ovulation rate being 88.89%. Ten in 14 cases, who complicated with infertility, became pregnant, the pregnant rate being 71.43%. While in 11 cases of PR mild positive group, 9 complicated with infertility, completed treatment for 33 cycle, 10 cycles showed ovulation (30.30%), and pregnant rate 22.22% (2/9). The difference between the two groups was significant (P < 0.01). CONCLUSION: The replenishing Kidney drugs combined with acupuncture treatment could result a good effect in treating infertility due to anovulation, especially on those with high endometrial PR content.

Acupuncture Therapy↗

PubMed1998

[Effect of substance P on the short-circuit current of nasal mucosal epithelium].

OBJECTIVE: To observe the changes of epithelial apical membrane short-circuit current (Isc) of antigen-sensitized rat nasal mucosa with the stimulation of Substances P (SP). METHODS: The epithelial apical membrane Isc were measured with Ussing chamber technique, and and we observed the blockage to the effect of SP by neurokinin receptor antagonist Cp96345, histamine H1 receptor antagonist pyrilamine, H2 antagonist ranitidine and neurotoxin respectively. RESULTS: The Isc increased significantly with the stimulation of SP. The effect of SP on Isc could be blocked significantly by pretreatment with four substances. CONCLUSION: SP releasing from nervous endings plays the role on elicit a series of pathological changes like enhanced Isc, epithelial permeabilities, etc.

Animals↗

PubMed1998

A brief history of diagnostic cytology.

Being a discipline specialized for diagnosis of tumors, diagnostic cytology has a history of over 100 years. By exploring the origin, development and its formative process, valuable and special information which are helpful to the general investigation of tumor cases and screening of such patients can be provided.

Cell Biology↗

PubMed1998

[Histamine-1 and -2 receptors in hepatic tissues of cirrhotic patients with portal hypertension].

OBJECTIVE: To study histamine-1 and histamine-2 receptors in hepatic tissues of cirrhotic patients with portal hypertension. METHOD: Light autoradiography was used. RESULT: The density of histamine-2 (H-2) receptors (cirrhotic patients/controls) in hepatocytes was 168.4 +/- 22.6/511.9 +/- 37.6 (P < 0.01), in hepatic vein 238.8 +/- 40.7/313.0 +/- 52.4 (P < 0.01), in hepatic artery 55.6 +/- 7.1/175.4 +/- 26.1 (P < 0.01), and in portal vein 52.0 +/- 5.4/166.4 +/- 17.7 (P < 0.01). The density of histamine-1 receptors in hepatocytes, hepatic vein, hepatic artery and portal vein was not statistically changed between cirrhotic patients and controls (P > 0.05). CONCLUSION: The density of H-2 receptors in hepatic tissues of cirrhotic patients decreased significantly.

Adult↗

PubMed1998

[Application of maxillary bone eversion reduction on benign tumor in nasal cavity and paranasal sinus invading the skull base].

OBJECTIVE: To investigate the operative methods for benign tumors in nasal cavity and paranasal sinus invading the skull base. METHODS: Six cases of benign tumors in nasal cavity and paranasal sinus invading the skull base were reported. Of them, 3 were ossifying fibromas, 1 osteoma, 1 osteochondroma and 1 giant osteoid osteoma. The tumors were removed by combined approaches through unilateral maxillary bone eversion reduction. RESULTS: No complication was found. Follow-up was made 2-3 years after operation. Five cases were uneventful, 1 had a relapse of tumor but still alive. CONCLUSIONS: The method is appropriate for treating benign tumors in nasal cavity and paranasal sinus invading the skull base.

Adult↗

PubMed1998

[DNA content, expression of proliferating cell nuclear antigen and surgical margins in locally advanced laryngeal cancer].

OBJECTIVE: To probe into the relationship between DNA content, PCNA and prognosis. METHODS: Surgical margins in 34 patients with locally advanced laryngeal cancers were observed by means of antibody to PCNA and DNA content measurements. RESULTS: The results demonstrated that at neoplasm margins of 1.0, 0.5, and 0 cm, PCNA indices were 8.62%, 17.76% and 50.32% and DNA indices were 0.98, 1.082 and 1.436 respectively. There is a significant difference between 1.0, 0.5 and 0 cm. CONCLUSION: Moderate and severe atypical hyperplasia of epithelium (AHE) was different from mild AHE and simple hyperplasia of epithelium in the biologic behavior of fission, proliferation and so on and surgical margins should be kept outside the area of moderate AHE.

