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Biomedical subjects

B Glimelius

Publications and source records attributed to B Glimelius.

At least 289 records · Page 16Linked to original sources
PubMed1984

Biochemical markers in non-Hodgkin's lymphoma stages III and IV and prognosis: a multivariate analysis.

The prognostic value of different pretreatment laboratory and clinical findings at diagnosis was assessed in a series of 141 patients with generalized non-Hodgkin's lymphoma. Univariate and multivariate survival analysis (Cox's regression model) was performed, using serum analysis of deoxythymidine kinase (S-TK), beta 2-microglobulin, lactic dehydrogenase, alpha 1-acid glycoprotein = orosomucoid (S-alpha 1 AGP), haptoglobin and ferritin. In addition, Hb and the erythrocyte sedimentation rate (ESR) were measured. The clinical variables were age, presence or absence of B-symptoms, histopathology ('low-grade'; 'intermediate grade' and 'high-grade' malignancy) and bone marrow involvement. Of the 8 biochemical markers, all except Hb and the ESR showed a significant relationship to survival. Among the clinical variables, this finding was made for B-symptoms and histopathology. Using a multivariate analysis on all variables, S-TK was found to be the best factor for predicting duration of survival. The only significant additional information was provided by S-alpha 1 AGP. When only the clinical variables were taken into account, it was found that histopathology added significant information to that yielded by B-symptoms in the prediction of the survival time. When the biochemical variables were added to this model, only S-TK was of significant additional prognostic value.

Adolescent↗
PubMed1984

Nuclear DNA record in a malignant rectal carcinoid. A case report.

A colo-rectal resection was performed on a 73-year-old woman with a preoperative diagnosis of rectal carcinoma. Morphological examination of the specimen revealed a rectal carcinoid tumor with regional lymph node and liver metastases. Silver stains demonstrated an argyrophil reaction in the tumor cells, but the argentaffin reaction and immunocytochemistry with monoclonal serotonin antibodies were negative. Imprint cytology of the tumor was examined cytofluorometrically and diploid nuclear DNA values were recorded with only few tetraploid cells (less than 2%.). It is suggested that diploid nuclear DNA values and a low proliferative activity are characteristic of malignant rectal carcinoids.

Aged↗
PubMed1984

Ultrasound in preoperative staging of rectal tumours.

A dynamic ultrasound sector scanner with 5.0 and 7.5 MHz transducers was used for examination of resected bowel specimens with rectal carcinoma. Pathologic structures, i.e. tumour extension into or beyond the bowel wall, and perirectal lymph nodes, were marked. Corresponding parts of the tissue were embedded in paraffin and a detailed comparison between the findings at ultrasound and those in the histopathological sections was made. There was a good accordance between the histopathologic and ultrasonic findings which makes it possible to perform an exact preoperative staging of rectal carcinomas with ultrasound. Twenty patients with primary tumours of the rectum preoperatively underwent ultrasonography with a prototype transrectal probe with the same type of crystals and frequencies. In the 14 cases, however, where ultrasound findings could be compared with the histopathologic findings only six (43%) were correctly staged at ultrasonography. Certain difficulties were encountered in the preoperative examinations with a rigid probe as used in this study.

Humans↗
PubMed1984

Demonstration of an extracellular matrix in multicellular tumor spheroids.

Multicellular spheroids of a human glioma cell line (U-118 MG) and a human thyroid cancer cell line ( HTh -7) were analyzed for the presence of extracellular matrix (ECM) using light microscopy, transmission electron microscopy, and indirect immunofluorescence staining for fibronectin, laminin, and collagen. These studies were supplemented by analyses of glycosaminoglycans using autoradiography or chemical methods after metabolic labeling with [35S]sulfate or [3H]glucosamine in conjunction with various extraction procedures. The results showed that both types of spheroids contained an ECM composed of fibronectin, laminin, collagen, and glycosaminoglycans. The organization of the ECM in the spheroids seemed to be similar to that of tumors in vivo. These findings help justify the use of the spheroid system as an in vitro model for the study of biological phenomena of human tumors in vivo. Furthermore, it is concluded that the formation of an ECM in vitro is not confined to normal cells but can be promoted in transformed cells using appropriate culture conditions.

