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Biomedical subjects

B Helpap

Publications and source records attributed to B Helpap.

At least 163 records · Page 9Linked to original sources
PubMed1977

[Comparison between frozen section histology and touch cytology of the breast].

Tissue biopsies of the breast with and without carcinomas were examined simultaneously by intraoperative histology and imprint cytology. In 95 per cent of the cases there was a good correlation between the histologic and cytologic diagnoses. The reliability of imprint cytology was tested in some complicated cases such as proliferating fibroadenomas, pseudoinvasive adenosis and carcinomas. In 20,2 per cent of the cases with carcinoma, the tumor cells showed peculiar intracytoplasmic inclusions, whereas such inclusions were found only in 0,43 per cent of the biopsies of mammas without carcinoma. Their morphological variations and the histochemical pattern are discussed. The results have demonstrated that simultaneous cytologic examination of unfixed mamma biopsies can be a good screening method and that the intracytoplasmic inclusions may be an especially helpful histopathologic feature in the diagnosis of breast cancer.

Biopsy↗
PubMed1977

The wound healing of the liver after thermonecrosis. Autoradiographical investigations with 3H-thymidine on rats.

Histological and autoradiographical studies were performed on the liver of rats after local thermonecrosis. The results were compared with those after local cryonecrosis. After thermocoagulation (740 degrees C/4 sec) each animal received an i.p. injection of 3H-TdR (2.5 micronC/1 g body weight). The survival times ranged from 12 hours to 30 days. In the HE stained histological slides the morphological changes and the percentages of the cells in the granulation tissue and in the stripping film autoradiograms the percentages of radioactively labeled fibroblasts and hepatocytes were determined. After 12--24 hours the thermonecrosis is complete with leucocytic demarcations. Five days later widespread granulation tissue develops in the marginal zone of the necrosis. 20 to 30 days after thermocoagulation small necrotic parts are still seen in the center sorrounded by a fibrous scar. Autoradiographically the highest percentages of labeled epithelial and mesenchymal cells are observed between the second and the third postoperative day. Four weeks after thermonecrosis there are still increased cellular proliferative activities. Therefore, it can be stated that the inflammatory resorptive woundhealing processes following thermonecrosis in the liver are maintained much longer than after other physical injuries especially after cryonecrosis.

Animals↗
PubMed1977

[Morphological findings on the brain of rats after local freezing (author's transl)].

In continuation of our investigations of the wound healing process after cryonecrosis in the liver, kidney, spleen and stomach, the wound healing in the brain was studied under the same condition. Through a trepanation of about 3 mm diameter, the cortex of the parietal region of the cerebrum was frozen by a cryoprobe applied directly through the intact dura mater (-196degreesC/30s). The animals were killed at definite time intervals between 12 h and 21 days after freezing. Twelve hours after freezing, the necrosis of the brain cortex is complete. It extends wedgeshaped into the subcortical white matter. The central parts of the necrosis become colliquative and are demarcted by leucocytes. Two days after freezing, the necrotic area is almost totally invaded by leucocytes. Three days p.op. the brain wound is infiltrated by microglial cells, later on the mesenchymal cell proliferation extends to the meninges. There is new capillary formation and partially a wall like proliferation of the perivascular connective tissue cells. Ten days and later, an uni- or multiloculated pseudocyst develops, which can be considered as the final stage of the reparative wound healing of the cryonecrosis. Similar as in the other investigated organs (liver, kidneys, spleen) these investigations underline the rapid wound healing of the cryonecrosis in the brain. The advantages of cryosurgical manipulations with the well known good wound healing process of other organs, justifies therefore the short term tissue freezing on the cerebrum.

Animals↗
PubMed1977

[Primary intratympanic meningioma (author's transl)].

A primary intratympanic meningioma is reported, which was histologically verified. 1 1/2 and 2 1/2 years after surgical removal, there were no signs of recurrence, neither clinically nor histologically. Sites of origin of the primary intratympanic meningiomas are discussed. Symptoms, diagnostics, differential diagnosis, therapy and prognosis are summarized in relation to nine cases of the literature.

Ear Neoplasms↗
PubMed1977

Malignant papillomatosis of the intrahepatic bile ducts.

