Search PubMed⌕ Search

Biomedical subjects

B Kaijser

Publications and source records attributed to B Kaijser.

At least 91 records · Page 5Linked to original sources
PubMed1981

Chemoattractive factors from Escherichia coli: individual strain variation.

Thirty-five different Escherichia coli strains isolated from patients with asymptomatic bacteriuria, cystitis, or pyelonephritis were investigated regarding their capacity to stimulate leukocyte chemotaxis in vitro. There were significant differences between individual strains in degree of chemoattraction, as well as among individual leukocyte donors in their reactions to the same strains. These differences were not correlated to specific E. coli antibodies. No significant differences in chemoattraction were found among strains isolated from patients with asymptomatic bacteriuria, cystitis, or pyelonephritis. It was suggested that the production of chemoattractive factors was not important for the pathogenicity of different E. coli strains in relation to urinary tract infection.

Antigens, Bacterial↗
PubMed1981

Immunoelectgrophoretic pattern of Klebsiella O and K antigens.

Klebsiella capsular antigens K1-K72 and O antigens O1-O9 were isolated and rabbits immunized with corresponding bacteria. The immunoelectrophoretic patterns of the O and K antigens were studied. All K antigens analyzed were highly electrophoretically mobile, giving a marked precipitate of different lengths towards the anode. All the O antigens analyzed with the exception of O4 were nonmobile and gave a precipitate next to the start basin. The O4 precipitated towards the anode. These data indicate a possibility for using counterimmunoelectrophoresis in the diagnosis of Klebsiella antigen in body fluids or for capsular typing of the bacteria using the same technique.

Animals↗
PubMed1981

Studies on the K antibody response in rabbits immunized with a pool of five different K antigen-containing Escherichia coli.

The Escherichia coli K antibody response in rabbits which were immunized with a mixture of E. coli O1K1H7, O6K2a2cH1, O4K12, O6K13H1 and O6K53, or with only one of these strains, was analyzed using indirect hemagglutination. Some K antigens gave generally high antibody response, while others did not. There were not statistically significant difference concerning K antibody response for each of the K antigens when the rabbits were immunized with one strain or a mixture of strains. The information is important for the composition of a future E. coli vaccine containing several K antigens.

Animals↗
PubMed1981

Serum antibodies against Escherichia coli and Proteus mirabilis after different types of operations.

The serum antibody titer against Escherichia coli and Proteus mirabilis before and after different types of operations was studied in 105 adult patients. The different operations were: pyelo- or ureterolithotomi (32); ileal conduit urinary diversion (20); cholecystectomi (34); operation in the thyroid area (10); operation of varicose veins of the legs (9); and colectomies (10). High serum antibody titers against E. coli were found in 16% of the patients preoperatively. Antibody titres against P. mirabilis were high in 19% of the patients with urinary tract calculus but in none of those without urological disease. The antibody titre against E. coli and/or P. mirabilis increased significantly more often after operation in the urinary tract or colon than after the other operations. Increase of antibody titers were more often seen in patients who preoperatively had normal titres. A normalization of the titers was seen during the following 12 months and in most of those patients within the first 3 months.

Adolescent↗
PubMed1980

Enterotoxigenic Escherichia coli and other gram-negative bacteria of infantile diarrhea: surface antigens, hemagglutinins, colonization factor antigen, and loss of enterotoxigenicity.

Heat-labile enterotoxin (LT)-producing Escherichia coli and other enteric bacteria isolated from diarrheal Ethiopian children were studied for O and K antigen, production of heat-stable enterotoxin (ST), stability of LT production, properties of mannose-resistant hemagglutination (MRHA) (indicative of adhesive properties), and colonization factor antigen (CFA). Of the E. coli strains, 33% possessed O6, O8, or O78; 93% of these were stable producers of LT, and 86% produced both Lt and ST. O78 strains possessed CFA/I, whereas O6 and O8 strains possessed CFA/II. The E. coli with O antigens other than O6, O8, or O78, as well as the non-E. coli bacteria tended to lose their ability to produce LT; only 16% produced ST, and they only occasionally showed MRHA properties. The former group of E. coli strains might be considered as true enteropathogenic bacteria (enterovirulent E. coli), which may be identified serologically, while the pathogenic significance of the diversified latter group remains less certain.

