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Biomedical subjects

B Fekete

Publications and source records attributed to B Fekete.

At least 37 records · Page 2Linked to original sources
PubMed1986

Complex immunological monitoring of breast cancer patients treated postoperatively by electron beam irradiation.

To monitor the electron beam therapy some immunological parameters of breast cancer patients previously undergone surgery were tested before, during and after irradiation. Immune complex levels measured by complement consumption technique were not altered by irradiation. Killer cell activity tested in so-called antibody dependent cellular cytotoxicity (ADCC) capacity assay showed a marked decrease in some cases. Based on the phagocytic capacity of the granulocytes the patients could be divided into two groups: one with declining activity and another with rising activity: The majority of the patients (22/45) were humoral leukocyte adherence inhibition (H-LAI) negative before and during irradiation. Those showed positive H-LAI indices before electron therapy had unchanged (7/45) or decreasing (9/45) tendencies during the observation period. Further study is needed to establish the clinical relevance of these in vitro assays used by us.

Adult↗
PubMed1985

The humoral leukocyte adherence inhibition test in the investigation of lung cancer treated by surgery and radiotherapy.

17 operable and 13 inoperable lung cancer patients entered the study. In all patients, the humoral leukocyte adherence inhibition (H-LAI) test was positive before treatment. 7-20 days after surgery the H-LAI values decreased to normal levels in 8/8 cases. On radiotherapy, the same decrease was valid in 7/9 patients. Two exceptions in which no H-LAI reduction could be observed were in correlation with tumor progression.

Aged↗
PubMed1985

Humoral leukocyte adherence inhibition (H-LAI) with basic encephalitogenic protein (BEP) in head and neck cancer.

Fourty-five patients with head and neck cancer and 30 healthy subjects were evaluated by a modified humoral leukocyte adherence inhibition assay using basic encephalitogenic protein as antigen. Ninety-three percent of cancer patients gave a positive reaction, whereas every control person remained negative. The results suggest that humoral leukocyte adherence inhibition assay performed with basic encephalitogenic protein may be useful as an early diagnostic tool in head and neck cancer.

Adult↗
PubMed1985

Humoral leukocyte adherence inhibition (H-LAI) before and after surgery in human breast cancer.

Eighty-four breast cancer patients were tested by the H-LAI method before and after surgery. In the cancer groups positive H-LAI responses could be stated in 90--100%. In benign breast diseases 94% positivity was also detected. The H-LAI values of the cancer patients did not change significantly at their discharge from hospital and only insignificant reduction in the H-LAI indices were detected even 3--12 months after mastectomy. The benign cases showed, however, a definite declining tendency in H-LAI after surgery.

Adult↗
PubMed1983

Clinical correlates of circulating immune complex levels in advanced lung cancer. A discrimination analysis.

Sera from 53 patients with unresectable lung cancer were tested for the presence of immune complexes by 12 assays. 5 assays (EA rosette inhibition, ADCC inhibition, platelet aggregation, IgG and C3 concentrations in PEG precipitates) could discriminate cancer patients from healthy subjects with over 80% reliability. On the basis of 3 assays (EA-I, ADCC-I and PEG-C3) a function allowing a 100% correct classification could be formulated:--(EA-I)--0.5 (ADCC-I) + 2.4 (PEG-C3) greater than 69.3, i.e., results higher than 69.3 are characteristic for cancer patients and lower than 69.3 for normal subjects. The relationship between the immune complex levels and the average survival time was not altered by sex, age, histology and treatment. None of the immune complex assays or their combination were useful for the estimation of individual life expectation.

Adult↗
PubMed1983

The humoral leukocyte adherence inhibition in the discrimination among histologic types of lung cancer.

In an attempt to differentiate among histologic types of lung cancer, a modified humoral leukocyte adherence inhibition (H-LAI) assay was used. All types of lung cancer gave positive H-LAI indices against both homologous and autologous tumor extracts of the relevant histologic types. Positive H-LAI values were obtained even with unrelated tumor extracts. Cross-reactivity suggests that common antigen determinants or antigens of broad specificity play a role in the H-LAI assay.

Adenocarcinoma↗
PubMed1982

Investigation of circulating immune complexes in patients with breast cancer.

Circulating immune-complex (IC) levels of patients with breast cancer were measured before and after surgery using a complement-consumption technique. 16 of 26 patients showed raised pre-operative IC values. In 15 out of 16 patients the pathological IC levels decreased within 2--10 days following surgery. Suggestions about the applicability of this test not only for prognosis, but also for monitoring the course of breast cancer need to be confirmed by further investigations.

Adult↗
PubMed1982

Demonstration of Fc and C3b receptors on rat perikarya.

