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Biomedical subjects

B Teisner

Publications and source records attributed to B Teisner.

At least 181 records · Page 10Linked to original sources
PubMed1981

Comparison of pregnancy-associated murine protein-1 and human pregnancy zone protein.

Immunological cross-reaction between human pregnancy zone protein (PZP, SP-3, alpha 2-PAG) and pregnancy-associated murine protein-1 (PAMP-1) was demonstrated by countercurrent line immunoelectrophoresis using antibodies against the proteins raised in hens. The levels of PZP and PAMP-1 were measured during pregnancy and found to be very similar in the first half of pregnancy. Both proteins were found to be heterogeneous and separable according to surface properties. It is suggested that the murine PAMP-1 can be used to study the physiology of human PZP.

Animals↗
PubMed1981

Pregnancy associated plasma protein-A: interaction with heparin in crossed affinity immunoelectrophoresis.

A specific interaction between pregnancy associated plasma protein A- (PAPP A) and heparin has been demonstrated using heparin affinity crossed immunoelectrophoresis applied to late pregnancy serum. The presence of heparin in the first dimensional gel accelerated the anodic migration of six serum proteins with beta electrophoretic mobility and one protein in the alpha mobile region. Two of the six beta mobile proteins were identified as antithrombin III and beta lipoprotein, the alpha mobile protein being PAPP-A. The migration distance of other proteins originating in the placenta (pregnancy specific beta, glycoprotein), fetus (alpha-fetoprotein), leucocytes (pregnancy zone protein), or maternal liver (alpha, antitrypsin alpha 2 macroglobulin) was not altered. The interaction of PAPP-A with heparin was therefore independent of molecular size, charge, and site of origin indicating a specific high affinity interaction between PAPP-A and heparin. There results indicate that PAPP-A may be involved locally with the coagulation system in the maintenance of placental circulation.

Female↗
PubMed1981

Improved diagnosis of neural tube defects: measurement of concanavalin A-reactive variants of AFP in amniotic fluid.

The proportion of alphafetoprotein (AFP) not reacting with Concanavalin A(Con A) was determined by crossed line affinity immunoelectrophoresis (CLAIE) in amniotic fluid obtained from 16 pregnancies with open neural tube defects and other fetal abnormalities, and 25 pregnancies with normal outcome. The percentage of Con A non-reactive AFP was significantly lower in the presence of open neural tube defects (less than 3.9%, n = 13) compared with that in normal pregnancy (range 6.4-25.2%, n = 25). In this retrospective analysis, 2 pregnancies affected by open neural tube defect escaped diagnosis on the basis of an ambiguous total AFP estimation (+2SD less than x less than +5SD) in the amniotic fluid. These observations suggest that the elimination of false diagnose based on total AFP levels may be possible, if supplementary analysis by CLAIE is performed.

Amniotic Fluid↗
PubMed1981

Placenta-associated plasma protein-A (PAPP-A, SP4) in trophoblastic tumours.

The immunoperoxidase technique was used to study the occurrence of placenta-associated plasma protein A (PAPP-A, SP4) in formalin-fixed paraffin-embedded tissue specimens from 15 hydatidiform moles, 6 invasive moles and 7 choriocarcinomas. PAPP-A was found in the syncytiotrophoblast of all hydatidiform moles, in 5 of the 6 invasive moles and in 6 of the 7 choriocarcinomata. No clinical difference was observed between PAPP-A positive and negative cases of invasive disease.

Choriocarcinoma↗
PubMed1981

Serum levels of the "pregnancy-zone protein" during short-term prenatal dexamethasone therapy.

The serum concentrations of the "Pregnancy Zone Protein" (PZP) was followed in 5 patients treated with dexamethasone (12 mg per day for 1 week) in the third trimester of pregnancy. The serum concentration of PZP was found to decrease significantly to an average of 58 per cent of the pretreatment levels. The simultaneous change in serum estriol levels during treatment and other factors of possible importance for the serum concentration of PZP are discussed.

Dexamethasone↗
PubMed1980

Immunochemical and biological studies on two molecular variants of pregnancy specific beta 1 glycoprotein (SP1) SP1 alpha and SP1 beta.

Analytical crossed immunoelectrophoresis was performed to examine the relationship of a high molecular mass alpha 2 mobile glycoprotein (SP1 alpha) which is immunochemically identifiable with pregnancy specific beta 1 glycoprotein (SP1 beta). There was no evidence for any reaction of SP1 alpha with antisera to normal human serum protein, nor for complex formation following incubation with normal serum proteins. The half-life of SP1 alpha was apparently shorter than that of SP1 beta after delivery of the placenta.

Female↗
PubMed1980

A method for preparation of immunosorbents by direct coupling of dissociated immune complexes.

A simple and rapid method of preparing immunosorbents by use of isolated immune complexes is described. Dissociated immune complexes may be directly coupled to activated Sepharose and an immunosorbent gel with complementary molecular populations of immunospecifically purified ligands is obtained. The antigen binding capacity of insolubilized antibodies was about 50% of the antigen bound at equivalence using liquid phase precipitation. The batch technique described may be scaled up and the only limitation is the availability of the ligands. The usefulness of the technique is illustrated in two model systems.

Animals↗
PubMed1980

Pregnancy specific beta-1 glycoprotein (SP1) in patients with trophoblastic disease: molecular heterogeneity and a "SP1 consuming factor".

