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Biomedical subjects

B Rassow

Publications and source records attributed to B Rassow.

At least 19 recordsLinked to original sources

[Possible damage to the eye caused by light from ophthalmologic equipment].

Motivated by numerous findings of the damaging effect of intense light levels on the fundus we measured the spectral radiation of several ophthalmic illuminations and used it to calculate from it the spectral retinal irradiance. To evaluate the thermal hazard, we calculated the resulting temperature rise at the fundus. A measure for biological damage is the percentage of denaturated molecules, which is related to the temperature rise and its duration (essentially identical to the duration of illumination) by the so-called Arrhenius-integral. We used this measure to compare any illumination with experimentally evaluated threshold values for thermal damage. To evaluate the photochemical hazard we compared the spectral retinal irradiance with threshold values for photochemical induced lesions. Furthermore the illuminations were correlated with safety-guidelines likewise distinguishing between thermal and photochemical hazard. Under adverse conditions operation microscopes can present a thermal hazard to the patient's eye. Other ophthalmic illuminations are of less concern in this respect. A similar estimation applies to hazard from photochemical effects. The photochemical hazard can be diminished essentially by reducing the short-wavelength bluelight. Qualitatively, maximal permissible exposures according to safety guidelines are consistent with our thermal calculations as well as with photochemical hazard evaluation by comparing spectral retinal light levels with threshold irradiance for photochemical induced lesions. However, since quantitatively safety guidelines estimate the thermal hazard very conservatively, many common ophthalmic illuminations exceed the exposure limits, in some cases even drastically.

Hot Temperature

[Comparison of the Lithander (Kolt test) pediatric visual acuity test with the standardized Landolt ring test].

The visual acuity values obtained with the four test figures of Lithander's "Kolt-test" have been compared with Landolt ring acuity under standardized conditions which meet the requirements of DIN 58,220 and ISO/DIS 8596/7. For a given test figure size, the Kolt-test optotypes turned out to be somewhat easier legible than the Landolt ring. On average, visual acuity measured with the Kolt-test exceeded Landolt ring acuity by 0.08 log (angle of resolution) units. Thus, according to DIN and ISO criteria, the legibility of the Kolt-test is not equivalent to the standard Landolt ring. Equal acuity values could be obtained by increasing the observation distance proposed by the manufacturer of the Kolt-test by 19%. The legibility of the four optotypes differs on adult subjects: "circle" is more difficult, "cross" is easier legible than "square" and "triangle". Confusion of Kolt-test figures is basically restricted to two pairs of two optotypes ("cross" - "triangle", "square" - "circle").

Adult

Absolute ocular fundus dimensions measured by multiple-beam interference fringes.

Multiple-beam interference fringes have been projected onto the retina to serve as a size reference for fundus photography. The absolute fringe spacing is determined by measurable parameters. The interference fringes are extremely narrow so that fringe maxima and fundus structures can be perceived equally well, and a high precision of measurement is achieved. The method has been applied to a model eye of varying ametropia (within +/- 7 diopters). The reproducibility of measurement is within 0.5%. In vivo measurements on human eyes demonstrate the clinical applicability.

Fundus Oculi

[Determination of contrast sensitivity].

Measurement of contrast sensitivity function (CSF) for obtaining diagnostic information on the visual system has been strongly advocated in the US literature during the last few years. The basic concepts of this type of measurement, and in particular the definition of contrast for periodic objects, are explained here. The advantages and disadvantages of different methods of measuring CSF and the correlation between CSF and visual acuity are reviewed. The article concludes with a discussion of the clinical relevance of these measurements.

Color Perception Tests

Electrophysiological assessment of the human depth-perception threshold.

