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Biomedical subjects

B Jäger

Publications and source records attributed to B Jäger.

At least 37 records · Page 2Linked to original sources
PubMed1996

Differential expression of fructosyllysine-specific receptors on monocytes and macrophages and possible pathophysiological significance.

A differing individual expression of fructosyllysine-specific receptors has been found on the monocytes of 90 insulin-dependent diabetic patients and 101 healthy control subjects. The degree of receptor expression is neither age- nor sex-dependent; however, in the diabetic group it correlates significantly with the severity and age of onset of diabetic microangiopathy. To interpret the results of the human study, spontaneously diabetic and non-diabetic BB/OK rats were used to estimate tissue content of glucose-modified proteins and capillary basement membrane thickness in relation to the receptor expression on macrophages. In non-diabetic and diabetic rats no correlation was found between receptor expression and tissue content (i.e. artery, nerve) of fructosyllsine and fluorescent advanced glycation end products. However, animals which express the fructosyllysine receptor showed a greater increase in muscle capillary basement membrane thickness. There are indications that fructosyllysine receptor expression is positively associated with indices of diabetic complications such as microangiopathy and/or capillary basement membrane thickening.

Adult↗
PubMed1996

Psychotherapy and bulimia nervosa: evaluation and long-term follow-up of two conflict-orientated treatment conditions.

Analytic in-patient therapy (n = 32) and systemic out-patient therapy (n = 39) were applied to patients with bulimia nervosa, and the effects were evaluated 14, 26 and 38 months after the start of the treatments. Our assumptions about the general efficacy of both conflict-orientated techniques were confirmed: both therapies satisfactorily reduced the symptomatic behaviour, as well as secondary factors related to bulimia nervosa, in the long term. However, we could not identify differential effects of the two treatment regimes, which we had expected with regard to the very different therapeutic approaches. The similarities of outcome of both therapies predominate, with slightly better results in the case of the analytic in-patient treatment. The results are discussed with regard to the assumption that specific healing factors are involved in conflict-orientated treatments in addition to the "common factors' of psychotherapy.

Adolescent↗
PubMed1993

Synthesis and assembly of functionally active human vascular endothelial growth factor homodimers in insect cells.

Vascular endothelial growth factor (VEGF) is an angiogenic growth factor with a target-cell specificity highly restricted to vascular endothelial cells. Recombinant baculovirus were constructed for the production of two different forms of the human VEGF protein in insect cells. VEGF165 and VEGF121 proteins produced by Sf158 cells underwent a similar processing compared with mammalian cells, including efficient glycosylation, formation of a disulfide-linked dimer and secretion into the media. Only one of these forms, VEGF165 had a high affinity for heparin and this characteristic was used to purify this form to homogenicity by a two-step heparin-affinity chromatography. The biological activity of the purified 43-kDa homodimer was demonstrated by high-affinity binding to VEGF receptors, and by the induction of DNA synthesis in vascular endothelial cells. A positive angiogenic activity in vivo was demonstrated by the day-13 chorioallantoic-membrane assay. The mitogenic potency of VEGF121 for human umbilical vein endothelial cells was very similar compared to VEGF165. These results demonstrate that an angiogenic growth factor whose normal processing requires glycosylation and disulfide-bridge formation can be efficiently expressed in high concentration (up to 5 micrograms/ml) in insects cells.

Amino Acid Sequence↗
PubMed1993

[Aggressive behavior of patients with psychosomatic disorders. A comparative study of patients with ulcerative colitis and diabetes mellitus with the Rosenzweig-P-F Test].

In previous investigations with the Rosenzweig Picture-Frustration Study (PFS) the patients of the psychosomatic sample distinguished themselves by a decreased extrapunitivity and increased intropunitivity in comparison with the "healthy" control persons and by a more distinct intropunitivity compared with neurotic control persons. In this investigation two groups with patients with somatic diseases are compared with the PFS-32 patients with ulcerative colitis (psychosomatic group) and 30 patients with diabetes mellitus (somatic control group). The patients with colitis attain significantly higher scores on the dimension intropunitivity, i.e. they turn aggressive impulses against self to a greater extent. The result is in accordance with the assumption of an aggressive inhibition of patients with ulcerative colitis often described in the literature.

Adaptation, Psychological↗
PubMed1993

[Physical activity, nutritional counseling and psychological guidance in treatment of obese children. A controlled follow-up study over six months].

BACKGROUND: Only multi-faceted treatment programs may influence childhood obesity. METHODS: Thus our program consists of physical exercise, dietary counseling (but no caloric reduction) and psychological support. 17 obese children, aged 7 to 12 years, participated in this combined out-patient therapy program. The children were compared to a control group of 17 obese children who only received an initial dietary instruction. RESULTS: After 6 months program children showed a very significant decrease of percentage overweight (-11.5%), whereas control children showed an increase of +2.8%. In contrast to control children program children improved their physical performance rather significantly. Both groups improved in body-esteem, whereas only program children showed a better self-esteem. Positive trends in lipid metabolism did not reach level of significance. CONCLUSIONS: We conclude that a combined program of exercise and diet improves short-term results in the treatment of childhood obesity, even without insisting on caloric reduction.

Behavior Therapy↗
PubMed1992

Heterogeneity of three electrophoretically distinct Go alpha-subunits in mammalian brain.

