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Biomedical subjects

B Runnebaum

Publications and source records attributed to B Runnebaum.

At least 235 records · Page 13Linked to original sources
PubMed1978

Maternal peripheral testosterone levels during the first half of pregnancy.

Peripheral serum testosterone levels were determined in 180 women during weeks 7 to 20 of pregnancy with a specific radioimmunoassay. After a normal pregnancy and delivery 90 serum samples were randomly selected from mothers of boys and 90 serum samples from mothers of girls. The testosterone concentrations were correlated with the sex of the fetuses. The mean testosterone level +/- S.D. in pregnant women with female fetuses was 597 +/- 167 pg. per milliliter. In pregnant women with male fetuses the testosterone concentrations were on the average significantly higher (p less than 0.01), with a mean value of 828 +/- 298 pg. per milliliter. The course of the testosterone concentrations in women with male fetuses showed an increase beginning in week 7, reaching a maximum during weeks 9 to 11, followed by a decrease until weeks 15 to 20. During weeks 9 to 11 of pregnancy fetal sex determination was possible in 28 per cent of the males and in 5 per cent of the females, with a probability of 95.5 per cent.

Female↗
PubMed1978

[The value of determinations of human chorionic gonatrophin, human placental lactogen, progesterone and oestriol in women with threatened abortion (author's transl)].

During the antenatal care of 604 women 1629 blood samples for hormonal tests were collected. The values in 417 women with normal pregnancies were used to establish a normal curve with 99% tolerance for the serum concentrations of human chorionic gonatrophin (HCG), human placental lactogen (HPL), progesterone and oestriol. Of the 604 women 187 had bleeding during the first half of the pregnancy. Of these 187 women 57 carried the pregnancy to term and 130 had a miscarriage. Women with the symptoms of threatened abortion who carried the pregnancy to term had normal serum hormone values. The HCG values in this group were higher than normal at the time of bleeding. In 90% of the women with threatened abortion who miscarried prior to 16 weeks gestation (N = 87) and in all patients with a missed abortion (N = 16) 1 or more serum hormone concentrations were below normal. Women with early pregnancy bleeding and late abortion between 17 and 28 weeks (N = 27) showed 1 or more low hormone concentration in only 25% of the cases. Our findings show that the quantitative determination of HCG and progesterone give the most valuable tests for the evaluation of the endocrine system in the first trimester of pregnancy. In the second trimester HCG determinations are of value only in trophoblast disease. To a lesser degree determination of HPL and oestriol are of prognostic value in the second trimester of the pregnancy.

Abortion, Threatened↗
PubMed1977

New progesterone metabolites in human myometrium.

Homogenates from human myometrium with an added NADPH regenerating system and ATP were incubated with 4-(14)C progesterone. Six metabolites were identified: 5alpha-pregnane-3, 20-dione, 5beta-pregnane-3, 20-dione, 3alpha-hydroxy-4-pregnen-20-one, 3beta hydroxy-4-pregnen-20-one. 20alpha-hydroxy-4-pregnen-3-one and 20beta-hydroxy-4-pregnen-3-one. The identification of these metabolites was based on their behaviour in paper and thin layer chromatography and especially in radiogaschromatography either as free compounds or after formation of derivatives. The identification of the two allylic metabolites was supplemented by specific microchemical and enzymatic reactions. The formation of 5beta-pregnane-3, 20-dione, 20beta-hydroxy-4-pregnen-3-one and of the two allylic alcohols 3alpha-hydroxy-4-pregnen-20-one and 3beta-hydroxy-4-pregnen-20-one in human myometrium homogenates was demonstrated for the first time.

Adult↗
PubMed1976

Effect of alpha-MSH on plasma levels of LH, FSH, progesterone and cortisol during the corpus luteum phase of the menstrual cycle.

In healthy women (21-28 years) the influence of synthetic alpha-MSH upon the peripheral plasms levels of LH, FSH, progesterone and cortisol was determined during the corpus luteum phase of the menstrual cycle. As controls 3 women were given 6 intravenous infusions of 250 ml NaCl; 4 women received a total of 18 intravenous infusions of 5-20 mg alpha-MSH from 9.00 to 11.00 a.m. on the 5th and 7th hyperthermic day of the menstrual cycle. The blood levels of the hormones were usually followed for 24 h, and in two cases for 48 h. During and after the control as well as the experimental infusions with 5-20 mg alpha-MSH, no significant changes in the plasma concentrations of LH, FSH and progesterone were found. The cortisol concentrations, however, showed on the average a 2-fold increase over the initial values during the infusion of 5 mg and 10 mg alpha-MSH. During the control infusions they were not enhanced. One experiment was conducted with 20 mg alpha-MSH. The increase in the plasma cortisol levels following alpha-MSH administration generally seemed to be dose dependent, but statistically no significant differences regarding the increase in cortisol level could be detected between the 5 mg and 10 mg doses.

