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Biomedical subjects

B Herrmann

Publications and source records attributed to B Herrmann.

At least 109 records · Page 6Linked to original sources
PubMed1984

Molecular clones of the mouse t complex derived from microdissected metaphase chromosomes.

Fragments of the proximal half of mouse chromosome 17 including the t-complex region were microdissected from metaphase spreads. DNA was isolated from a pool of such fragments, and was cloned on microscale. Individual clones were used to probe genomic digests of DNA from a pair of Chinese hamster cell lines with or without mouse chromosome 17, and livers of congenic inbred lines of mice carrying wild-type and/or t-haplotype forms of chromosome 17. The data obtained indicate that 95% of the low copy number microclone inserts recognize DNA sequences present on mouse chromosome 17. It has been possible to use one-third of these clones to identify restriction-fragment-length polymorphisms between wild-type and t-haplotype DNA on a congenic background. These results demonstrate that these clones have been derived from the t-complex or regions closely linked to it. Clones of this type should provide starting points for a molecular analysis of this region of the mouse genome.

Animals↗
PubMed1983

[Tetracycline-like fluorescence in buried human skeleton parts].

Some ubiquitous soil-living micro-organisms are producing tetracycline-like fluorescences in skeletal remains. For this reason, the possibility of using tetracycline fluorescences in dating or allocating displaced skeletal parts is severely limited.

Bone and Bones↗
PubMed1983

[Importance of the modern hospital diet in medical practice].

Hospital diet is an essential part of modern therapy in all medical departments. It comprises both the so-called normal diets which are prepared according to modern nutritional knowledge, dietetic foods and the various forms of artificial nutrition (nutrient-defined and chemically defined diets, parenteral and combined nutrition, i.e. enteral and parenteral nutrition). The integration of nutritional therapeutic problems into the treatment of patients requires optimal scientific and practical forms of organisation of nutrition in the clinic. For that purpose, cooperation of clinical, technical, economic and pedagogic divisions is of prime importance. The organisation of patient nutrition in the St. Georg District Hospital in Leipzig is described as a feasible model.

Deficiency Diseases↗
PubMed1983

[Possibilities of metric sex determination from the pars petrosa ossis temporalis].

Based on a sample of petrous bones of known sex (47 males and 47 females) discriminant functions were worked out for sexing both uncremated and cremated petrous bones. Classification values were certain to range from 70.2% to 76.6% for uncremated petrosals and from 67.0% to 73.4% for cremated ones. The results do not support the more promising values suggested by Wahl (1981).

Adult↗
PubMed1983

Role of genes 4 and 6 for the expression of some biological properties of influenza virus A/PR/8/34.

Genetic composition and biological properties of influenza virus recombinants A/PR/8/34 (H1N1) and A/Greifswald/6/74 (H3N2) were analysed. The haemagglutinin (HA) gene of the strain A/PR/8/34 was shown an important part of the gene complex determining the virulence for mice, the yield of HA and the plaque forming capacity. The exchange of the HA gene with that of an another strain led to a drastic reduction of these properties. On the other hand, the introduction of the HA gene of A/PR/8/34 strain into the genome of an another strain, did not render the latter virulent for mice. The production of H3 HA proceeded at an about 5-fold higher extent when the H3-gene was cooperating with the genes of A/PR/8/34 in contrast to the genes of A/Greifswald/6/74.

Animals↗
PubMed1982

[Long-term decomposition of bones. 1. Mineral phase].

Based on the alteration of microstructure and gross features caused by long term fate of bony substance a model of decomposition of bone mineral is outlined. Destruction of the corps by microorganisms leads on the whole to an acid milieu. Hence hydroxyapatite is turned into brushite, which is more soluble in acid media. As this mineral is formed in a space consuming way, the expanding brushite-masses support mechanical destruction of bony substance by cracking the lamellary systems. Both mechanical stress by formation of brushite and transformation of hydroxyapatite to this mineral compound are main features for dead bone decomposition.

Bone and Bones↗
PubMed1980

Selective guanosine phosphate deficiency in hepatoma cells induced by inhibitors of IMP dehydrogenase.

Inhibition of IMP dehydrogenase in AS-30D hepatoma cells in suspension culture resulted in a pronounced and selective reduction of guanine nucleotide pools. Total acid-soluble guanine nucleotides decreased to 40% and the content of GTP and GDP dropped to about 20% of control within 4 h when mycophenolate or ribavirin were used as the inhibitors. Induction of GTP deficiency was associated with a 50% rise in UTP and other uracil nucleotides. Guanosine rapidly reversed both the reduction of guanine nucleotide pools and the elevation of cellular UTP contents. Enzymatic nucleotide analyses in cell and tissue extracts after treatment with ribavirin indicated that ribavirin 5'-triphosphate was an effective substrate for yeast hexokinase, yeast phosphoglycerate kinase, and nucleosidediphosphate kinase from yeast or bovine liver. These results were confirmed in detail by the use of synthetic ribavirin 5'-triphosphate and 5'-diphosphate. The latter nucleotide analog was also a substrate of pyruvate kinase from muscle. Mycophenolate-induced GTP deficiency was associated with an arrest of hepatoma cell growth in suspension culture. Ribavirin, at an equimolar concentration, was much less effective in this respect. None of the two inhibitors had a detectable effect, however, in vivo when guanine or uracil nucleotides were assayed in liver. This indicated that an inhibition of de novo guanylate synthesis in vivo can be compensated by salvage pathway synthesis.

Animals↗
PubMed1978

[The use of childbirth alterations of the pubic bone for identification (author's transl)].

This paper discusses whether parturition or the number of parturition events can be determined from certain gross alterations, which are caused by pregnancy and parturition, found around the symphysis area of the pubic bone. The study is based on the analysis of 49 pairs of pubic bones, for which the number of parturitions and/or abortions were known. No criteria were found to be useful for a clear forensic diagnosis. However, this investigation did show a certain probability of correlation between the extension of grooves on the posterior pubic cortex and the number of parturitions. Examples of remarkable misleading conclusions in special cases are given.

Adult↗
PubMed1978

[Production of influenza virus recombinations].

Described in this paper is the preparation of influenza virus recombination partners, H3N2 and H3N1, by poly-infection of primary embryonic fowl cells with influenza virus A/Greifswald/6/74 (H3N2) or with A/Greifswald/1/76 (H3N2) and A/PR/8/34 (H0N1). Purification was based on four consecutive plaque isolations. The productivity of all recombination partners isolated in the embryonated fowl egg was higher than that of parent strain, H3N2. A recombination partner of H0N2 was obtained from poly-infection of isolated chorio-allantois membranes (CAM) with inactivated influenza virus A/PR/8/34 (H0N1) and A/Greifswald/6/74 (H3N2), the whole process being exposed to the action of serum anti-H3N1. Purification was undertaken via plaque passages, as well. The recombination partners could be characterised by determination of haemagglutination titres in the embryonated fowl egg and by reference to plaque diameters.

Allantois↗