A case of intrahepatic bile duct papillomatosis is reported which recurred several times and finally progressed into a mucin-secreting papillary adenocarcinoma. The paillomatosis predominantly involved the ductal system of the left hypoplastic hepatic lobe. The treatment consisted of a left partial hepatectomy. Hereby, a four-year survival was reached. While the prognosis of solitary papillomas in the distal choledochus and in the gallbladder is considered as good, the prognosis of the intra- and extrahepatic bile duct papillomatosis appears unfavorable. The experience in this case, as well as in the literature, shows that these papillomatoses have to be regarded as low-grade carcinomas.

Adult↗
PubMed1977

Cell-kinetical analyses of squamous cell carcinomas of the oral region and the effect of a combined therapy of 5 fluorouracil and irradiation. A contribution to the discussion about tumorcell-synchronization.

Out of 180 incubations of human biopsies 33 were from patients with malignant tumors of the oral region. In 9 cases it was possible to analyse the cell cycle of the tumor cells with the double labeling in vitro method (3H and 14C-thymidine). Histologically the tumors were keratinizing squamous cell carcinomas with high labeling indices. The generation time of the tumor cells ranged from 20 to 130 hours. The length of the DNA synthesis phase varied between 7.3 and 17.9 hours. With the knowledge of the cell-kinetic data the patients received an infusion of 5 Fluorouracil throughout the length or parts of the calculated G 1 phase. At the end of the 5 FU infusion there was a constant two-hour pause and after the duration of the calculated DNA synthesis phase the tumors were irradiated with 150 rd per session. The treatment was repeated until a total dose of 6000 rd was achieved. Under the combined therapy of 5 FU and irradiation the inoperable keratinizing highly differentiated squamous cell carcinomas regressed extraordinarily fast. The patients were free of pain after a very short time. No vital tumor tissue was visible in control biopsies. Some patients remained without any tumor recurrence now more than 2 years after onset of therapy. In the discussion it is stressed that the reason for the successful treatment of highly differentiated squamous cell carcinomas with 5 Fluorouracil and subsequent irradiation is not easily explained, because recent experimental findings and impulsecytophotometrical studies on human biopsy material suggest that both the synergistic action of thecytostatic drug and the irradiation as well as an altered recruitment of the tumor cells are responsible for the clinical success rather than a synchronisation effect.

Adult↗
PubMed1976

Wound healing of the brain of rats after cryonecrosis. Autoradiographic investigations with 3H-thymidine.

Histologic and autoradiographic studies were performed to investigate the cellular reactions during wound healing of the brain of rats after cryonecrosis. A parietal lobe was frozen for 30 s with a cryoprobe at a temperature of -196 degrees C. The survival time ranged between 12 h and 21 days. One h before killing, tritiated thymidine was injected intraperitoneally. Stripping film autoradiograms of the brain sections were made in the usual manner. A typical cryonecrosis develops 12 h after local freezing surrounded by an edematous tissue layer with activated mesenchymal and neurologlial cells. After 10 days a pseudocyst develops surrounded by highly cellular glial tissue. The pseudocyst is still recognizable 3 weeks later but without any remarkable cellular reaction. Autoradiographically the labeling indices of the fibroblasts, leptomeningeal,and endothelial cells in the periphery of the necrosis and the labeling indices of neuroglial, perivascular connective tissue, and microglial cells in the perinecrotic zone increase after 12 h and have their maximum between the 2nd and 3rd postoperative day. In the nonfrozen contralateral cerebral hemispheres, the labeling indices of neuroglial and mesenchymal cells show small peaks in the first 3 postoperative days,also. This can be explained by accompanying edema. There are no remarkable differences between frozen and nonfrozen parts of the brain 3 weeks after local freezing. The results underline the rapid repair of cryonecrosis.

Animals↗
PubMed1976

[Intracytoplasmic inclusions within cells of the breast and their diagnostic significance (author's transl)].

There were 700 tissue biopsies of the breast with and without carcinomas examined simultaneously by intraoperative histology and imprint cytology. In 20.2% of the cases with carcinoma the tumor cells showed peculiar intracytoplasmic inclusions, whereas in only 0.43% of the biopsies of the mamma without carcinoma such inclusions were to be found. Their morphologic variation and the histochemical pattern are discussed. It is pointed out that these intracytoplasmic inclusions may be a helpful histopathologic feature in the diagnosis of breast cancer.

Breast↗
PubMed1976

[Pulmonary rhabdomyosarcoma (author's transl)].

A 55-years old patient is reported who became ill with recurrent attacks of coughing and haemoptysis some 3 weeks before death. An extensive mediastinal tumor was demonstrated radiologically. Pathoanatomical examination showed a pleomorphic rhabdomyosarcoma of the lower left pulmonary lobe with metastases in the right lung and regional lymph nodes.

