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B Kaijser

Publications and source records attributed to B Kaijser.

At least 145 records · Page 8Linked to original sources
PubMed1977

Protective capacity of antibodies against Escherichia coli and K antigens.

Antibodies to Escherichia coli O and K antigens were raised in rabbits by repeated immunizations with whole, Formalin-killed and, later, liver bacteria. The serum antibody levels were determined with the ammonium sulfate precipitation technique after radioiodinating the antigens. The K antigens had to be conjugated to proteins before labeling. Such conjugations were performed using cyanogen bromide for the K1 antigen and bisdiazobenzidine for the K13 antigen. The protective capacities of the rabbit antisera were tested in intraperitoneally infected mice. The protective capacity of the antisera was expressed per ammonium sulfate precipitation titer. The results showed a significantly higher protective effect for the antibodies against the K1 and K13 antigens than for the antibodies against the O2 and O6 lipopolysaccharides.

Animals↗
PubMed1976

Serum levels of immunoglobulin and complement in giant-cell arteritis.

The levels of IgG, total complement, and complement factors C3 and C4 were elevated in serum from patients with giant-cell arteritis as compared to a control group of patients in the same range of age. No increase of the IgM concentration was found. The concentration of IgA was higher in men with giant-cell arteritis as compared to men in a control group. This increase of IgA was not seen in women. There were no statistically significant differences in any of the studied variables between those with a histologically verified arteritis on temporal biopsy specimens and those without.

Aged↗
PubMed1976

The local immune response to Escherichia coli O and K antigen in experimental pyelonephritis.

Although the systemic and local immune response to the O antigen of Escherichia coli has been well characterized, little information is available on the immune response to K anigen. Experimental hematogenous pyelonephritis was produced with Escherichia coli 06 K13 H1 and the serum and local (intrarenal) antibody response to O and K antigens was determined with the enzyme-linked immunosorbent assay. Both local and serum antibody responses to the K antigen were significantly less than that to the O antigen. The K antigen induced low titer IgM and IgG antibody responses in fewer than one-half of the animals and did not induce a local IgA response in any animal. In contrast, the O antigen induced local antibody responses in each of the immunoglobulin classes in all animals from day 9 of infection. Similarly, the serum IgM and IgG antibody titers to the K antigen were significantly less than those evoked in response to the O component of the Escherichia coli. No serum IgA anti-K antibodies were detected. These observations helf clarify the roles of these two antigens in pyelonephritis. Although the K antigen of Escherichia coli functions as a virulence factor in upper urinary tract infections, this antigen does not elicit a significant immune response, whereas the O antigen does induce a significant antibody response which could be of protective or diagnostic benefit.

ABO Blood-Group System↗
PubMed1976

Local immunie response in experimental pyelonephritis in the rabbit. III. Lymphocyte responsivieness to O and K antigens of Escherichia coli.

The response of circulating and infected kidney lymphocytes to the O (lipopolysaccharide) and K (polysaccharide) antigens of an Escherichia coli O6 K 13 H1 strain was determined. Both circulating and kidney lymphocytes showed significant incorporation of [3H-methyl]thymidine into DNA when incubated with the O antigen, whereas neither responded to the K antigen. The lipid moiety of the lipopolysaccharide was required for lymphocyte responsiveness. Upon sequential incubation of O antigen and fluoresceinated homologous antiserum, 24-30 per cent of kidney lymphocytes were shown to have surface receptors for O antigen, whereas none had surface receptors for K antigen. Although the K antigen is an important determinant of invasiveness of the upper urinary tract, it fails to elicit a cellular immune response or attach to lymphocytes from the infected kidney in experimental pyelonephritis.

Animals↗
PubMed1975

[Immune defects].

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Complement System Proteins↗
PubMed1975

Antazoline-induced immune hemolytic anemia, hemoglobinuria, and acute renal failure.