Adult↗

PubMed1998

[Effect of 8-(N,N'-diethylamino)-n-octyl-3,4,5-trimethoxybenzoate on [Ca2+]i and the release of NO in cultured endothelial cells of the calf middle cerebral artery].

The effect of 8-(N,N'-diethylamino)-n-octyl-3,4,5-trimethoxybenzoate(TMB-8) on [Ca2+]i and the release of NO in cultured endothelial cells of the calf middle cerebral artery was studied by a system of measurement of AR-CM-MIC, using Fura-2/AM as a fluorescent indicator and the oxy-haemoglobin method. In the presence of extracellular Ca2+ 1.3 mmol.L-1, the resting [Ca2+]i and the extinction difference(delta E) of between 401 and 410 nm were not changed by TMB-8 12.5 and 25 mumol.L-1, but were increased by TMB-8 50 and 100 mumol.L-1. The rise of delta E induced by TMB-8 50 and 100 mumol.L-1 was blocked completely by L-NAME. The elevation of [Ca2+]i was related to the release of NO. The result suggested that TMB-8 increased resting [Ca2+]i of endothelial cells of the calf middle cerebral artery and induced NO release.

Animals↗

PubMed1998

[In vitro sensitivity of oral gram-negative bacteria to the bactericidal activity of defensins].

Oral gram-negative bacteria, including Actinobacillus actinomycetemcomitans, Porphyromonas gingivalis, Capnocytophaga sputigena, Provotella intermedius, Provotella melaninogenicus, have been associated with destructive periodontal infection. An ultrasensitive antibacterial assay was applied to determine the sensitivity of a battery of oral gram-negative bacteria to the bactericidal effects of the isolated human neutrophil defensins (HNP) and two kinds of rabbit defensins NP1 and NP2. All species tested were killed by HNP, NP1 and NP2 except that porphyromonas gingivalis was resistant to HNP. However, there was strain-to-strain variation in sensitivity. It was concluded that the oral gram-negative bacteria were sensitive to the cidal mechanism involved in defensinmediated bacterial killing.

Aggregatibacter actinomycetemcomitans↗

PubMed1998

[Mechanisms underlying opioid addiction: new progress].

The mechanisms of opioid addiction has attracted much attention of researchers around the world. In the present review, we have discussed the recent progress in the following topics. (1) Neurotransmitters participating the mechanism of opioid addiction; (2) Roles of different opioid receptors in opioid addiction; (3) Possible involvement of learning and memory in opioid addiction; (4) The intracellular signal transductive pathways after the activation of opioid receptors. Possible method of prevention and treatment of opioid addiction are also discussed.

Animals↗

PubMed1998

[Construction of the prokaryotic expression plasmid pYNenv encoding the gp120 env gene of human immunodeficiency virus type 1 of yunnan strain].

The recombinant envelope protein of human immunodeficiency virus type 1(HIV-1) of Chinese Yunan epidemic strain was expressed in E. coli for confirming the antigenic domains of the protein. The recombinant prokaryotic expressive plasmid pYNenv was constructed by inserting the env DNA fragment which encode the gp120 of HIV-1 Yunnan strain into pBV220. The env DNA fragment was obtained by nested polymerase chain reaction with the HIV-1 infected patients peripheral blood macrophage and monocyte genomes from Yunan HIV-1 epidemic area as template. The recombinant plasmid pYNenv was confirmed by restriction enzyme analysis. The recombinant protein was expressed in E. coli DH10b by pYNenv after vibration culture at 30 degrees C for 20 hours then at 42 degrees C for 5 hours and confirmed by SDS-PAGE electrophoresis. The protein can specifically react with the serum of HIV-1 infected patient from Yunan epidemic area by Western blot assay. The result showed that the recombinant protein can be used as antigen for detection of HIV-1 membrane glycoprotein antibody as well as for the further study of the role of envelope protein in pathology.

Adult↗

PubMed1998

[Detection of proviral gene of human immunodeficiency virus type 1 from dried blood spots].

