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Biomedical subjects

B Teisner

Publications and source records attributed to B Teisner.

At least 199 records · Page 11Linked to original sources
PubMed1979

The relation between the concentration of placental specific proteins in retroplacental and peripheral blood.

Concentrations of four placental proteins: human placental lactogen (hPL), placental protein 5 (PP5), pregnancy specific beta 1 glycoprotein (SP1) and human chorionic gonadotrophin (hCG), and a normal serum component, alpha 2 macroglobulin, were measured in the peripheral circulation and in blood obtained from the retroplacental space in 20 women at term delivery. Levels of hPL and PP5 were higher in the retroplacental blood than in the peripheral circulation in all patients. By contrast, levels of SP1 and hCG were consistently lower in retroplacental blood than in the peripheral circulation. Similarly, levels of alpha 2 macroglobulin were lower in the retroplacental blood. It is suggested that this 'reverse' gradient is a technical arterfact. These findings are discussed in relation to synthesis of placental proteins in a site distal to the retroplacental space, and the introduction of a technical artefact in the collection of samples.

Chorionic Gonadotropin↗
PubMed1979

Demonstration of an analogue to human pregnancy zone protein in the African green monkey.

A serum protein in the grivet (Cercopithecus aethiops), immunologically cross-reacting with human pregnancy zone protein (PZP), is found in males as well as in nonpregnant females. The electrophoretic mobility, antigenic cross-reactivity, molecular weight, and carbohydrate structure appear analogous to PZP; however, contrary to the situation in man, exogenous estrogens cause a fall in serum concentration of the protein.

Animals↗
PubMed1979

Characterization of two pregnancy associated serum proteins with pregnancy specific beta 1-glycoprotein (PS-beta-G)-determinants.

The existence of two pregnancy associated, partially identical glycoproteins with PS-beta-G determinants were demonstrated in serum from pregnant women. Crossed and crossed-line immunoelectrophoretic analysis of pregnancy sera revealed two precipitates with alpha- and beta-mobility, respectively. The ratio between the precipitation areas in various samples could be related to three different types of precipitation patterns observed in rocket immunoelectrophoresis. This variation in precipitation pattern could so far not be related to the progression of any obstetric parameters. The diphasic shape of the precipitate in crossed immunoelectrophoresis was not changed by incubation with normal human male serum or antibody to normal human serum. Incorporation of 4% PEG in the gels enhanced the precipitate in the alpha-region. The cross-reactive glycoproteins were detected in all pregnant women examined. These results point to the need for specific antibody preparations permitting separate quantitation of the two antigen populations with PS-beta-G determinants.

Chemical Precipitation↗
PubMed1979

Effect of pregnancy zone protein on leucocyte migration inhibition, lymphoycte transformation and rosette formation by lymphocytes.

Pregnancy zone protein from pregnancy serum and control preparations from male serum were tested in lymphocyte transformation tests, in a system for rosette formation by T and B lymphocytes and in leucocyte migration inhibition tests using PPD-induced inhibition. PZ-protein preparations caused a dose dependent inhibition of lymphocyte transformation after stimulation with PHA, PPD and MLC. Inhibition was most pronounced in the PHA-system. In the rosette tests there was a tendency for T lymphocytes to bind fewer SRBC after incubation with PZ-protein while no effect on B rosettes was observed. In contrast, PPD-induced inhibition of leucocyte migration was abrogated effectively by PZ-protein, but not by corresponding fractions from male serum.

B-Lymphocytes↗
PubMed1978

Two pregnancy-associated serum proteins with pregnancy-specific beta1-glycoprotein determinants.

