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B Kantelip

Publications and source records attributed to B Kantelip.

116 records · Page 7Linked to original sources
PubMedDate not supplied

[Ultrastructure of bronchial biopsies obtained by fibroscopy during a remission between crises in asthmatic adults].

This study describes the ultrastructure of bronchial biopsies from ten patients with bronchial asthma in remission. The superficial submucosa was the seat of a cellular infiltration: the plasma-cells are the most numerous. Light microscopy shows thickening of bronchial basement membrane. By electron microscopy, the basement membrane appears intact and the thickness consists of collagen fibrils in plexiform arrangement. The epithelium shows goblet cells and basal cells hyperplasia and lesions of the ciliated cells. The defects in the ciliary ultrastructure are: compound cilia, abnormal cilia and internalized cilia. They can be regarded as non specific lesions. Two types of cells infrequent in the bronchial mucosa are found: one characterized by apparition of a dictyosome with mucous secretion; the second by defects of the cellular membrane and little electron dense cytoplasm. These two types of cells show a "mitochondrial barrier" like the ciliated cells. This study reveals that numerous lesions are seen in the bronchial mucosal of patients with severe asthma even in remission.

Adult↗
PubMedDate not supplied

Cellular localisations of interleukin-1 beta, interleukin-6 and tumor necrosis factor-alpha mRNA in a parasitic granulomatous disease of the liver, alveolar echinococcosis.

Alveolar echinococcosis (AE), an uncommon and very severe parasitic liver disease, can be considered as an "infectious model" of granulomatous disease, where cellular immunity plays a key role in the defence against Echinococcus multilocularis, the larval cestode responsible for the disease. We analysed the localisation of the expression of the pro-inflammatory cytokines IL-1 beta, IL-6 and TNF-alpha mRNA in human AE liver lesions, in the periparasitic granulomas and in the hepatic parenchyma, as well as the phenotypic characteristics of the cells on serial sections. In situ hybridizations, using anti-sense 35S dUTP-labeled IL-1 beta, IL-6 and TNF-alpha riboprobes, were performed on cryostat liver sections; the sense probes were used as negative controls. IL-1 beta, IL-6 and TNF-alpha mRNA were observed in macrophages located at the extreme periphery of the granuloma, between the lymphocytic ring and the liver parenchyma, in patients with active AE. No cytokine mRNA expression was observed in a patient with an abortive case. Only TNF-alpha mRNA was located in the periparasitic area, in cells morphologically identified as macrophages but exhibiting an unusual phenotype (CD 11b-, CD 25+); this particular expression was observed only in those patients with very fertile lesions, associated with centro-granulomatous necrosis. These results show that pro-inflammatory cytokines are consistently produced by macrophages at the periphery of the periparasitic granuloma and can serve as mediators of acute-phase protein secretion and of fibrogenesis in that location.(ABSTRACT TRUNCATED AT 250 WORDS)

Adult↗