Cell Line↗
PubMed1984

Local recurrences after surgical treatment for rectal carcinoma.

In a retrospective study of patients treated between 1974 and 1979 with surgery alone for cancer of the rectum or rectosigmoid, a total of 293 patients were evaluated. All original histopathological specimens were re-examined and classified according to the Dukes' staging system and to the Astler - Coller 's modification of this system. Among the 197 (67%) patients operated upon for local cure, 74 patients developed a microscopically proven local recurrence (38%), and in 43 of them this was the only sign of recurrent cancer. There was no clear correlation, however, to the pathological stage. Possible explanations for this finding will be discussed.

Adult↗
PubMed1983

Morphological classification of non-Hodgkin malignant lymphoma. II. Comparison between Rappaport's classification and the Kiel classification.

In a retrospective survey of 334 cases of non-Hodgkin lymphoma (NHL), 250 cases could be classified according to both Rappaport's classification and the Kiel classification. All patients initially in stage I had an excellent prognosis irrespective of histology, whereas patients in stages II, III and IV had a worse prognosis that did not significantly differ between stages. Rappaport's classification could separate stages II-IV into 2 different prognostic groups, one favourable and one unfavourable group. According to the Kiel classification, 3 prognostic groups - favourable, intermediate and unfavourable - were found. The survival rate for favourable Rappaport fell in between favourable-Kiel and intermediate-Kiel. Both subgroups within favourable-Rappaport (nodular lymphomas and diffuse well differentiated lymphomas) could actually be subdivided by the Kiel classification into one group with a more truly favourable behaviour and one with an intermediate behaviour. The extent of follicularity among nodular lymphomas and the presence of plasmacytoid differentiation among the small lymphocytic lymphomas were found to be of prognostic importance. In conclusion, we found that the Kiel classification separated the more indolent lymphomas better than Rappaport's classification. No preference was found concerning the high grade lymphomas.

Actuarial Analysis↗
PubMed1983

Radiation therapy of anal carcinoma.

Between 1978 and 1982, 30 consecutive patients with anal carcinoma were given radiation therapy. Irradiation alone was given to cloacogenic carcinoma and in combination with bleomycin to squamous cell carcinoma. Patients with an early tumour (T1, T2) were treated to 65 Gy (60 Gy + bleomycin) directly, whereas patients with a moderately advanced tumour (T3) were treated to the same radiation dose only if no evidence of residual disease existed after approximately 50 Gy (45 Gy + bleomycin); if a palpable tumour still remained 3 to 4 weeks after the end of the irradiation, surgery was performed. Seven of 7 patients with an early lesion (T1, T2) and 5 of 9 with a tumour in stage T3 treated according to this regime are alive with no evidence of disease and with preserved anal anatomy and function. Only one of 9 patients in stage T3 and T4, also treated with an abdominoperineal resection had viable tumour at surgery and 6 of them are alive without disease; 2 died postoperatively and one developed a recurrence in the urinary bladder. The results indicate that anal carcinomas are sensitive to radiation and often curable by irradiation.

Adult↗
PubMed1982

Radiation therapy of non-Hodgkin's lymphoma stages I and II.

A series of 147 patients, 99 in clinical stage I and 48 in stage II, with localized non-Hodgkin's lymphoma, exclusively given radiation therapy, was retrospectively analysed. Using the Kiel classification, 12 patients (8%) could not be subgrouped with certainty, 63 (43%) were designated as high-graded and 72 (49%) as low-grade malignancies according to the definitions of Gérard-Marchant et coll. (1974). Complete remission was obtained in 93 per cent of the patients in stage I and in 75 per cent in stage II. Most of the failures (68%) were high-grade malignancies. Actuarial and relapse-free survival was determined for stage I and II and stratified by microscopy and extranodal disease. All patients initially in stage I had a good prognosis irrespective of the microscopic type; 60 per cent have remained free from disease. In contrast, only 2 patients (4%, all low-grade) in stage II have remained disease-free. Thus, in stage II irradiation cannot be considered the best treatment, especially in patients with high-grade malignancy, in whom chemotherapy may be curable. In stage I, on the other hand, irradiation seems to be curative in the majority of patients irrespective of microscopic type.