So far three splice variants of the alpha o-gene coding for two alpha o proteins have been identified by molecular cloning, and the corresponding proteins purified. In the present study subtype-specific peptide antibodies revealed the existence of an electrophoretically distinct third form of alpha o in mammalian brain membranes which migrates more slowly on SDS-PAGE and shows a more acidic pI value than the other alpha o-subunits. Each of the three alpha o-subunits is detected as two isoforms when enriched from brain membranes. Rodent alpha o-subunits differ from non-rodent species in their electrophoretic mobilities. The results suggest that (i) there may exist a novel alpha o-subunit which reacts with an alpha o1-subunit-specific antibody, (ii) each alpha o-subunit may exist in more than one co- or posttranslationally modified isoform in brain membranes, and (iii) differences between alpha o-subunits from different species exist which are detectable by gel electrophoretic methods.

Amino Acid Sequence↗
PubMed1991

The stepwise degradation of a glycosylated aldose. A potential method for sequencing branched oligosaccharides.

2,3,6,2',3',4',6'-Hepta-O-methylmaltose diethyl dithioacetal (2), obtained under mild conditions from N-methyl-hepta-O-methyl-N-p-nitrophenyl-beta-maltosylamine (1), was converted into the disulfone 4. Storage of a solution of 4 in aqueous tetrahydrofuran gave 1,2-dideoxy-1,1-bis(ethylsulfonyl)hepta-O-methylmalt-1-en itol (5). Fragmentation of 5 with aqueous ammonia released 2,3,4,6-tetra-O-methyl-D-glucose. The degradation procedure has potential for the sequencing of branched oligosaccharides.

Carbohydrate Sequence↗
PubMed1991

Therapy outcome of two treatment models for bulimia nervosa: preliminary results of a controlled study.

Beyond the reduction of the core symptoms, different modification patterns are expected due to differing emphases in two alternative types of treatment for bulimia nervosa: inpatient analytic and outpatient systemic therapy. The initial results of a study with a waiting-list control group are reported. Eating disorders of the bulimic women definitely improved in both therapy groups, the results for the inpatient group (n = 27) indicate a basic change in the attitude towards eating.

Adult↗
PubMed1991

Everyday addictive behavior of bulimic women.

The comorbidity of bulimia nervosa and other forms of drug use or dependence is frequently reported, but statistical comparison as well as reference to a theoretical background is rare to be found. In order to assess the associated dependencies of bulimia, 105 bulimic women participated in a structured interview with a focus on their everyday drug use. Our results suggest that there is little evidence of other forms of related addicting drugs among bulimics. A possible theoretical background which explains the resulting pattern of their taking of stimulants and drugs is the common belief about desired effects of the consumption of these substances.

Adolescent↗
PubMed1990

[A concentration measuring error-induced calculation error in determining uric acid clearance in dialysis: a simulation study].

Three calculation methods to estimation of the total clearance of dialysis patients are evaluated. The basis of calculation are concentration values of urea in blood versus dialysate. The variation coefficient of the measurement errors is 5 percent. The error formation in three estimation formulas is examined. The formula [formula: see text] is most suitable, if as evaluation criteria the variation coefficient of the estimation error are used. The parameters like dialysate flow, blood flow, ultrafiltration rate and dialysate volume are of relative value.

Blood Flow Velocity↗
PubMed1990

[Computed tomographic-guided fine-needle biopsy of the pancreas for histology determination].

Computed tomography-guided fine-needle biopsies of the pancreas were performed in 54 patients. In 46/54 biopsies, the material obtained permitted a histological diagnosis despite the small external needle diameter of only 0.95 mm. The only complication was due to laceration of an artery of the transverse mesocolon. The prevalence of malignant tumors was 67%. Adenocarcinoma was diagnosed in more than 80% of all biopsies; rare findings were malignant carcinoid or centroblastic lymphoma. Benign tumors included mucinous adenocystoma and serous microcystic adenoma. Within the group of patients where pathologic-anatomic evaluation was possible, the sensitivity of fine-needle biopsy for malignant tumors was 87% and the specificity 100%. A positive needle biopsy diagnosis for a malignant tumor reduces the number of exploratory laparotomies needed and is helpful in the planning of surgery.

Adenocarcinoma, Mucinous↗
PubMed1990

[Bulimia with and without a history of anorexia--variants or entities?].

A group of bulimic women (n = 70) with (n = 23) and without (n = 47) a history of anorexia nervosa has been assessed concerning symptomatology, complaints and personality factors. Demographic variables were controlled for bias. As we had supposed, there was no difference for most of the variables analyzed. Some of the residual differences could be due to item formulation ("Oral Control"; EAT) or express the slight persisting underweight ("weight", "underweight") of the former anorexics. Nevertheless, this group unexpectedly seems to feel not equally disturbed concerning "Ineffectivity" (EDI), "Anancasm" (ANIS) and some personality factors ("Social Orientation", "Aggression", "Sorrows concerning health", "Neuroticism"; FPI-R). The similarity is even more impressive if the scores are seen in relation to population norms (Graph.). On demographic variables except weight both groups seem to be strikingly similar. The relative weight (or "underweight"), which was considered as a possible covariate, did not contribute significantly to most of the dimensions, so that the actual body shape cannot be viewed as significantly influencing personality and eating related factors. From the data analyzed we conclude that there are no reasons to consider bulimia with and without a former anorexia as distinct disorders.

Adult↗