A method for detection of proviral human immunodeficiency virus type 1 DNA fragment encoded pol gene in dried blood spots on filter paper by nested primers polymerase chain reaction (nPCR) has been developed. The filter paper was pre-saturated and dried by EDTA proteineas K. About 50 microliters whole blood from HIV1 infected individuals was collected and onto the piece of filter paper, and the paper was air dried stored sealed in a plastic bag at 4 degrees C or room temperature for 1 to 64 weeks. The PCR with outer primers was performed in 0.5% ml tube with the piece of filter paper. Following the PCR with the inner primers was finished. The results showed that template DNA could detected in piece of filter papers which stored at 4 degrees C for 40 weeks as well as stored at room temperature for 24 weeks. The results could directly determined by ethidium bromide staining of the PCR products when it compared with the PCR products of standard positive control and DNA molecular weight marker in electrophoresis analysis on agarose gel. The method is rapid, specific, and sensitive and has the ability to detect as little as 10 proviral template DNA. The collected samples could mail from basic laboratory to center laboratory and the method could be applied in confirming and screening of HIV1 infection.

Blood Stains↗

PubMed1998

[Studies on reduction of hexavalent chromium by fungi].

Several fungal strain, isolated from activated sludge, sewage and soil, were able to grow in the medium containing 300-500 mg/L K2Cr2O7, and strain BS-1 was capable of resistance to K2Cr2O7 up to 900 mg/L. After 4-6 d growing of the strain BS-1 and other 3 strains in the liquid medium added 200 mg/L K2Cr2O7, the Cr(VI) content of the medium disappeared completely. These strain were identified as Penicillium sp. BS-1, BS-3, Aspergillus niger BR-4, Aspergillus flavus BX-1 respectively. The results from the detection of the fungal culture by UV-absorbance spectrum and chemical analysis showed that high-toxic Cr(VI) was reduced to lesstoxic Cr(III). Reaction conditions and effectors of Cr(VI) reduction by intact cells of Penicillium sp. BS-1 were studied. The optimum temperature was 30 degrees C, the optimum pH was 7.0. Addition of glucose (0.25%) enhanced Cr(VI) reduction, but high concentrations of Cr(VI) inhibited the reduction of Cr(VI) to Cr(III).

Aspergillus↗

PubMed1998

[Mechanism of specific effects of transient electromagnetic pulses on cell model].

In this paper, a facilitated cell model has been built to analyze the electromagnetic pulses (EMP) induced transmembrane potentials. The analysis shows that the athermal biological effects are caused by micropores on the membrance, not directly by transmembrane potentials. These micropores are enlarged by weak transient EMP and finally lead to membrane openings to make ions cross. The electroporation effects are used to explain the specific response of biological cells to transient EMP.

Cell Membrane↗

PubMed1998

[Karyotype analysis on Iris lactea ssp. chinensis].

In this paper, the chromosome number, karyotype and chromosome volume of Iris lactea ssp. chinensis were reported. The normal diploid was 2n = 32. Karyotype formula was based on Levan's publication in 1964, k (2n) = 32 = 22 m + 8 sm + 2T. According to the method of Kuo SR, the species chromosome based on the relative length was 2n = 32 = 6L + 4 M2 + 18 M1 + 4 S which belong to "2A" type according to the Stebbins' karyotype classification. The AS.K% was 62.5% from Arano's method. The total volume of chromosome groups was 34.95 micron 3.

Base Sequence↗

PubMed1998

[Research on the mechanism of Yin-enriching and Qi-reinforcing, strengthening body and tonification deficiency syndrome action of radix Panacis quinquefolii].

Radix Panacis Quinquefolii could significantly promote 3H-TdR infiltrating of spontaneously and ConA-stimulated splenic lymphocytes of mice; Promote the splenic lymphocytes capacity of mice in interleukin2 (IL-2) production; Significantly enhance erythropoitin (EPO) level of mice in serum; Markedly promote production of colony stimulating factors (CSFs) in murine spleen-conditioned medium (SCM); And promote production of CSFs in lung-conditioned medium (LCM) of mice. The results suggest that yin-enriching and qi-reinforcing, strengthening body and tonification deficiency syndrome action of Radix Panacis Quinquefolii is in association with its lymphocytes activation-stimulating, IL-2 secrection-promoting, EPO secrection, and CSTs production enhancing effectes.

Animals↗

PubMed1998

[Effect on preparing curved artificial root cancals with five handle endodonic instruments I.comparison of cutting ability]

OBJECTIVE: To compare the cutting ability of five handle endodontic instruments. METHODS: The speciments weight loss following serial preparation of artificial root canal were measured with electronic analysis balance. RESULTS: The greatest cutting ability occurred with H file,then with K-flex file,Unifile,K file and K reamer. CONCLUSION: The cutting ability of endodontic instrument is influenced by several factors,such as metallurgical properties,cross-sectional configuration of shaft,flute design and tip design.

Journal Article↗