The cortical response to stereoscopic stimuli was measured as a function of disparity using visual evoked potentials (VEP). The stereoscopic stimulus consisted of a dynamic random-dot pattern that portrayed a three-dimensional horizontal grating. Disparity of the grating was variable between 0 and 18 arc min, step size being 4.22 arc s. Evoked responses were recorded using a "random-sequence sweep technique." The VEP amplitude increased approximately linearly with the logarithm of disparity and allowed an estimation of the stereo threshold by linear extrapolation. The evoked potential estimates of the stereo threshold of 16 subjects were compared to psychophysically obtained thresholds. Several threshold-estimation techniques are discussed. Evoked potential and psychophysical threshold estimates had the lowest discrepancy when the VEP amplitude was linearly extrapolated to the logarithmic disparity axis. The difference between the evoked potential estimate and the psychophysical threshold was less than 20% in 56% of all cases; 16 of 18 cases (89%) agreed within a factor of 2.

Depth Perception

Automatic infrared refractors--a comparative study.

In order to determine the performance of seven automatic infrared (IR) eye refractors, measurements have been conducted on a model eye as well as on normal subjects and patients under standardized conditions. Concerning the model eye, a range of measurement slightly smaller than specified and linearity errors have been detected on several instruments. Using a group of 55 normal ametropic subjects, the results of the automatic refraction were compared to the results of a conventional subjective examination. The spherical equivalent differed by less than 0.51 D in more than 80% of all cases on all instruments. The error of the cylinder power was smaller than 0.51 D in more than 90% of all cases. Larger errors were found on patients with intra ocular lenses, aphakic eyes, or scattering eye media. In each of these groups the automatic refraction was at times either impossible or yielded a wrong result.

Aphakia

Retinal visual acuity with pattern VEP normal subjects and reproducibility.

Retinal visual acuity was determined using an objective method by means of VEP. Sixty subjects with subjective visual acuity ranging from 20/25 to 20/12 were measured with a computer-controlled laser interferometer, using interference fringes modulated in counterphase as pattern-reversal stimulus. Steady-state VEPs taken at different spatial frequencies were evaluated by Fourier analysis and linear regression. Objective retinal acuity was calculated as the intersection between the linear regression line and the linear scaled spatial frequency axis. In 68% of all cases, the difference between objective and subjective visual acuity was less than +/- 5.1 cycles per degree (c/d) (+/- 15.4% referred to subjective acuity); in 86%, the difference was less than +/- 7.7 c/d (+/- 23.1%).

Evoked Potentials, Visual

Modern instruments for subjective refraction. A comparative study.

In a comparative study, subjective refractions were carried out on 115 normal subjects and 118 patients with four instruments (Humphrey Vision Analyzer, Möller/CooperVision Visutron, Reichert SR-IV, Rodenstock/Coburn Phoromat). The accuracy of the prescriptions obtained, the visual acuity results and the measurement time required were analyzed. The highest accuracy was obtained with the remote-controlled phoropters Phoromat and Visutron. The fastest instrument was the SR-IV.

Cataract

A new laser interferometer for the stimulation of pattern-reversal visual evoked potentials.

A laser interferometer for visual-evoked-potential (VEP) stimulation that allows a rapid pattern reversal in less than 0.5 ms and a continuous variation of the spatial frequency of the pattern is described. The interferometer is applied for the assessment of objective visual acuity. The Fourier transform of the VEP response to the rapid pattern reversal shows an extremely small bandwidth.

Evoked Potentials, Visual

Binocular micromovement recording of human eyes:--methods.

An apparatus is described for binocular recording of micromovements of human eyes, with the contact lens mirror technique used frequently. Horizontal and vertical micromovements of both eyes are recorded simultaneously by means of instruments measuring light-spot positions. Spatial resolution of the measuring instrument is 12 s of arc, while the time resolution is 0.5 ms for this apparatus. The fixation mark (Snellen character) is at a distance of 5 m without any restrictions to the view. The micromovement data are registered by a microcomputer. A second computer enables precise calculation of the results which are given as multicoloured graphs (X/Y-, X/t-graph, velocity/t-graph, frequency analysis, phase correlation of both eyes). In this way the requirements are met for the clinical application of this measuring instrument on patients.