A protein showing strong antigenic cross-reactivity with the "pregnancy-specific" beta1-glycoprotein (PSbetaG) was demonstrated in serum of pregnant women during the second and third trimesters, but not in sera from normal men and nonpregnant women. The described protein had alpha2-electrophoretic mobility and its molecular weight was estimated to be around 200,000 as compared to 80,000 for PSbetaG. Crossed immunoelectrophoretic analysis, using antiserum to PSbetaG, indicated the presence of additional antigenic determinants in PSbetaG not demonstrable in the cross-reacting alpha2-protein. The PSbetaG/alpha2-protein ratio was rather constant in consecutive serum samples from one woman during pregnancy, but showed a marked variation when sera from different women were compared. Results of rocket immunoelectrophoresis gave a rough estimate of the ratio between these two proteins in a sample of pregnancy serum. The reported findings point to the need for development of specific methods for quantitation of the two proteins and the necessity to re-evaluate earlier quantitative PSbetaG data.

Blood Protein Electrophoresis↗
PubMed1978

Affinity chromatographic purification of the pregnancy zone protein.

An immunospecific affinity chromatographic method for purification of human pregnancy zone protein (PZP) directly from serum is described. Highly purified goat-anti-human PZP-immunoglobulin was applied as a ligand. Recovery of PZP varied from 56--75%. The impurities constituted maximally 5--10% of the total protein in the eluate. The purification factor was approximately 100.

Blood Proteins↗
PubMed1978

An immunoprecipitation-dissociation technique for large scale antibody purification and an antigen consumption electroimmunoassay for antibody quantitation. A model study with antibodies to pregnancy zone protein.

A simple immunoprecipitation--dissociation technique for large scale purification of antibodies is described, which comprises selective denaturation of the antigen and recovery of the antibody fraction by exclusion chromatography at low pH. Its use is illustrated by the purification of antibodies to pregnancy zone protein. A purification factor of about 60 was achieved. An antigen consumption electroimmunoassay was also developed which permits quantitative determination of the antigen binding activity of antibodies with a given specificity. The methods have general application.

Antibodies↗
PubMed1978

An automatic multi-programmed affinity chromatographic system.

An automatic immunospecific affinity-chromatographic system for continuous operation is described. The system comprises time-controlled sample application, washing and elution steps and automatic dialysis of eluted fractions. The applicability of the system is illustrated by the purification of pregnancy zone protein on immunosorbent gel.

Blood Proteins↗
PubMed1978

Immunosuppressive effect of human pregnancy zone protein on H-2 incompatible mouse heart allografts.

Human pregnancy zone protein (PZP) given intraveneously, significantly (p less than 0.001) prolonged the survival of heterotopic A/J heart transplants in A.CA recipients, compared to untreated control recipients, recipients treated with phosphate buffered saline and recipients treated with a control preparation from male serum. The results support the hypothesis that PZP protein exerts an unspecific immunosuppressive effect in vivo.

Animals↗
PubMed1978

Studies of pregnancy-associated murine serum proteins.

A pregnancy-associated murine protein (PAMP) with alpha1 electrophoretic mobility and an estimated molecular weight of 140,000 was present in serum from pregnant and sexually mature non-pregnant females, but could not be detected in the serum of males. During pregnancy the serum concentration of alpha1-PAMP rose to a maximum in mid-pregnancy followed by a decline to preconception levels before parturition; the protein was also present in fetal and neonatal serum and in amniotic fluid at low concentrations.

Animals↗
PubMed1977

Trophoblastic tumour, oral contraceptive therapy and pregnancy associated alpha2-glycoprotein (PAAG), case report.

Pregnancy associated alpha2-glycoprotein (PAAG) and human chorionic gonadotrophin (HCG) levels were studied in a patient with an invasive mole who was being treated with cytotoxic drugs and oral contraceptives. Both the PAAG and HCG levels fell during cytotoxic drug treatment while oral contraceptives caused a rise in PAAG concentrations. A relation between oestrogen-induced PAAG and prolonged survival of trophoblastic tumour is proposed.

Adult↗
PubMed1975

The influence of hyperthermia and poly I:C on Semliki Forest virus infection in mice.