Autopsy↗
PubMed1976

Tissue culture, electron microscopic and enzyme histochemical investigations of extraadrenal paragangliomas.

Light and electromicroscopical as well as histochemical investigations were performed on three cases of extraadrenal paragangliomas. They were localized in the carotid body, tympanicum and cauda equina region. Tissue of two cases was cultivated in vitro in nutrient medium TCM 199. The tumours were classified as paragangliomas of the paraganglionic type with typical cell clusters, of the adenomatous and angiomatous type. The enzyme histochemistry showed a very high dehydrogenase activity. Ultrastructurally numerous typical osmiophilic granules could be observed in the cytoplasm of the tumour cells. In tissue culture only a minimal cellular proliferative activity could be detected. The few proliferating cell colonies showed mostly characteristics of epithelial tissue and sometimes a similar behaviour to cells of a ganglioneuroblastoma. The minimal proliferative activity in vitro is in good agreement with the proliferative behaviour of the extraadrenal paragangliomas in vivo.

Carboxylic Ester Hydrolases↗
PubMed1976

[Report on 2 cases of malignant hyperthermia with different clinical courses].

Two cases of malignant hyperthermia with different clinical courses are reported. The patients showed the classical signs of malignant hyperthermia consisting of tachycardia, tachypnoea, ocasional peripheral cyanosis, high body temperature as well as characteristic rise in serum enzymes. In one of the patients the symptoms were recognized early during the operation. The immediate commencement of therapy with ice-cooled. Ringer-Lactate-Solution, Procainmedication, Corticoids as well as physical body cooling favourably influenced the clinical course and the patient survived. In both cases the patients underwent succinylcholine and halothane anaesthesia, but the symptoms of the second patient appeared after the reduction of anaesthesia. In spite of vigorous therapy the hyperpyrexia resulted in heart arrest and death. Morphologically, both patients showed signs of preexistent myopathy with volumetric alterations of the muscle fibres, centralisation of the nuclei and acute muscle fibre necrosis. On the basis of the observed variable course, the various symptom complexes reported in the literature to data are reviewed. A detailed discussion of the "carrier problem" and the available treatment possibilities is also made. Realising that malignant hyperthermia is an inheritable disease, prophylactic measures such as, f.i. the issue of medical certificates to the patient and his relatives are suggested.

Adult↗
PubMed1975

[Comparative studies of simultaneous intraoperative frozen sections and imprint cytology of the mamma (author's transl)].

250 tissue biopsies of the mamma were examined simultaneously by intraoperative histology (frozen sections) and imprint cytology. In 95% of the cases there was a good agreement between the histologic and cytologic diagnoses. The reliability of the imprint cytology was tested in some complicated cases such as proliferating fibroadenomas, sclerosing adenosis and carcinomas and the significance of the imprint cytology for the diagnosis of difficult cases was pointed out in detail. The results have demonstrated, that such a cytologic examination can not be a substitute for intraoperative frozen sections - but the simultaneous cytologic examination of unfixed mamma biopsies can be a good searching method and can be helpful in the intraoperative diagnostic.

Adenocarcinoma, Mucinous↗
PubMed1975

Histological and autoradiographical findings in the immunologically stimulated spleen.

In order to estimate the role of a possible immunological coeffect on the splenic cellular proliferation during wound healing after mechanical or thermal lesions of internal organs, light microscopical and autoradiographical investigations with tritiated thymidine were performed on spleens of non-germfree rats, immunized by a single injection of sheep erythrocytes. Two days after an initial dissociation of preexisting germinal centers in the spleen a steep rise of the percentages of labeled cells in newly formed germinal centers occurs with a maximum on the fifth day. In the marginal zone an increased cell proliferation (mostly large lymphoid blasts) starts at 12 hours. In the lymphatic mantle zone a marked increase of labeled basophilic blasts (immunoblasts) can be observed with a maximum on the second day. The values are higher in the perifollicular B cell region than in the periarteriolar T cell region. In the red pulp of the spleen the highest percentages of labeled cells occur five days after the antigen injection. These findings in the spleen after a strong antigenic stimulation are characteristic for an immunological reaction of anamnestic type. In comparison with investigations of the spleen after cryolesions in internal organs such studies may be particularly helpful in judging the proportion of an unknown immunological reaction in the spleen after such an operation, which by tissue necroses and cellular destruction may have caused denaturation of self components.

Animals↗