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Biomedical subjects

B Hale

Publications and source records attributed to B Hale.

At least 19 recordsLinked to original sources

Culpability and blame after pregnancy loss.

The problem of feeling guilty about a pregnancy loss is suggested to be primarily a moral matter and not a medical or psychological one. Two standard approaches to women who blame themselves for a loss are first introduced, characterised as either psychologistic or deterministic. Both these approaches are shown to underdetermine the autonomy of the mother by depending on the notion that the mother is not culpable for the loss if she "could not have acted otherwise". The inability to act otherwise is explained as not being as strong a determinant of culpability as it may seem at first. Instead, people's culpability for a bad turn of events implies strongly that they have acted for the wrong reasons, which is probably not true in the case of women who have experienced a loss of pregnancy. The practical conclusion of this paper is that women who feel a sense of guilt in the wake of their loss have a good reason to reject both the psychologistic and the deterministic approaches to their guilt-that they are justified in feeling upset about what has gone wrong, even responsible for the life of the child, but are not culpable for the unfortunate turn of events.

Abortion, Spontaneous↗

Effect of incremental exercise on initiation and movement times in a choice response, whole body psychomotor task.

OBJECTIVES: To examine how exercise at moderate and maximal intensities affects performance on a choice response time, whole body psychomotor task. METHODS: Subjects (n = 12) were tested on a three-choice response time task, after rest and after exercise at 70% and 100% maximum power output (W*max). The dependent variables were time to begin forward momentum (initiation time) and time to complete the movement (movement time). Stride time for the first and second strides and number of strides to cover first 1.1 m were also measured. Blood lactate concentrations and heart rate were recorded before and after completion of each psychomotor test. The subjects subjectively assessed the amount of effort that they used to complete the task. RESULTS: Repeated measures analysis of variance showed a significant effect for initiation (F2,22 = 11.47, p < 0.001) and movement times (F2,22 = 14.61, p < 0.001). Post hoc least significant difference (LSD) tests showed that initiation time after exercise at 70% W*max was significantly faster than that in the other two conditions. Speed of initiation after rest was significantly quicker than that after exercise at W*max. For movement time, LSD tests showed that time after maximal exercise was significantly slower than that in the other two conditions. Stride time for the second stride showed a significant effect (F2,22 = 6.20, p < 0.01). LSD tests found that time after exercise at W*max was significantly slower in the other two conditions. Stepwise multiple regression analyses found that the increment of change, from rest, of lactate concentrations could significantly predict the increment of change in initiation (R2 = 0.40) and movement (R2 = 0.50) times. CONCLUSIONS: Exercise affects a whole body task differently from purely cognitive tasks. Central factors are probably more important than peripheral factors.

Adult↗

Exercise-dependence in bodybuilders: antecedents and reliability of measurement.

AIM: The purposes of this study were: a) to examine social and psychological antecedents of bodybuilding dependence (life satisfaction, socio-economic status, marital status and parental status) and b) to examine the test-retest reliability of the Bodybuilding Dependence Scale (BDS). METHODS: One hundred and eighty-one male bodybuilders agreed to participate in the study. Nineteen of these failed to complete the questionnaire on the second occasion, resulting in a sample size of 162. Each participant completed the BDS on two occasions 3 weeks apart, together with the Satisfaction with Life Scale (SWLS) and items relating to occupation, marital status and parental status. RESULTS: Pearson correlations revealed high test-retest reliability for all three subscales (r=0.94, 0.96 and 0.94 for social-dependence, training-dependence and mastery-dependence, respectively). Pearson correlations also revealed significant negative correlations between all three BDS subscales and SWLS scores (r=-0.66 in all cases). A MANOVA revealed that working class participants scored significantly higher on all three BDS subscales than intermediate class participants who, in turn, scored significantly higher than professional class participants. Participants who were not currently involved in a romantic relationship scored significantly higher on all BDS subscales than those who were romantically involved. Parents scored significantly higher than non-parents on social and mastery-dependence, but not on training-dependence. CONCLUSION: These results show acceptable test-retest reliability for all three BDS subscales. They also demonstrate that life satisfaction, socio-economic status, marital status and parental status can successfully predict BDS scores.