In mice infected intraperitoneally with 10(3) intracerebral suckling mice LD50 of Semliki Forest virus an additive reduction in mortality was found when the protective effect of hyperthermia and synthetic polyriboinosinic and polycytidylic aicds (Poly I:C) was combined. The effects of the two treatments seem to be independent of each other as Poly I:C was found to have no influence on the body temperature of mice, nor did hyperthermia increase the amount of interferon induced by Poly I:C.

Animals↗
PubMedDate not supplied

Aminopropeptide of human procollagen type I: a marker for the identification of blood from children in the mosquito blood meal.

A capture enzyme-linked immunosorbent assay (ELISA) to distinguish between blood from children and adults in the mosquito blood meal was examined using the alpha 1 chain of the aminopropeptide of human procollagen type I (PINP) as antigenic marker. Rabbit anti-human PINP (alpha 1) antibody was used as catching antibody, and either normal serum from 288 African and 58 Caucasian children and adults, or blood meals from 93 fed Aedes aegypti, were examined. PINP in excess of 40 optical density units (ODU) was detected in all children aged 0-13 years, whereas adults aged 21-77 years had PINP levels less than 25 ODU. In the transitional age group (14-20 years), the PINP levels ranged from 1 to 166 ODU. The PINP levels in 95% of the mosquito blood meals obtained from children exceeded the control levels, using 13 ODU as a cut-off value, whereas none of the blood meals from adults exceeded 13 ODU. The PINP levels in the mosquito blood meals were constant 1 and 8 h after ingestion, but they had decreased significantly after 16-19 h. Our data suggest that the test can be used to identify host preferences in studies of mosquitoes collected within 16 h after the blood meal. A field evaluation is necessary to determine the potential of the antigenic marker PINP as a tool in the identification of mosquito host preference.

Adolescent↗
PubMedDate not supplied

Simultaneous autoradiography and line immunoelectrophoresis (ARLIE): a novel combination to identify de novo protein synthesis by pregnancy tissues.

A novel combination of two conventional techniques (autoradiography, AR and line immunoelectrophoresis, LIE; ARLIE) for identification of specific proteins synthesized de novo by explants is described. The incorporation rate of [35S]-methionine was linear in proteins derived from cytosol fractions and supernatants of first trimester human trophoblast and gestational endometrium for up to 18 h. SDS-PAGE analysis of these fractions provided further evidence of the protein synthesis and secretion by the tissue explants. The ARLIE system was evaluated by investigating the synthesis and secretion of five test proteins (PP12, PP14, hPL, FA-1 and FA-2) by trophoblast and gestational endometrium. The synthesis P12 and PP14 could be demonstrated by gestational endometrium only. Similarly the synthesis of hPL could be demonstrated by the trophoblast alone. The synthesis of the fetal proteins (FA-1 and FA-2) could not be demonstrated by either tissue. The control procedure, Protein A assisted immunoprecipitation, yielded similar results for PP14 but not hPL. This novel combination (ARLIE) provides a simple technique with which to study the de novo synthesis of several proteins simultaneously which is independent of the subclass and species of origin of antibodies.

Amniotic Fluid↗
PubMedDate not supplied

The ratio between molecular variants of pregnancy-specific beta 1 glycoprotein (SP1) in the peripheral and retroplacental circulation during later pregnancy.

The ratio between two molecular variants of pregnancy-specific beta 1 glycoprotein (SP1) in the peripheral and retroplacental circulation was analysed in nine women during late pregnancy. The ratio between the two molecular variants, i.e. SP1 beta and SP1 alpha, was found to be identical in the peripheral and retroplacental circulation in all women examined. Comparisons were also made between SP1 beta as measured by radioimmunoassay and a normal serum protein, alpha 2 macroglobulin. A positive concentration gradient from peripheral to retroplacental circulation was found for SP1 beta whereas the gradient was less pronounced for alpha 2 macroglobulin. These findings add weight to the concept that SP1 alpha is a unique molecular species and not a genetic variant or a product of SP1 beta.

Female↗