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Biomedical subjects

B Lee

Publications and source records attributed to B Lee.

At least 433 records · Page 24Linked to original sources
PubMed1980

Investigation of the influence of progesterone on mouse embryo transport by using antiprogestational steroids.

The rate of embryo transport through the mouse oviduct was unaltered by ovariectomy after ovulation, essentially unaltered by administration of progesterone after ovulation but increased by preovulatory administration of progesterone. The antiprogestational steroid RMI 12,936 caused an arrest of embryo movement when given on Day 1, 2 or 3 of pregnancy and similar, though less marked, delay was caused by R2323, another progesterone antagonist. The effects of RMI 12,936 given on Day 1 were reversed by progesterone administration after a latent period of 24-48 h. These results indicate that the egg transport process in the mouse in triggered by progesterone and requires continued progesterone activity for its maintenance.

Androstenols↗
PubMed1979

The effect of reserpine, oestradiol and in-vitro perfusion on oviduct calcium levels in the mouse during egg transport.

Mouse oviduct calcium content, determined by atomic absorbance after ashing of the tissue, showed a significant fall on Day 2 of pregnancy followed by a significant rise on Day 3. This pattern was altered by administration of reserpine and oestradiol in doses which were shown to alter the rate of egg transport. In-vitro perfusion of the oviduct, capable of maintaining muscular activity and back and forth movement of eggs for 24 h, was associated with lack of forward progressive motion of eggs and by a more rapid increase in tissue calcium levels during incubation than occurred in vivo.

Animals↗
PubMed1978

Human uracil DNA N-glycosidase: studies in normal and repair defective cultured fibroblasts.

Uracil DNA N-glycosidase, an enzyme which participates in the excision of uracil from DNA, was measured in extracts from fibroblasts lines cultured from normal subjects, from several subjects with the genetic disease xeroderma pigmentosum, and from a subject with ataxia telangiectasia. The cell lines representative of complementation groups A and D of xeroderma pigmentosum and of ataxia telangiectasia had roughly the same level of activity as did the normal cells. On the other hand, cells from two xeroderma pigmentosum variants (XP4BE and XP13BE) had roughly half the normal level of activity, and cells from the heterozygous mother of XP4BE had an intermediate level of activity. In spite of these quantitative differences, no systematic alterations in reaction characteristics, apparent Km for substrate, or purification characteristics were noted for enzyme from any of the lines. Thus a causal relationship, if any, between levels of activity and the disease symptoms is equivocal.

Apurinic Acid↗
PubMed1978

Xeroderma pigmentosum fibroblasts of the D group lack an apurinic DNA endonuclease species with a low apparent Km.

Apurinic DNA endonuclease activity from cultured human fibroblasts was resolved into two species by phosphocellulose chromatography. The species had sedimentation coefficients of 3.3 S and 2.8 S and apparent Km's for apurinic sites of 5 and 44 nM, respectively. The low Km species was absent from extracts of cell lines XP5BE, XP6BE and XP7BE of xeroderma pigmentosum complementation group D.

Apurinic Acid↗
PubMed1978

Food and Drug Administration's adverse drug reaction monitoring program.

The adverse drug reaction monitoring program of the Division of Drug Experience within the FDA is described. Historical information on the development and activities of the current drug reaction monitoring program, and goals and objectives of the current program are discussed. Also presented are a brief description of the Voluntary Reporting System, intensive drug monitoring studies and special epidemiologic studies, and a workable definition of alert reports and examples of their previous role within the FDA. Pharmacists should participate actively in adverse drug reaction monitoring.

Drug Industry↗
PubMed1977

Drug reaction alerts.

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Drug-Related Side Effects and Adverse Reactions↗
PubMed1975

Crystallographic studies on L-asparaginase from Proteus vulgaris. II. Symmetry and location of the tetrameric molecule.

Analyses of the x-ray diffraction intensity data by the Patterson synthesis and rotation function techniques show that the true space group of the monoclinic crystals of L-asparaginase (L-asparagine amidohydrolase, EC 3.5.1.1) from Proteus vulgaris is P21, that the molecular centers lie at x = 0.054, y = 0, z = 0.256, and its symmetry related positions, and that the tetramer molecules possess three approximate, mutually perpendicular 2-fold rotational symmetries, the axes of which run along the directions of the crystallographic a*-, b-, and c-axes. In addition, an investigation of the molecular packing arrangement in the crystal indicates that the tetramer molecules possess an approximately regular tetrahedral subunit structure.

Asparaginase↗