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Biomedical subjects

B Pedersen

Publications and source records attributed to B Pedersen.

At least 199 records · Page 11Linked to original sources
PubMed1983

Requirements for transport of HSV-1 glycoproteins to the cell surface membrane of human fibroblasts and Vero cells.

The intracellular transport of the HSV-1 glycoproteins gA/gB, gC and gD has been followed by the indirect immunofluorescence technique (IIF). Infected tissue culture cells were stained with monoclonal antibodies made to the individual glycoproteins and with fluorochrome-coupled wheat germ agglutinin reacting specifically with Golgi apparatus of the cells. Staining of either infected, human fibroblasts or of VERO cells at 9 hours p.i. with antibodies to gA/gB showed a prominent ring-like nuclear fluorescence and distinct staining of the Golgi apparatus in the cells. Antibodies to gC and gD stained mainly the Golgi apparatus and areas close to or at the surface of the cells. By immunocytolysis of HSV-1-infected VERO cells the viral glycoproteins were demonstrable at the surface of cells but growth of infected cells in the presence of either TM or monensin inhibited the expression of most of the viral glycoproteins at the cell surface. Blocking of the glycosylation of the viral glycoproteins with tunicamycin (TM) was followed by accumulation of the core of the glycoproteins gA/gB and gD in granular structures close to the nucleus as seen by immunofluorescence microscopy. Antibodies to gC did also stain granules close to the nucleus but in addition the periphery of the cells were stained. Inhibition of intracellular transport from the Golgi apparatus by the carboxylic ionophore monensin was followed by accumulation of all the HSV-1 glycoproteins in vesicles derived from the Golgi apparatus in both human fibroblasts and VERO cells. Our data thus support the hypothesis that the HSV-1 glycoproteins are processed in the Golgi apparatus before the transport to and incorporation into the cell surface membrane of infected cells and into virion envelopes.

Animals↗
PubMed1983

Thromboembolic prophylaxis with dihydroergotamine-heparin in abdominal surgery. A controlled, randomized study.

The prophylactic affect of dihydroergotamine-heparin (0.5 mg dihydroergotamine plus 5,000 units of heparin administered twice daily) against postoperative thromboembolic complications after gastrointestinal surgery was compared with the affect of 5,000 units of heparin administered three times daily in a controlled, randomized study. Fifty patients received the dihydroergotamine and heparin combination twice daily and 50 patients received 5,000 units of heparin three times daily. The two groups were comparable with respect to age, sex, body weight, and type of operation. The presence or absence of deep venous thrombosis in the legs was determined postoperatively by the radiofibrinogen uptake test. The frequency of deep venous thrombosis in the dihydroergotamine and heparin group was 12 percent compared with 18 percent in the heparin group (0.1 greater than p greater than 0.05). Wound hematoma developed in two patients in the heparin group and in one patient in the dihydroergotamine and heparin group (4 and 2 percent, respectively). No other hemorrhagic complications occurred. By combining heparin with dihydroergotamine it is possible to reduce the daily heparin dose by one third and still obtain the same level of prophylaxis against thromboembolism. The frequency of bleeding complications was very low in both groups, since only one wound hematoma occurred in the dihydroergotamine and heparin group and two in the heparin group.

Abdomen↗
PubMed1983

Extreme hypodiploidy in a case of myelomonocytic crisis of chronic myelogenous leukemia.

Two cytogenetically distinct populations of marrow cells were observed in a 28-year-old woman who developed a fulminant blastic crisis (BC) of chronic myelogenous leukemia (CML) after only 1 year in the chronic phase: one population with 35-36 chromosomes, the other showing 66-72 chromosomes. Cytochemical investigation demonstrated a myelomonocytic type of BC. Chromosome banding and correlation analysis of the mean karyotypes of the two populations showed that a close relationship existed between them, indicating that one population had developed from the other. The cytogenetic evidence suggests but does not prove that the cells with triploid chromosome numbers developed from the extremely hypodiploid population by duplication of the chromosome complement. The extreme cytogenetic diversity of both populations indicates that each was undergoing further cytogenetic evolution.

Adult↗
PubMed1983

The influence of dietary tea, coffee and cocoa on protein and energy utilization of soya-bean meal and barley in rats.

Two series of balance experiments were performed with growing rats to test the effect of black tea, green tea, coffee and cocoa on protein and energy utilization. In Expt 1 soya-bean meal was fed as a basal diet and supplemented with freeze-dried materials from 11 black tea, green tea or coffee/500 g dry matter. Cocoa powder, corresponding to 11 of the beverage, was also added to the basal diet. In Expt 2 the procedure was repeated with a barley-based diet. In both experiments both tea varieties and coffee had significantly negative effects on true protein digestibility and biological value, while digestible energy was only slightly affected in the barley-based diet. Cocoa had no effect on protein or energy utilization in either soya-bean meal or barley diets, although the protein in cocoa powder was completely indigestible. As the tannin concentration in both tea varieties and coffee was very high it is assumed that the observed deleterious effects might, in part, be explained by anti-nutritional effects of tannin. The strongest deleterious effect was recorded for black tea.

Amino Acids↗
PubMed1983

Accumulation of herpes simplex virus type 1 glycoprotein D in adhesion areas of infected cells.