A case of repeated antazoline-induced immune hemolytic anemia, thrombocytopenia, hemoglobinuria, and acute renal failure is reported. The first episode of renal failure occurred after an i.v. pyelography causing an anaphylactic shock, and the two later episodes were preceded by allergic reaction to drugs. Antazoline was given among other remedies, but this drug was the only one used for treatment on every occasion. The clinical picture and the immunological tests, including an antazoline-dependent Coombs' test, indicate that the blood disorders might have been caused by a type 2 allergic reaction and renal lesion by a type 3 reaction, at least on the second and third occasions.

Acute Kidney Injury↗
PubMed1975

Asymptomatic bacteriuria in school girls. IV Difficulties of level diagnosis and the possible relation to the character of infecting bacteria.

As no method for localization of urinary tract infection has been shown to be absolutely reliable, six methods have been run parallelly in a study of 60 school girls with asymptomatic bacteriuria caused by E. coli. A poor correlation was obtained between "high infection" indicated by bladder washout test and abnormal findings of C-reactive protein, sedimentation rate, antibody titres or renal concentrating capacity, while findings of parenchymal reduction on the pyelogram and reflux on the urethrocystogram were often found within this group. The low frequency of abnormal findings of C-reactive protein, sedimentation rate and renal concentrating capacity in girls with a "high infection" may be explained by the finding that the ABU patients were infected with changed E. coli strains probably not able to cause the usual inflammatory reactions. The patients' bacteria were found to be less efficient in providing antigen for antibody determinations than were standard strains of same O group, indicating a difference between strains from ABU patients and standard strains. From the battery of tests used it seemed that most of the girls had a bladder infection. All tests were normal in 48% of the patients while at least three of the methods were abnormal in 12%.

Adult↗
PubMed1975

Immunological studies of an antigen common to many gram-negative bacteria with special reference to E. coli. Characterization and biological significance.

A number of different bacterial strains were analyzed for the presence of common antigens using immunodiffusion techniques. Most of the studied E. coli strains had many common antigens. Especially one antigen was found in all investigated E. coli as well as Proteus and Pseudomonas strains and antibodies to this antigen were also recognized in antimeningococcal antiserum. The antigen was found possibly to be at least partly a protein with low carbohydrate content located in the inner part of the cell wall and specific antiserum to it was produced by immunizing rabbits. Antiserum to the common antigen did not protect against intraperitoneal infection in mice, or against hematogenous pyelonephritis in rabbits. Irregularly in a few patients with pyelonephritis, a very low antibody response was indicated using indirect hemagglutination technique. A possible significance of the antigen for the future quantitation of antibodies to gram-negative bacteria is discussed, as well as of the specific antiserum for the diagnosis of gram-negative bacterial strains using immunodiffusion techniques.

Animals↗
PubMed1975

Experimental Escherichia coli O6 pyelonephritis in rabbits. Effect on O6 antibody quantity and avidity of prior immunization with E. coli O2 bacteria.

Haematogenous pyelonephritis was induced in rabbits using Escherichia coli 06:K13:H1 bacteria and the amounts and avidities of antibodies to the 06 antigen were analysed by the ammonium sulphate precipitation technique of Farr. In a group of six animals preimmunized with E. coli 02:K2ab:H1, five developed pyelonephritis and one pyelitis, as determined by histological examination. All aminals showed a considerable antibody response to E. coli 06 antigen during the infection. The animal with pyelitis gave a slightly smaller response than the others. The antibody avidity showed a pronounced variation. In a second group of six rabbits not preimmunized, five animals developed pyelonephritis. The titres of antibodies against E. coli 06 antigen increased during the infection inall of the six animals. However, the increase was significantly smaller than for the animals preimmunized with E. coli 02:K2ab:H1 (P smaller than 0.01). The pattern of the antibody avidities in this group was also heterogenous. The results are consistent with previous findings that exposure to serologically heterologous E. coli bacteria can enhance the development of the homologous antibody titres. This could be of relevance for serological diagnostic work as well as in the determination of the protective capacity of the antibody.

Ammonium Sulfate↗