The reactivity of sera from 19 patients with trophoblastic disease was analyzed in analytic immunoelectrophoresis using rabbit and human pregnancy specific beta-1 glycoprotein (SP1) antisera. Molecular heterogeneity of SP1 was demonstrated in crossed immunoelectrophoresis and a "SP1 consuming factors" was found in serum from a patient with choriocarcinoma. The "SP1 consuming factor" was precipitated by 1.65 M (NH4)2SO4. The results are discussed in relation to quantification of circulating SP1 patients with trophoblastic disease.

Chemical Precipitation↗
PubMed1980

Immunochemical characterization and serum concentrations of pregnancy-associated serum proteins in mice.

The glycoprotein structure of two pregnancy-associated murine proteins, alpha 1-PAMP and alpha 2-PAMP, was analysed by using concanavalin A (Con A) affinity electrophoresis: alpha 1-PAMP was completely precipitated by free Con A, whereas alpha 2-PAMP showed heterogeneity. The isoelectric points of alpha 1-PAMP and alpha 2-PAMP were determined at 4.2 and 4.0 respectively by using crossed immunoelectrofocusing. The alpha 2-PAMP was detectable only in the serum of pregnant mice and fetuses. Maternal serum concentrations increased from Day 8 of pregnancy, remained at high values between Days 14 and 18, and could not be detected by 2 days post partum.

Amniotic Fluid↗
PubMed1980

Comparison of the pregnancy-associated murine protein-2 and human pregnancy-specific beta 1-glycoprotein.

Immunological cross-reaction between human pregnancy-specific beta 1-glycoprotein (SP-1) and pregnancy-associated murine protein-2 (alpha 2-PAMP, PAMP-2) was demonstrated by line immunoelectrophoresis using antibodies against the proteins raised in hens. The pattern of change of PAMP-2 and SP-1 values in serum during pregnancy was very similar. The murine placental growth-rate corresponded to PAMP-2 levels in maternal serum during pregnancy, indicating placental origin. We suggest that the mouse and its PAMP-2 can be used to study human SP-1.

Animals↗
PubMed1979

Pregnancy-specific beta 1-glycoprotein (SP1) determined by means of electroimmunoassay, radial immunodiffusion and nephelometry.

A comparison has been made between rocket-immunoelectrophoresis (RIE), radial immunodiffusion (RID) and automated immunonephelometry (AIP) in the assay of pregnancy-specific beta 1-glycoprotein (SP1) in serum from pregnant women. Using RIE an interaction was demonstrated between the various SP1-reactive molecular populations causing a bias of up to 10%. An interaction corresponding to this phenomenon cannot be demonstrated when using RID and AIP. When correlating the serum-SP1 concentration of samples containing various ratios of SP1-reactive molecules by means of RIE, RID and AIP, it was demonstrated that there was no correlation between the results achieved using one method compared to the results achieved by either of the other methods. The results achieved using one method can therefore exclusively be judged from reference values determined using the same method. The analysis time is essentially shorter with AIP than with RIE and RID.

Female↗
PubMed1979

Molecular heterogeneity of pregnancy specific beta 1 glycoprotein: the effect on measurement by radioimmunoassay and electroimmunoassay.

A comparison of methods for the quantification of circulating pregnancy specific beta 1 glycoprotein in late pregnancy was performed to assess the influence of the presence of a high molecular weight glycoprotein with alpha 2 electrophoretic mobility (SP1 alpha) which is immunochemically identifiable with pregnancy-specific PBETA 1 glycoprotein (SP1 beta). Serum samples from 47 volunteers in the 3rd trimester of pregnancy were subjected to measurements of pregnancy specific beta 1 glycoprotein in rocket immunoelectrophoresis, quantitative crossed-immunoelectrophoresis and radioimmunoassay. Rocket immunoelectrophoresis gives a result which reflects the total SP1 content, i.e. SP1 alpha and SP1 beta while quantitative crossed-immunoelectrophoresis permits differentiation between the two molecules. Radioimmunoassay predominantly measures authenic SP1, i.e. SP1 beta in the presence of physiological amounts of SP1 alpha.

Chromatography, Gel↗
PubMed1979

Specific assay for pregnancy-specific-beta 1-glycoprotein (Sp1-beta). Preparation of antiserum specific for SP1-beta by absorption with a crossreacting high molecular weight serum protein.

Two pregnancy-associated proteins reacting with antibody against pregnancy-specific beta 1-glycoprotein (PS beta G or SP1) were separated by means of ammonium sulphate precipitation, size chromatography and preparative zone electrophoresis. The antibody preparation was made monospecific to the beta-mobile protein by liquid phase cross-absorption of anti-SP1 immunoglobulin preparation with the isolated alpha2-mobile high molecular weight protein. The specificity of the absorbed antibody was tested in line immunoelectrophoresis, rocket immunoelectrophoresis and crossed immunoelectrophoresis.

Absorption↗
PubMed1979

Quantification of the pregnancy-specific beta1-glycoprotein (SP1) by immunoprecipitation techniques: the influence of a cross-reacting high molecular weight alpha2-protein.

Two serum proteins with SP1 determinants were separated by means of size chromatography and preparative zone electrophoresis. One of the proteins had beta1 electrophoretic mobility and an estimated molecular weight of 90,000, and the other had alpha2-mobility and a molecular weight around 400,000. When available antibody preparations to SP1 were used, the high molecular weight protein formed immunoprecipitates only when polyethyleneglycol (PEG) was added to the agarose. It is shown that the high molecular weight protein has a pronounced effect on quantification of SP1 by rocket immunoelectrophoresis, whereas the effect was less pronounced but still considerable when quantification was performed by radial immunodiffusion.

Antigens↗