Agglutination Tests↗
PubMed1980

Campylobacter fetus subspecies jejuni: a common cause of diarrhea in Sweden.

Stool samples from approximately 2,550 patients with gastrointestinal infections were cultured for bacterial pathogens between January 1978 and September 1979, and 277 campylobacter (10.9%), 183 salmonella, 89 shigella, and 17 yersinia infections were identified. Campylobacter was found in all age groups, with the majority in the age group of 20-34 years. Most cases of campylobacter diarrhea were isolated during summer or late fall. After five weeks, 90% of the patients had no Campylobacter in their stools. The incubation time was estimated to be one to six days. Campylobacter gastroenteritis, even more common than salmonella gastroenteritis, was in general not regarded as a serious disease, despite the fact that most patients had marked symptoms. Almost all patients recovered without antibiotic treatment, although in some cases the disease was long-lasting, and 50 of the 277 patients required hospitalization. Thus, Campylobacter must be considered in the diagnosis of patients with diarrhea.

Adolescent↗
PubMed1980

Comparison of counterimmunoelectrophoresis and the capsular reaction test for typing of pneumococci.

The capsular reaction test is recommended as the method of choice for typing of pneumococci. Counterimmunoelectrophoresis also works in most cases, but has the following definite drawbacks. (i) The polysaccharide type antigens of types 7F, 7A, 14, 33F, 33A, and 37 are neutral and therefore do not form precipitates when conventional buffers are used. (ii) Counterimmunoelectrophoresis does not permit differentiation between types within groups since many factor sera do not contain sufficient amounts of precipitating antibodies. Omniserum contains low-titered antibodies against cell wall antigens and also reacts with thioglycolate-containing broth.

Antigens, Bacterial↗
PubMed1980

Clostridium difficile in relation to enteric bacterial pathogens.

All feces samples (n = 2,390) sent to the Bacteriological Laboratory, Göteborg, Sweden over 43 days were, in addition to the standard procedure, cultivated to detect Clostridium difficile by using a special selective medium. C. difficile was found in 81 of the 2,390 samples (3%). These 81 samples represented 56 patients. Fifty of the 56 patients had diarrhea. In 20 of the 56 patients (36%), Salmonella, Campylobacter, or Yersinia were also found. Of the 2,390 samples 252 (11%) from 132 patients revealed positive isolations of Salmonella, Shigella, Campylobacter, or Yersinia in comparison to 3% for C. difficile alone. This result suggests that C. difficile can easily be isolated with proper techniques. Concomitant isolations of more than one bacterial pathogen in cases of gastroenteritis were often found for C. difficile. The theory presented here is that any change of the normal bacterial fecal flora due to such causes as antimicrobial treatment or enteric infections like Salmonella increases the possibilities of isolating C. difficile. The causative significance of C. difficile might in most cases be doubtful. The majority of cases with diarrhea and C. difficile were self-healing and not severe. Only 2 cases of 56 had severe diarrhea with extended engagement of the colonic mucous membrane, but with no signs of pseudomembranes.

Adolescent↗
PubMed1980

An experimental model for ascending acute pyelonephritis caused by Escherichia coli or proteus in rats.

Experimental, ascending acute pyelonephritis in rats was produced by injecting 0 x 5 ml of 10(9) bacteria/ml into the urinary bladder via the urethra. No traumatic manipulation of the ureters of kidneys was necessary. A grading system for kidney lesions based on macro- and microscopical examination was used. The capacity of different Escherichia coli and proteus strains to induce acute pyelonephritis was tested, and the E. coli 06K13H1 strain and the Proteus mirabilis 03H1 strain were especially capable of causing urinary tract infection. For the P. mirabilis 03H1 strain, a dominance of right kidney lesions was noted in contrast to the E. coli 06K13H1 strain which did not show any side preference.

Animals↗
PubMed1980

Cross-reactions between the Tamm-Horsfall glycoprotein and Escherichia coli.