Adult↗

The effect of pH, time and dietary source of cadmium on the bioaccessibility and adsorption of cadmium to/from lettuce (Lactuca sativa L. cv. Ostinata).

This study evaluated the influence of three variables in the effectiveness of an in vitro digestion protocol used to determine bioaccessibility of cadmium from the diet. The percentage of solubilized metal was measured in relation to digestion time, pH of each digestion phase and the dietary source of the metal in the diet. Because it would be convenient to add the metal to the diet before digestion instead of growing contaminated vegetables, the importance of metal incorporation in the plant in comparison to amendment through foliar spraying was also studied. From our results we conclude that the dietary source of metal in the protocols tested doesn't seem to be a significant factor when comparing the lettuce sprayed with cadmium with the lettuce that had cadmium incorporated in it, although the difference was barely significant (P=0.057). Time affects the digestion in different ways depending on the dietary source of cadmium. pH is a relevant factor in both intestinal and gastric phases and should be taken into consideration when analyzing the results from in vitro digestions. Since the intestinal phase in our experiments decreased the amount of cadmium solubilized during the digestion, we investigated the effect of pH on the adsorption of this metal to lettuce and found that there is an increased binding of cadmium at pH values above 3. Therefore we suggest that part of the reduction in bioaccessibility following intestinal digestion could be explained by an increase in adsorption of metal to the plant material at higher pH values.

Adsorption↗

Validity and factor structure of the bodybuilding dependence scale.

OBJECTIVES: To investigate the factor structure, validity, and reliability of the bodybuilding dependence scale and to investigate differences in bodybuilding dependence between men and women and competitive and non-competitive bodybuilders. METHODS: Seventy two male competitive bodybuilders, 63 female competitive bodybuilders, 87 male non-competitive bodybuilders, and 63 non-competitive female bodybuilders completed the bodybuilding dependence scale (BDS), the exercise dependence questionnaire (EDQ), and the muscle dysmorphia inventory (MDI). RESULTS: Confirmatory factor analysis of the BDS supported a three factor model of bodybuilding dependence, consisting of social dependence, training dependence, and mastery dependence (Q = 3.16, CFI = 0.98, SRMR = 0.04). Internal reliability of all three subscales was high (Cronbach's alpha = 0.92, 0.92, and 0.93 respectively). Significant (p<0.001) and moderate correlations were found between all BDS and MDI subscales, and between five of the eight EDQ subscales. A multivariate analysis of covariance, with univariate F tests and Tukey HSD tests, revealed that both male and female competitive bodybuilders scored significantly (p<0.05) higher on all three BDS subscales than the male and female non-competitive bodybuilders. However, there were no significant sex differences on any of the BDS subscales (p>0.05). CONCLUSION: The three factor BDS appears to be a reliable and valid measure of bodybuilding dependence. Symptoms of bodybuilding dependence are more prevalent in competitive bodybuilders than non-competitive ones, but there are no significant sex differences in bodybuilding dependence.

Adult↗

Exercise dependence, social physique anxiety, and social support in experienced and inexperienced bodybuilders and weightlifters.

OBJECTIVES: To investigate psychological correlates of exercise dependence in experienced and inexperienced bodybuilders and weightlifters. Secondary objectives included measuring social physique anxiety, bodybuilding identity, and social support among bodybuilders and weightlifters. METHODS: Thirty five experienced bodybuilders, 31 inexperienced bodybuilders, and 23 weightlifters completed the bodybuilding dependence scale, a bodybuilding version of the athletic identity measurement scale, the social physique anxiety scale, and an adapted version of the social support survey-clinical form. RESULTS: A between subjects multivariate analysis of variance was calculated on the scores of the three groups of lifters for the four questionnaires. Univariate F tests and follow up tests indicated that experienced bodybuilders scored significantly higher than inexperienced bodybuilders and weightlifters on bodybuilding dependence (p<0.001), social identity and exclusivity subscales of bodybuilding identity (p<0.001), and social support scales (p<.001), and significantly lower on social physique anxiety (p<0.001). CONCLUSION: Experienced bodybuilders exhibit more exercise dependence, show greater social support behaviour, and experience less social physique anxiety than inexperienced bodybuilders and weightlifters.

