Search PubMed⌕ Search

Biomedical subjects

B S Jacobson

Publications and source records attributed to B S Jacobson.

68 records · Page 4Linked to original sources
PubMed1975

Carbonic anhydrase of spinach: studies on its location, inhibition, and physiological function.

Carbonic anhydrase activity was determined in spinach (Spinacia oleracea) leaf organelles isolated on sucrose density gradients and was found to be predominantly in the intact chloroplast fraction. The small amount of activity associated with the mitochondrial fractions was probably due to intact chloroplast contamination. No activity could be associated with the broken chloroplast or microbody fractions. Based upon inhibitor studies, carbonic anhydrase was found to be around 2 mm in the chloroplast. Ethoxzolamide, an inhibitor of carbonic anhydrase, reduced CO(2) fixation in intact chloroplasts. The concentration required to inhibit CO(2) fixation 20 to 40% was in excess of that required to inhibit the purified enzyme. The inhibition was partially reversed by CO(2). Ethoxzolamide had no effect on photosynthetic NADP reduction or photophosphorylation measured by methyl viologen reduction. The physiological role of carbonic anhydrase was shown not to be associated with CO(2) diffusion or CO(2) concentration. It is proposed that other functions of carbonic anhydrase could be the protection against denaturation by transient localized changes in pH or the hydration of compounds other than CO(2).

Journal Article↗
PubMed1974

Fat Metabolism in Higher Plants: LXII. Stearl-acyl Carrier Protein Desaturase from Spinach Chloroplasts.

Stearyl-acyl carrier protein desaturase (EC 1.14.99.6), present in the stroma fraction of spinach (Spinacia oleracea) chloroplasts, rapidly desaturated enzymatically prepared stearyl-acyl carrier protein to oleic acid. No other substrates were desaturated. In addition to stearyl-acyl carrier protein, reduced ferredoxin was an essential component of the system. The electron donor systems were either ascorbate, dichlorophenolindophenol, photosystem I and light, or NADPH and ferredoxin-NADP reductase. The desaturase was more active in extracts prepared from chloroplasts obtained from immature spinach leaves than from mature leaves. Stearyl-acyl carrier protein desaturase also occurs in soluble extracts of avocado (Persea americana Mill.) mesocarp and of developing safflower (Carthamus tinctorius) seeds.

Journal Article↗
PubMed1973

Fat Metabolism in Higher Plants: LVII. A Comparison of Fatty Acid-Synthesizing Enzymes in Chloroplasts Isolated from Mature and Immature Leaves of Spinach.

Chloroplasts isolated from immature leaves of spinach (Spinacia oleracea) differ in enzyme levels from those isolated from mature leaves. On a chlorophyll basis, immature chloroplast preparations had 5- to 6-fold higher capacity to synthesize fatty acids from 2-(14)C-acetate compared to plastids isolated from mature leaves. This difference was correlated with higher activities for the enzymes, acetyl coenzyme A synthetase, malonyl coenzyme A synthetase, acetyl coenzyme A carboxylase, and oleyl coenzyme A transferase in plastid pressates obtained from immature leaves. Disrupted chloroplast preparations from both mature and immature leaves retained the ability to incorporate 2-(14)C-acetate into fatty acids in a pattern similar to that by isolated chloroplasts. 2-(14)C-Acetate, 2-(14)C-acetyl coenzyme A, 2-(14)C-malonate, and 1,3-(14)C malonyl coenzyme A were readily incorporated into a number of fatty acids. Moreover, the synthesis of oleate by chloroplast pressates from these substrates was strongly inhibited by KCN, flavin adenine mononucleotides and dinucleotides, and anaerobic conditions, while linolenic acid synthesis was unaffected by these compounds.

Journal Article↗
PubMed1970

The prevalence of carbon-13 in respiratory carbon dioxide as an indicator of the types of endogenous substrate. The change from lipid to carbohydrate during the respiratory rise in potato slices.

Isotope discrimination is a common feature of biosynthesis in nature, with the result that different classes of carbon compounds frequently display different (13)C/(12)C ratios. The (13)C/(12)C ratio of lipid in potato tuber tissue is considerably lower than that for starch or protein. We have collected respiratory CO(2) from potato discs in successive periods through 24 hr from the time of cutting-an interval in which the respiration rate rises 3-5-fold. The (13)C/(12)C ratio of the evolved CO(2) was determined for each period, and compared with the (13)C/(12)C ratios of the major tissue metabolites. In the first hours the carbon isotope ratio of the CO(2) matches that of lipid. With time, the ratio approaches that typical of starch or protein. An estimation has been made of the contribution of lipid and carbohydrate to the total respiration at each juncture. In connection with additional observations, it was deduced that the basal, or initial, respiration represents lipid metabolism-possibly the alpha-oxidation of long chain fatty acids-while the developed repiration represents conventional tricarboxylic acid cycle oxidation of the products of carbohydrate glycolysis. The true isotopic composition of the respiratory CO(2) may be obscured by fractionation attending the refixation of CO(2) during respiration, and by CO(2) arising from dissolved CO(2) and bicarbonate preexisting in the tuber. Means are described for coping with both pitfalls.

Aging↗
PubMed1967

Distribution of an Indoleacetic Acid-oxidase-inhibitor in the Storage Root of Daucus carota.

Indoleacetic acid (IAA)-oxidase from both secondary phloem and xylem was dependent on 2,4-dichlorophenol for activity, and was enhanced by addition of Mn(2+). The pH optimum was 6.0 from both tissues. IAA-oxidase and its inhibitors were distributed differently in the secondary phloem and secondary xylem of carrot root. In the phloem a high IAA-oxidase activity was distributed uniformly along the radius but in the xylem a somewhat lower concentration decreased from the cambium. IAA-oxidase inhibitor in the phloem increased exponentially from a very low concentration near the cambium, whereas in the xylem an appreciable concentration was present near the cambium, decreasing linearly with distance from the cambium. Longitudinal gradients in the xylem parallel studies by other workers with the greatest IAA-destroying capacity present in older tissues. In the xylem inhibitor decreased and IAA-oxidase increased from the root apex. In the phloem IAA-oxidase was uniform, whereas the inhibitor increased in older tissue.The IAA-oxidase inhibitors in phloem and xylem may be different. In the xylem the IAA-oxidase inhibitor may be a lignin precursor present in young cells which disappears as lignification proceeds. In the phloem IAA-oxidase reacting with endogenous IAA appears to form a physiologically active product.

Journal Article↗
PubMedDate not supplied

The role of air pollution and other factors in local variations in general mortality and cancer mortality.

Age-adjusted indices of female cancer mortality and total male mortality in 31 California countries were correlated with air pollution data and with geographic and socioeconomic data from the 1970 United States Census and elsewhere. Air quality was the worst in those countries where socioeconomic status was highest. Cancer mortality showed positive correlations with air pollution levels. Overall male mortality was lower in the affluent but polluted coastal counties. However, when partial correlation coefficients were calculated, controlling for the confounding variables, both overall male mortality and female cancer mortality showed positive correlations with air pollution indices. Stepwise regression analysis produced similar results.

Actuarial Analysis↗