Weak cross-reactions were demonstrated between the Tamm-Horsfall (TH) glycoprotein and antigens in the supernatants of boiled Escherichia coli R1-R4 strains using an inhibitory ELISA system. No cross-reactions could be determined between the purified R1-R4 lipopolysaccharides and the TH protein. Neither could any inhibition be recorded after trypsin treatment, indicating that the cross-reacting substance is protein in nature. The cross-reactions were verified by immunoprecipitation techniques. The biological relevance of the cross-reaction between E. coli and the TH protein particularly as an event inducing the formation of autoantibodies to the TH protein is discussed.

Antigens, Bacterial↗
PubMed1980

Effect of the properties of enzyme-antibody conjugates on the quantitation of serum antibodies to Escherichia coli 02 antigen by the enzyme-linked immunosorbent assay, Elisa.

Immunoglobulins conjugated to horseradish peroxidase (HRP) or to alkaline phosphatase (AP) were compared for determinations of antibodies to Escherichia coli 02 antigen using the enzyme-linked immunosorbent assay (Elisa). AP-IgG conjugates had a higher avidity for their antigen than HRP-IgG conjugates. The avidity of AP-IgG conjugates was higher than that of the native IgG fraction from which it was derived, whereas HRP-IgG conjugates had the same avidity as the native IgG fraction. The Elisa method was more sensitive when performed with AP-IgG conjugates. However, differences in antibody titer between sera from convalescent and acutely ill patients with urinary tract infection caused by E. coli were detected with a higher degree of accuracy with the Elisa-HRP method than with the Elisa-AP method. The differences between Elisa-AP and Elisa-HRP are discussed in relation to the qualities of the conjugates.

Absorption↗
PubMed1980

Intestinal vascular and central hemodynamic responses in the cat following i.v. infusion of live E coli bacteria.

Septic shock was induced in cats by i.v. infusion of live E. coli bacteria. The reactions of the series-coupled sections of the small intestinal vascular bed were followed continuously by a plethysmographic technique. Intestinal venous outflow was recorded using a drop counting technique. Intestinal venous outflow was recorded using a drop counting technique. The chest was opened and blood flow in the ascending aorta was followed electromagnetically. Pulmonary arterial blood pressure was recorded after direct cannulation. Following infusion of washed E. coli bacteria suspended in saline, a rapid early response was evident. This was characterized by arterial hypotension, unchanged aortic blood flow, pulmonary hypertension, and intestinal vasoconstriction. After 5-10 min a period of relative normalization followed. After 30-60 min a second phase, characterized by general circulatory deterioration, developed. The induced septic shock was initially characterized by a slight intestinal vasoconstriction. There was no accumulation of blood or fluid in the intestinal vascular bed; an isovolumetric state was thereby maintained in intestinal tissue throughout the experiments.

Animals↗
PubMed1980

Protective effect of immunization with Salmonella minnesota Re 595 against ascending Escherichia coli O6K13H1 pyelonephritis in rats.

Active as well as passive immunization with formalin-killed S. minnesota Re 595 bacteria protected against experimental, ascending pyelonephritis caused by E. coli O6K13H1 in rats. Absorption of the hyperimmune sera with Re antigen before passively given did not eliminate the protective effect. The specificity of protective antibodies is discussed.

Animals↗
PubMed1980

Hemodynamic effects of systemic or portal IV infusion of live E coli bacteria in the cat. A preliminary report.

The reactions in the series-coupled vascular sections of the small intestine and the changes in aortic blood flow, systemic arterial, and pulmonary arterial blood pressure were followed continuously in cats made septic by IV infusion of live E coli bacteria for 2 hours. Peripheral venous infusion initially induced systemic hypotension, pulmonary hypertension, and increased aortic blood flow, but decreased intestinal blood flow. These changes were normalized within 5-10 minutes. During the next 110 minutes systemic arterial blood pressure, aortic blood flow, and intestinal blood flow decreased continuously while intestinal blood flow resistance remained in the control range. Portal venous infusion induced a significantly less pronounced initial pulmonary arterial blood pressure increase. No initial intestinal vasoconstriction was noticed and intestinal blood flow resistance decreased during the bacterial infusion. In both series only small and insignificant changes of intestinal tissue volume were seen. The data suggest that the route of infusion is important to the response in experimentally-induced sepsis. The constant intestinal tissue volume argues against intestinal pooling as being of importance to the development of low blood pressure in septic shock.

Animals↗