Adult↗
PubMed1975

Progesterone 20 alpha-dihydroprogesterone and 20 beta-dihydroprogesterone levels in different compartments from the human foeto-placental unit.

The endogenous levels of progesterone, 20 alpha-dihydroprogesterone and 20 beta-dihydroprogesterone in different foetal tissues, in placental tissue and in foetal blood during pregnancy (week 9-25) were determined by gas-liquid chromatography or by radioimmunoassay. The identification of these steroids in foetal tissues was based on the behaviour in paper and thin-layer chromatography, the formation of different derivatives, and the retention times in gas-liquid chromatography. For additional identification of progesterone and 20 alpha-dihydroprogesterone a mass spectrum was obtained. The average concentrations of progesterone (P), 20 alpha-dihydroprogesterone (20 alpha-DHP) and 20 beta-dihydroprogesterone (20 beta-DHP) in foetal tissues were as follows (ng/g wet weight tissue): brain, P 119.0 +/- 45.5, 20 alpha-DHP 86.9 +/- 44.7 and 20 beta-DHP 9.7; lungs, P 257.7 +/- 195.7, 20 alpha-DHP 60.3 +/- 26.4 and 20 beta-DHP 7.8; liver, P 103.7 +/- 84.6, 20 alpha-DHP 95.2 +/- 89.8 and 20 beta-DHP 17.8; adrenals, P 295.7 +/- 90.0 20 alpha-DHP 254.5 +/- 194.6; kidneys, P 214.2 +/- 155.3 and 20 alpha-DHP 76.6 +/- 43.0; intestine, P 273.2 +/- 166.7, 20 alpha-DHP 77.9 +/- 51.2 and 20 beta-DHP 8.7; residual tissues, P 246.3 +/- 178.3 and 20 alpha-DHP 54.8 +/- 44.5. The average concentrations in placental tissue and in foetal plasma (week 12-18) were as follows (ng/g wet weight tissue or ng/ml plasma): placenta, P 2619. +/- 2153.6 and 20 alpha-DHP 461.0 +/- 155.9; foetal plasma, P 378.7 +/- 231.8 and 20 alpha-DHP 283.0 +/- 108.9. These results indicate that some foetal organs contain a high 20 alpha-reductase activity at least during the first half of pregnancy.

20-alpha-Dihydroprogesterone↗
PubMed1975

Progesterone, 20 alpha-dihydroprogesterone and 20 beta-dihydroprogesterone in mother and child at birth.

In 14 healthy pregnant women at term progesterone (P) and 20 alpha-dihydroprogesterone (20 alpha-DHP) were determined by radioimmunoassay both in the maternal and foetal compartments. The average concentrations were as follows (ng/ml blood or ng/g wet weight tissue): peripheral maternal blood P 56 +/- 26, 20 alpha-DHP 16 +/- 8; placental tissue P 2514 +/- 1516, 20 alpha-DHP 429 +/- 412; placental blood P 365 +/- 160, 20 alpha-DHP 35 +/- 18; blood of the umbilical vein P 388 +/- 121, 20 alpha-DHP 33 +/- 15; blood of the umbilical arteries P 162 +/- 62, 20 alpha-DHP 28 +/- 10. In 5 healthy pregnant women at term progesterone (P), 20 alpha-dihydroprogesterone (20 alpha-DHP) and 20 beta-dihydroprogesterone (20 beta-DHP) were also determined by gas-liquid chromatography both in the maternal and foetal compartments. The average concentrations were as follows (ng/ml plasma or ng/g wet weight tissue): peripheral maternal plasma P 129 +/- 49, 20 alpha-DHP 15 +/- 15, 20 beta-DHP 1.7 +/- 0.9; placental tissue P 5060 +/- 1435, 20 alpha-DHP 230 +/- 158, 20 beta-DHP 38 +/- 30; placental plasma P 723 +/- 245, 20 alpha-DHP 24 +/- 13, 20 beta-DHP 1.0 +/- 0.1; plasma of the umbilical vein P 740 +/- 227, 20 alpha-DHP 17 +/- 3, 20 beta-DHP 1.2 +/- 0.3; plasma of the umbilical arteries P 324 +/- 94, 20 alpha-DHP 17 +/- 5, 20 beta-DHP 2.6 +/- 2.1. These results show that, contrary to the progesterone concentrations, no significant difference exists in the concentrations of 20 alpha-DHP and 20 beta-DHP between the blood from the umbilical vein and arteries. Furthermore, no significant difference could be found in the concentrations of P and 20 alpha-DHP between the sexes in either the blood from the umbilical vein or from the umbilical arteries.

20-alpha-Dihydroprogesterone↗