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Biomedical subjects

B Habanec

Publications and source records attributed to B Habanec.

At least 37 records · Page 2Linked to original sources

[Aneurysmal bone cyst of a rib].

Aneurysmatic bone cyst is rare in ribs; only 37 cases were found in literature up to 1984. In a group of 21 children suffering from aneurysmatic bone cyst only one 13 year-old girl had the lesion in sternal part of the 2nd rib.

Adolescent↗

[Tumors of the thyroid gland in childhood--study of biopsy material].

A group of 29 biopsies from resected thyroid gland was represented by 5 boys and 24 girls of the age under 15 years; the mean age was 12,7 years. There were 7 cases of colloid adenoma, 5 of follicular adenoma, 5 of atypical adenoma and 5 of papillary carcinoma among them. A basedowian goiter was verified in 4 cases, Hashimoto-type thyroiditis was found once, thyroid gland was lacking twice. A shift between clinical and pathomorphological diagnoses was remarkable.

Adenoma↗

[Problems with fine-needle puncture examination of the prostate].

The technology of fine-needle aspiration of prostatic tissue was described and its results discussed in a group of 45 patients. Demonstration of findings included: normal cytology, adenomyomatous hyperplasia with mosaic pattern of epithelial cells, acute purulent inflammation, chronic inflammation with pseudoxanthomatous lipophages , seminal vesicles with atypical non-cohesive epithelial cells, and neoplasia.

Adult↗

[Monstrous epithelium in the seminal vesicles and epididymis].

Monstrous epithelial cells in seminal vesicles, causing diagnostical embarrassment , occur sometimes in fine needle-aspirates of prostate gland. Their frequency was studied in a group of autopsies (6 boys of the age from 6 hours to 10 years, mean age 3,1 years and 34 adult men of the age from 42 to 91 years, mean age 68,4 years). Monstrous cells did not occur in boys but were found in 73,5% of adults. There were similar epithelial monstrosities in epididymis simultaneously. Most of them were found in efferent ductuli (in 76,4%). Big monstrous epithelial cells with conspicuous irregular hyperchromatic nuclei are to be distinguished cautiously from the neoplastic ones. They may be caused by ageing and hormonal influence above all (in analogy to Arias-Stella phenomenon in women).

Adult↗

[Mesothelial reaction to irritation].

Autopsy cases of fibrinous pericarditis were studied in different stages of granulation tissue organization. Flat mesothelium grew higher and hyperplastic, lining the holes in fibrin network and desquamating at the same time. Single rounded mesothelial cells shed into free space and acquired an atypical appearance of big monocytoid cells with nuclear hyperchromasia and sometimes with nucleoli. As the organization developed further, the mesothelial cells produced gland-like structures enveloped in edematous granulation tissue pervaded with inflammatory cells. Mesothelial lesions due to chronic inflammatory irritation were also evidenced by cases of chronic adnexitis. The mesothelial cells showed an ability to simulate invasive growth in abundant fibrous stroma as gland-like, papillary and pseudoinfiltrative structures lined with cuboid, columnar, stratified, mucogenic and metaplastic squamous epithelium. Proliferating mesothelial cells might suggest tumourous character; in some cases they could not be distinguished from mesothelioma or from secondaries of adenocarcinoma.

Epithelium↗

[Cytodiagnosis of pleural exudates with electron microscopy. I. Activated pleural mesothelium].

Detailed examination of 48 pleural exudations showed that a sequence of morphological changes [desquamation, proliferation, activation, degeneration] was a uniform response of mesothelium to various pathological stimuli. Desquamated mesothelial cells grow round and bigger, their nuclei increase, multiply, become irregular and get a coarse chromatin pattern. Their cytoplasm is less often homogeneous, a bit basophil and mostly vacuolized to some extent; fusion of vacuoles can lead to a signet-ring appearance. Provided that highly activated mesothelial cells phagocytize foreign corpuscular material, they cannot be distinguished from the macrophages. Their cytoplasmic organelles multiply progressively. More frequent lysosomes occur sometimes with dense lamellated myelin-like bodies. Glycogen particles increase in number substantially in peripheric cytoplasmic zone. Tonofilaments are very conspicuous, arranged round the nucleus, but disappear during progressive mesothelial activation and degradation. Numerous microvilli are gradually reduced in number, change into voluminous plasmatic blebs, and finally, mesothelial surface grow irregular or smooth. In dubious cases, electron microscopy [transmissive and scanning] gives more precise features of organelles and surface structures in activated mesothelial cells and helps to differentiate them from other exudation cells especially the neoplastic ones.

Adolescent↗

[Cytodiagnosis of pleural exudates with electron microscopy. II. Tumorous exudates of glandular origin].

Detailed examination of 26 pleural exudations by transmissive and scanning electron microscopy gave new cytodiagnostical possibilities. In addition to confirming the light-microscopical criteria of malignancy transmissive electron microscopy characterized the cells of adenocarcinoma by microvilli with common structure, various length, irregular orientation and crossing over or branching. They tend to be a bit widened at their base or top and to cover all the surface of cells. Microvilli in the neighbouring parts of cells are less numerous, irregularly oriented, shortened and interdigitating. Not even here is the surface of carcinoma cells quite smoth in contrast to neighbouring mesotelial cells. Scanning electron-microscopical examination being neither technologically sophisticated nor time consuming enables a scope on cellular surfaces uniformly covered by countless microvilli densely distributed.

Adenocarcinoma↗

[Cytological and electron microscopy diagnosis of neuroblastoma].

Out of three cases of neuroblastoma under observation, the diagnosis could be established from the cytological and electron microscopical examinations of bone marrow in two of them. Slide smears revealed the rosette-shaped pattern characteristic of malignant neuroblastoma, many of which were fitted with dendritic plasmatic processes. Electronography helped to prove intraplasmatically the presence in those cells of dense neurosecretory granules, and--in their plasmatic processes--the presence of microfilaments, microtubules, neurosecretory granules and empty vesicules.

Bone Marrow↗

[The diagnostic significance of cytological and histological examination of secretions, washout materials, catheterization and brush biopsy materials in the diagnosis of malignant bronchopulmonary diseases].

Referring to their group of 325 patients examined by means of all-around endobronchial biopsy material withdrawal the authors present the results of cytological and histological diagnosis. Endobronchially material obtained by direct aspiration of bronchial secretions, by wash-outs, and by catheterization and brush biopsy represents, particularly in peripheral localizations, a significant and often decisive contribution to the objectivization of malignant affections. The authors present and discuss morphological criteria of malignity derived from endobronchially obtained material processed by the method of cytoblocks stressing, at the same time, the possibility of an accurate localization of the pathological process down to the particular segment involved.

Biopsy↗