Minced rat brain deprived of cerebellum was dissociated by trituration through stainless steel screen and nylon meshes, then by velocity sedimentation technique free-floating perikarya were separated from cell syncytia and cellular debris. The presence of Fc-receptor and C3b-receptor activities, as well as the absence of membrane-bound immunoglobulin and receptor for sheep blood cells were demonstrated on separated perikarya of the rat brain.

Animals↗
PubMed1982

Immunological monitoring in lung cancer: use of the leukocyte migration inhibition assay.

The agarose microdroplet leukocyte migration inhibition assay has been applied to the management of patients with lung cancer. Sera of patients with various stages of the disease were assayed in the indirect LMI system for their influence on the LIF production of normal leukocytes. A probably cell-mediated immunity blocking activity could be detected both in the serum of patients with inoperable tumours and in that of operable patients before and two weeks after surgery. The activity, however, disappeared in two thirds of the cases in about 6 months after surgery. The immune competence of patients with inoperable tumours was investigated before and during combined chemotherapy. A positive correlation was established between the tuberculin skin reactions and the leukocyte migration inhibition obtained in the direct LMI assay against PPD, but only in the presence of autologous serum. Sera of patients were also tested for putative in vivo LIF activity by their effect on the migration of autologous leukocytes. A significant correlation between anergy to PHA, as evaluated by skin testing, and the migration inhibition caused by sera was found. When, however, sera from healthy persons and from patients with chronic bronchitis or lung cancer were compared for their effect on the migration of normal leukocytes, no difference could be detected among the three groups. Single doses of the drugs temporarily weakened the skin reactions to PHA and the migration inhibition to PPD in autologous serum, but activity was restored when the drug influence had ceased.

Binding, Competitive↗
PubMed1982

A simple method to evaluate leucocyte adherence and adherence inhibition in tubes.

A simple rapid method for the measurement of leucocyte adherence and adherence inhibition in tubes is described. The procedure is based on determining the amount of cell protein. We compared measurement of adherent cells by protein assay with the automated counting of the non-adherent cells in the tube leucocyte adherence inhibition test. Both procedures gave quantitatively similar results and a high statistical correlation was found between them. The results suggest that the protein assay is a reliable substitute for cell counting.

Antigens, Neoplasm↗
PubMed1981

Evaluation of different methods for detecting circulating immune complexes. Studies in patients with lung cancer.

In a collaborative study involving 7 laboratories, sera from 53 patients with lung cancer, 37 primary and 16 secondary tumours, and sera of 40 healthy blood donors were tested by 19 different assays or assay modifications used for detecting immune complexes. In 12 out of 19 assays, significantly higher immune complex levels were found in the cancer patients than in the healthy subjects. Assays based on interactions between immune complexes and Fc receptors of different cells (lymphocytes, macrophages of platelets) discriminated between cancer patients and health subjects and a high percentage (47-87%) of positivity was observed in such assays in patients with lung cancer. In contrast, none of the tests based on immune complex-complement interactions discriminated between cancer patients and health subjects. Immunochemical analyses of the PEG precipitates obtained from the sera tested revealed that the concentrations of IgG, IgA and C3 were significantly higher in the precipitates obtained from patients sera than from control sera, but no significant differences were seen in IgM and C1q concentrations. A 100% correct classification of individuals tested was obtained on discriminant analysis of results with 3 assays: EA rosette inhibition, ADCC inhibition and C3 concentration in PEG precipitates. Correlation between results obtained with individual sera by the different assays was very poor: significant correlation coefficients were found in only 13% of all possible paired comparisons. Our results suggest that Fc receptor-dependent assays are more suitable for detection and measurement of circulating immune complexes in lung cancer than tests based on interactions with complement.

Adenocarcinoma↗
PubMed1981

Short-term effect of irradiation on the peripheral lymphocyte populations in patients treated with high-energy photon beam.

Changes in lymphocyte populations wee studied in 22 patients with testicular cancer before and after a single 2-Hy dose of high-energy photon beam. Absolute lymphocyte counts as sell as total T-and active T cells markedly decreased immediately after irradiation, whereas no significant alterations in Fc receptor-bearing lymphocytes and B cell counts were produced. Recovery of lymphocyte populations was relatively rapid, reaching pre-irradiation values by 24 hours.

Adolescent↗
PubMed1981

Investigation of immunoglobulins, circulating immune complexes and plasma free hemoglobin in cancer patients on 60Co gamma-ray therapy.

32 patients with different tumours were irradiated by 60Co gamma-rays. During therapy lasting for several weeks, changes in the content of immunoglobulin and of some other serum proteins, circulating immune complexes and plasma free hemoglobin were determined. Immunosuppression according to immunoglobulin content in serum was not produced by this type of radiation. Decrease in immune complex levels was a good prognostic sign. Low values of plasma hemoglobin content during treatment indicated that no erythrocyte membrane damage had been effected.

Adolescent↗