Adult↗

Urban-rural hospital affiliations: assessing control, fit, and stakeholder issues strategically.

Urban-rural hospital affiliations are an outgrowth of both the external pressures on rural hospitals to survive and the need for urban hospitals to maintain or increase their share of the tertiary referral market. This article discusses the significant role of stakeholders in these affiliations, develops a fourfold typology of urban-rural hospital affiliations based on the notions of organizational control and fit, suggests four generic strategies for forming affiliations, and analyzes four actual cases of affiliation.

Conflict, Psychological↗

Inactivation of intracellular copper-zinc superoxide dismutase by copper chelating agents without glutathione depletion and methemoglobin formation.

The copper chelator N,N'-diethyldithiocarbamate (DDC), is often used to inactivate intracellular copper-zinc superoxide dismutase in erythrocytes. However, in studies with red cells we found that the compound also reacted with oxyhemoglobin to produce oxygen radicals in addition to generating lipid peroxidation products, oxidized N,N'-diethyldithiocarbamate, methemoglobin, and sulfhemoglobin. Moreover, intracellular glutathione was depleted and vital cellular enzymes were susceptible to inactivation. We, and others, have confirmed these findings in nonerythrocytic cell lines. Thus, cells exposed to DDC are severely damaged before studies on the effects of added putative superoxide producing compounds can be performed with them. In this report, we have systematically investigated other copper chelators for their ability to inactivate intracellular copper-zinc superoxide dismutase without producing the deleterious effects mentioned above. Catechol, triethylenetetramine, and tetraethylenepentamine were found to be such agents when erythrocytes were dialyzed in the cold against dilute solutions of these chelators. In addition, with a myeloid leukemic cell line (HL-60), triethylenetetramine inhibited SOD without causing significant GSH oxidation. Examination of the affinity constants of chelators active against purified copper-zinc superoxide dismutase indicated that an affinity binding constant (log K1) between 12.6 and 13.8 was required for the chelator to successfully remove copper from the enzyme.

Cell Line↗

Methylene blue competes with paraquat for reduction by flavo-enzymes resulting in decreased superoxide production in the presence of heme proteins.

Methylene blue competes 100 to 600 times more effectively than paraquat for reduction by three different flavo-containing enzymes; xanthine oxidase, NADH cytochrome c reductase, and NADPH cytochrome c reductase. Paraquat and methylene blue both interact with deflavo xanthine oxidase, indicating that neither electron acceptor reacted at the FAD site of the enzyme where molecular oxygen is reduced to superoxide. As the paraquat radical also directly reduced acetylated cytochrome c the hemeprotein could not be utilized for measuring superoxide production in the presence of the herbicide. In the presence of cytochrome c the methylene blue caused a sharp decrease in both paraquat-induced superoxide and hydroxyl radical production.

Cytochrome Reductases↗

Potential of methylene blue to block oxygen radical generation in reperfusion injury.

Methylene blue interacts with xanthine oxidase at the iron-sulfide site in the electron pathway (Scheme I) that is known to serve as an electron-sink connecting the reductive and oxidative sites in both the oxidase and dehydrogenase forms. Thus, shunting of electrons to methylene blue at this site effectively diverts their flow away from the FAD site where molecular oxygen is converted to superoxide radicals. Since the electron affinity constants of xanthine oxidase for electron acceptors are FAD greater than iron/sulfide greater than molybdenum, methylene blue falls between the FAD and iron-sulfide site. Thus, methylene blue effectively inhibits superoxide and hydroxyl radical production while accelerating the conversion of xanthine to uric acid. As methylene blue is already approved for medicinal use in humans and is relatively nontoxic, the drug may have a role in reducing tissue injury associated with reperfusion. We are currently investigating this possibility in animal models.

Animals↗