By indirect immunofluorescence microscopy (IIF) we have localized glycoprotein D (gD) of herpes simplex virus-infected cells to the vinculin-containing junctional areas and to the focal adhesion sites on the ventral surface of the cells. Double-staining IIF showed that gD and vinculin co-distributed in the infected cells and treatment of the infected cells 4 h post-infection with rabbit antibodies to gD prevented attachment of the cells to the growth substratum. Our results therefore support the hypothesis that gD plays a central role in the social behaviour of infected cells.

Biological Transport↗
PubMed1983

Acute valproate intoxication: biochemical investigations and hemodialysis treatment.

A 20-year-old female was comatose for several days after intoxication with 75 g sodium valproate (VPA). She was successfully treated with hemodialysis, hemoperfusion, i.v. infusion of glucose, and given intensive care. Although a peak serum VPA of 2120 micrograms/ml (14720 microM) was registered 8 1/2 h after drug intake, no definite drug-related hepatotoxic or thrombocytopenic effects were seen. The only signs of organotoxicity were slight increases in serum amylase and a transient proteinuria. Further acute biochemical alterations were a decrease in serum calcium, an increase in plasma ammonia as well as in serum propionate, and elevated urinary excretions of adipic and suberic acid. Analysis of amino acids in serum revealed a stable increased level of glycine.

Adult↗
PubMed1983

Treatment of epilepsy in women of child-bearing age: patients' opinion of teratogenic potential of valproate.

83 epileptic women of child-bearing age in treatment with valproate were informed of the potential teratogenic effect of valproate and the precautions available to avoid the suspected risk of malformation i.e. neural tube defect. 66% of the women wished and were able to be pregnant. Out of these 51% at present preferred to continue with valproate. 9% of the patients were immediately switched to other antiepileptic drugs and the remaining part had not yet made up their minds. The study demonstrates that following the statement from the Danish Health Authorities it is difficult also to pay attention to the individual wishes of the women.

Adolescent↗
PubMed1982

Liquor:serum quotients of IgG and albumin in patients with meningism, meningitis and multiple sclerosis.

Liquor:serum quotients of IgG and albumin in patients with meningism, meningitis and multiple sclerosis have been studied. Abnormal quotients indicative of disturbed blood-brain barrier function and/or intrathecal IgG synthesis were found in various proportions of all 3 groups of patients. In some patients with aseptic meningitis and meningism, the abnormal quotients were comparable with those found in patients with multiple sclerosis. The reason for this is unknown. An unspecific reaction to febrile illness may be involved. As a consequence, the findings of abnormal liquor:serum quotients of IgG and albumin should be evaluated with great caution in patients with recent or present febrile illness.

Adolescent↗
PubMed1982

Effect of tunicamycin on the synthesis of herpes simplex virus type 1 glycoproteins and their expression on the cell surface.

Herpes simplex virus specifies five glycoproteins which have been found on the surface of both the intact, infected cells and the virion envelope. In the presence of the drug tunicamycin, glycosylation of the herpes simplex virus type 1 glycoproteins is inhibited. We present in this report evidence that the immunologically specificity of the glycoproteins designated gA, gB, and gD resides mainly in the underglycosylated "core" proteins, as demonstrated by the immunoblotting technique. We showed also that tunicamycin prevented exposure of the viral glycoproteins on the cell surface, as the individual glycoproteins lost their ability to participate as targets for the specific antibodies applied in the antibody-dependent, cell-mediated cytotoxicity test. Immunocytolysis was reduced between 73 and 97%, depending on the specificity of the antibodies used. The intracellular processing of the herpes simplex virus type 1-specific glycoprotein designated gC differed from the processing of gA, gB, and GD, as evidenced by the identification of an underglycosylated but immunochemically modified form of gC on the surface of infected cells grown in the presence of tunicamycin.

Animals↗
PubMed1982

Secretory IgA specific for herpes simplex virus in lacrimal fluid from patients with herpes keratitis--a possible diagnostic parameter.

In the present study a solid-phase radioimmune assay was used for the demonstration of herpes simplex virus-specific IgG and secretory IgA antibodies in the lacrimal fluid from patients with active recurrent herpes keratitis. The method was quantitative and made it possible to test specifically for the production of secretory IgA antibodies produced during an active herpes simplex virus infection. The production of secretory IgA was followed in 2 patients with fresh recurrent lesions. The HSV-specific secretory IgA could be demonstrated during the first 10 days of infection, where the maximal concentration was reached 3-5 days after the first symptoms occurred. The secretory antibodies were locally produced, and it is shown for the first time that herpes virus-specific secretory antibodies were of diagnostic value.

Adult↗
PubMed1982

Clinical measurement of hearing aid insertion gain.

The probe microphone devised by Lauridsen & Günthersen (1981) was tested in a clinical situation, and the results were compared with those obtained with a miniature electret microphone place in the ear canal (cf. Harford, 1980), and also with those obtained with the eardrum microphone on the acoustic manikin KEMAR. Good agreement was found between all three methods. Investigation of the insertion gain in 82 ears, fitted with hearing aids according to the classical criteria, revealed a reduction in the insertion gain in the higher frequency bands, starting from an average of 2 kHz.

Adult↗