Contact Lenses

[The strength of retinal sear tissue after argon-, xenon-, and cryocoagulation (author's transl)].

A method is presented for measuring the adhesive force between retina and pigment epithelium in rabbit eye preparations. This force shows a great interindividual variation and so the absolute value is of little interest. The variation of several readings in one animal is, however, significantly smaller. Therefore the relative change in adhesive force between untreated and treated areas of one animal can be used. The data shows similar increases of +25-30%. For laser-, xenon-, and cryocoagulation, the maximum adhesive strength is reached within 4-8 days after treatment. After a few weeks the additional adhesion in treated areas slowly fell to about +10% as compared to pretreatment levels.

Adhesiveness

Neural contrast sensitivity measurements with a laser interference system for clinical and screening application.

A He-Ne laser interference device for clinical measurements of the neural contrast sensitivity fraction (CSF) is described. The system uses planoparallel glass plates for the generation of sinusoidal gratings on the retina. Incoherent light from two light-emitting diodes is superimposed for reducing the contrast to a range from 2 X 10-3 to 0.5. The device is coupled with a microcomputer that calculates the contrast sensitivity (CS) and permits control of the system by a dialogue program. The mean CSF for normal observers (95 eyes) is presented, and the data are compared with overall CS values from other authors. Pathologic CSFs of three patients are shown as examples for the clinical application. For screening purposes the CS values for five spatial frequencies are compared with the normal CSF. And loss-of-contrast values are calculated.

Equipment Safety

[Importance of orientation on visual and neuronal resolution (author's transl)].

Spatial resolution of gratings of different orientation has been evaluated in 39 patients, by means of projected slides and by laser interference fringes. Both methods reveal a significantly higher resolution (about 12%) for vertical or horizontal bars compared to oblique ones. The similarity of findings for both methods is indicative of the neuronal origin of this orientation-dependent effect and exclude an optical origin.

Adolescent

[Optical properties and clinical performance of the Topcon retinal camera TRC-FET 3 (author's transl)].

A Topcon Retinal Camera TRC-FET 3 was tested for several weeks under laboratory and clinical routine conditions. The optical properties turned out as good and meet the requirements for retinal cameras in all details. Remarkable is the high speed of the imaging system, which allows working with low flash energies and with high flash frequency. The resolving power is high (8 microns at the center) and decreases slowly to the periphery. The illumination of the fundus is fairly homogeneous. Many filters of good performance are provided. There was no problem in handling the camera, and the test photographs as well as the clinical routine photographs were of high quality.

Clinical Trials as Topic

[Laser lesions of the anterior segment of the rabbit eye. Studies of cornea, iris, lens and sclera using an argon, ruby and YAG-laser].

The anterior segment of the eye of 100 rabbits was exposed to radiation from Argon, Ruby and YAG lasers. The healing process of liminal and supraliminal lesions was evaluated histologically. The iris is the most sensitive tissue for all three lasers (threshold radiant exposure, Argon: 0.004 kJ/cm2, Ruby: 0.01 kJ/cm2, YAG: 0.05 kJ/cm2). Higher energies necessary for lesions in the transparent media were available only with the Argon and YAG lasers. The Argon laser failed to produce lesions in the lens, and threshold radiant exposure for the central cornea was 8 kJ/cm2. The thresholds for YAG lasers lesions in the central cornea and lens were of the same magnitude. The marked absorption of energy by the iris may lead to an accumulation of heat, resulting in lesions of adjacent tissues or a reduction of threshold for those lesions: The threshold in the peripheral cornea was ten times lower than in the center with the Argon laser, four times lower for the YAG laser. The thresholds for Argon and YAG laser lesions in the peripheral sclera lay between the thresholds for the iris and for the peripheral cornea. A safety standard for the anterior segment of the eye should be based on the lowest threshold (Argon laser lesion of the iris). The application of a factor 100 results in a maximal permissible exposure of 40 mJ/cm2. The influence of the beam diameter could not be evaluated.

Animals