Pan- and auto-agglutination in albumin: a serological and immunochemical study of five cases.
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Biomedical subjects
Publications and source records attributed to B P Moore.
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Anti-Sd-a is difficult to identify, unless a special technique and strongly reacting Sd(a+) red blood cells are used. Sd-a has a broad distribution of antigen strength. The strongest examples [Sd(a++) or "Super Sid" and strong Sd(a+)] may be readily identified by using an extract from the seeds of Salvia horminum.
Three examples of an antibody defining a 'new' high frequency red blood cell antigen, Era, are described. Family studies show Era to be inherited, but the mode of inheritance may not be straightforward.
A lectin reacting with T, Tn, and Th polyagglutinable red blood cells was found in an extract from the seeds of Hibiscus sabdariffa (Sorrel). This lectin is thus a useful addition to those available for distinguishing various types of red blood cell polyagglutinability.
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A combined hepatitis test (Ausria/Core, Abbott Laboratories, Wiesbaden, West Germany) detects hepatitis B surface antigen by radioimmunoassay and antibody to the core antigen by enzyme immunoassay using a single bead. It was found to have comparable sensitivity to commercially available tests from the same manufacturer for each component. Reproducibility was satisfactory for both components. The combined test is simpler and cheaper to perform than two separate tests.
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Manual hexadimethrine bromide (Polybrene) tests (Polybrene in low-ionic medium) were used in parallel with manual low-ionic-strength solution (LISS) procedures for the routine testing of patient samples referred to a general hospital blood bank. Of 5646 consecutive sera tested, 5167 (91.5%) did not react with either technique; 320 sera (5.7%) reacted in both methods. The Polybrene technique detected 63 antibodies which did not react in the LISS methods. One hundred sera did not react in the Polybrene test, but did react in the LISS methods. Sera showing discrepant results between the two methods were further tested in a reference laboratory. Polybrene tests appeared to be better in avoiding reactions due to clinically nonsignificant antibodies. The LISS methods, however, appeared to be more sensitive in detecting antibodies of potential clinical significance.
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