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Biomedical subjects

B Petchclai

Publications and source records attributed to B Petchclai.

At least 55 records · Page 3Linked to original sources
PubMed1980

Application of thin layer immunoassay (TIA) for demonstration of antibodies against Entamoeba histolytica.

Thin layer immunoassay (TIA) was used to demonstrate antibodies against Entamoeba histolytica in sera from patients and blood donors. The TIA results agreed well with those obtained by the indirect hemagglutination (IHA) and immunodiffusion (ID) techniques. It is suggested that because of its technical simplicity and low cost TIA may be used alternatively or in addition to the IHA and ID techniques for screening patient sera for antibodies to E. histolytica. However, the new technique must first be evaluated on a goup of clinically well defined patients with different stages of amebiasis.

Amebiasis↗
PubMed1980

Cell-mediated immune response in amebiasis.

Studies on cell-mediated immune response (CMIR) to amebia antigen in amebic liver abscess showed conflicting results. The leucocyte migration test was employed in nine cases of amebic liver abscess and CMIR to amebic antigen was demonstrated in four cases. The role of CMIR in the pathogenesis of amebic liver abscess remains unknown but its presence is confirmed.

Adult↗
PubMed1980

The value of an indirect hemagglutination test for streptococcal antibodies in children with acute rheumatic fever.

This study was made to evaluate the value of the IHA test for streptococcal antibodies in children with acute rheumatic fever in comparison with the conventional ASO titer. The upper limit titers of ASO and IHA in control group were 170 and 640 respectively. All sera from 37 patients with rheumatic fever had IHA titers over 640. In contrast, the level of ASO titer above upper normal limits was found in only 62% of these cases. This report present data which favours the use of IHA test. Its sensitivity is very similar to that of the commercial Anti Streptozyme test. In addition, the test is simple and could be performed economically. More studies are needed to evaluate the test further. If the results proved as favourable as this report a negative test would be of a considerable value in excluding the diagnosis of acute, rheumatic fever especially in questionable cases.

Acute Disease↗
PubMed1978

Diphtheria and tetanus antitoxin levels in Thai children.

Determination of diphtheria and tetanus antitoxin levels by an indirect haemagglutination method were conducted in 101 nonimmunized schoolchildren, 155 pediatric patients and 102 blood donors. Diphtheria and tetanus antitoxin levels were found mostly adequate among immunized children. Diphtheria antitoxin levels were found adequate in 68.3% of the non-immunized schoolchildren. Tetanus antitoxin levels were found inadequate for protection in the non-immunized children and adults. Immunization of children and adults with diphtheria and tetanus toxoid are highly recommended.

Adult↗
PubMed1977

The association between thalassaemic diseases and traits and post-streptococcal acute glomerulonephritis.

This prospective study showed that thalassaemic patients, though susceptible to streptococcal infection, as evident from their elevated A.S.O. titres, are not, as has been suggested, more likely to develop acute glomerulonephritis than the rest of the population. The incidence of thalassaemic trait found among typical cases of acute glomerulonephritis does not exceed that of the general Thai population. There were 5 out of 56 cases of thalassaemic patients having transient microscopic haematuria.

Acute Disease↗
PubMed1975

A preserved leukocyte preparation for quality control.

A preparation technic for stabilized leukocytes for quality control is described. Human leukocytes are separated from 5 ml. of blood using dextran; the erythrocytes are lysed with isotonic NH4Cl-Tris buffer, pH 7.2. The washed leukocytes are resuspended in 50 ml. of cacodylate HCl buffer, pH 7.4. Five ml. of 2.5% glutaraldehyde were slowly added, and preservation is accomplished by storage overnight at 4 C. The washed leukocytes are resuspended in physiologic saline solution containing 1:1,000 sodium azide, then stored at 4 C and at room temperature. The preparations were highly stable at both temperatures for at least 3 months, as indicated by both manjal and electronic leukocyte counts.

Azides↗
PubMed1975

Leukocyte migration test in a modified agarose medium.

The medium used for the leukocyte migration test contained 1% agarose, 0.003 M HEPES, TC medium 199, and 10% horse serum. The pH was adjusted to 7.3 with 2 N NaOH. The leukocyte migration test in this medium correlated with the intradermal test in 36 subjects when PPD was used as antigen, and the results were comparable to those of the reference technic. Addition of HEPES to the original medium enables the test to be performed in a standard incubator.

Cell Migration Inhibition↗