Adolescent↗
PubMed1982

Morphologic classification of non-Hodgkin's lymphoma. I. Retrospective analysis using the Kiel classification.

In a retrospective analysis of 334 cases of non-Hodgkin's lymphoma observed between 1969 and 1978, 250 cases could be classified according to the Kiel classification. Clinicopathologic correlation was analysed for these latter cases. Irrespective of the morphologic appearance, all cases initially in stage I showed an excellent prognosis after radiation therapy alone, whereas the prognosis for stage II was similar to stages III and IV. For stages II-IV, 3 major prognostic groups with significantly differing survival curves were identified. The median survival times were one, 3 and more than 7 years, respectively. The pathologic and clinical significance of the Kiel classification is discussed.

Adult↗
PubMed1982

Preoperative irradiation with high-dose fractionation in adenocarcinoma of the rectum and rectosigmoid.

The incidence of local pelvic recurrence after 'curative' resection for rectal carcinoma is high. Adjuvant irradiation given pre- or postoperatively has led to a decreased incidence of pelvic recurrences. In a series of 58 consecutive patients between 1979 and 1980, those with primarily operable rectal tumours received preoperative irradiation in a total dose of 25.5 Gy in fractions of 5.1 Gy daily for 5 days. A locally 'curative' resection was performed in 41 patients 2 to 5 days later. The radiation therapy was well tolerated without early or late complications. All patients were followed up every 6 months with computed tomography over the pelvic region; aspiration biopsies were taken whenever any possible pathology was made. A local recurrence occurred in only 2 patients during the follow-up period (24-42 months). In a retrospective material from 1976 to 1979, totally 107 patients were admitted for surgery. Of 81 patients operated upon for 'local cure', 38 (47%) have had local recurrence to date, 32 (40%) of them within 2 years. These results indicate that a high-dose fractionated preoperative irradiation reduces the incidence of local recurrences, is well tolerated and does not influence the period of hospitalization.

Adenocarcinoma↗
PubMed1981

Analysis of developmentally homogeneous neural crest cell populations in vitro. IV. Cell proliferation and synthesis of glycosaminoglycans.

Glycosaminoglycans (GAG) have been implicated as regulators of morphogenesis and differentiation. We have cultured two different homogeneous populations of quail trunk neural crest cell with different predictable phenotypes after equivalent times in culture, and used these populations to distinguish changes in GAG synthesis before and after cell differentiation from changes that depend on time in culture, and that thus may reflect a non-specific response to in vitro culture conditions. Cells derived from the outgrowths surrounding explanted neural tubes remained undifferentiated. These crest cells showed a decrease in [3H]glucosamine incorporation into total GAG with time in culture. A similar decrease in total GAG, and, in addition, a slight decrease in the proportion of hyaluronic acid (HA) was observed in cultures of cluster-derived cells that homogeneously differentiated into melanocytes. Putative mouse neural crest cells that did not form melanin under the present culture conditions showed, similarly to the quail neural crest cells, a high incorporation of [3H]glucosamine into HA relative to sulfated GAG. The proportion of HA did not decrease with time in culture in these mouse crest cells. When pigment granules appeared in avian crest cells, the proliferation rate decreased drastically, whereas the proliferation rate of cells that did not form pigment granules remained constant. The results indicate that time in culture rather than either a differentiation per se or a change in the rate of proliferation, is largely responsible for the observed changes in GAG synthesis.

Animals↗
PubMed1981

Computed tomography of recurrent rectal carcinoma.

Computed tomography was performed in 28 patients and gave a correct diagnosis in 21 of 22 cases with abdomino-perineal excision of the rectum and in 5 of 6 with anterior resection. CT is of considerable value in the diagnosis of local recurrence of rectal carcinoma, particularly after rectal excision.

Aged↗