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Biomedical subjects

B Falini

Publications and source records attributed to B Falini.

At least 199 records · Page 11Linked to original sources
PubMed1981

Detection of terminal transferase in paraffin sections with the immunoperoxidase technique.

Terminal deoxynucleotidyl transferase (TdT) is an early T-cell differentiation marker in a number of species, including man. We have demonstrated TdT in the nuclei of cortical thymocytes in paraffin-embedded sections of calf, rat, and human thymus by indirect immunoperoxidase techniques. In addition, these techniques have been used to verify and extend enzyme assay results by detecting TdT in blast cells from 10 patients with convoluted T-cell lymphoma/leukemia, 1 patient with acute granulocytic leukemia, 1 patient with chronic granulocytic leukemia in blast crisis, and 1 patient with non-T non-B acute lymphocytic leukemia.

Animals↗
PubMed1980

Erythrophagocytosis by undifferentiated lung carcinoma cells.

A case is reported of undifferentiated metastatic lung carcinoma in which both a supraclavicular lymph node and bone marrow were infiltrated by anaplastic tumor cells which had engulfed erythrocytes and nucleated elements. The possibility of a histiocytic origin for the phagocytizing neoplastic cells was excluded by ultrastructural, cytochemical, and immunoperoxidase studies. This investigation shows that epithelial tumor cells may, at times develop phagocytizing activity, a phenomenon observed in certain experimentally induced tumors. The mechanism responsible for this phenomenon is not clear.

Bone Marrow↗
PubMed1980

Protein A-peroxidase conjugates for two-stage immunoenzyme staining of intracellular antigens in paraffin-embedded tissues.

Staphylococcal protein A conjugated to horseradish peroxidase was employed in an indirect immuno-staining technique to identify intracellular antigens in paraffin-embedded tissues. The sections were incubated with specific antisera and the antigen-IgG complexes demonstrated with protein A-peroxidase conjugate. Immunoglobulins, lysozyme and insulin were satisfactorily detected by this technique. A comparison of this method with the PAP, "labelled antigen" and peroxidase-labelled antibody sandwich techniques was made.

Antigens↗
PubMed1980

Topographical localization of intracellular immunoglobulins in hairy cells by immunoelectron microscopy.

Ultrastructural characterization and localization of intracellular immunoglobulin in peripheral blood mononuclear cells from a patient with hairy cell leukemia were studied by the direct immunoperoxidase technique. Approximately 20--30% of hairy cells stained positive for gamma-heavy and K-light chains, and this staining was limited to certain rough endoplasmic reticulum profiles. These ultrastructural findings can be considered an additional argument for the B cell origin of hairy cells.

Endoplasmic Reticulum↗
PubMed1979

[Cellular immunity in patients with Hodgkin's disease: modification induced by incubating lymphocytes with fetal calf serum and Hodgkin's serum].

The capacity of peripheral blood lymphocytes from fifteen patients with untreated Hodgkin's disease to form E-rosettes with sheep erytrocytes and to respond to PHA stimulation was found to be impaired in 47% of patients. When peripheral blood lymphocytes were incubated overnight in culture medium containing 20% fetal calf serum, E-RFC levels and the capacity to respond to PHA returned to normal. These functions were resuppressed by additional incubation with Hodgkin's disease serum but not from normal serum. The Hodgkin's disease serum inhibited normal peripheral blood lymphocytes to form E-rosettes and to respond to PHA.

Animals↗
PubMed1978

[Rosette formation test with mouse erythrocytes in chronic lymphatic leukosis: correlation with other membrane receptors].

The lymphocyte subpopulations of CLL patients have been studied. IgM and IgD membrane-associated immunoglobulins alone or in combination have been found, IgG being present in only one case. A marked increase in mouse-complement receptors (as shown by the EACt rosettes) as compared with human complement receptors (as shown by the EACa rosettes) was observed. The population of SIg-bearing lymphocytes was significantly greater than that of complement bearing lymphocytes. Moreover a mean percentage of 60.7 +/- 17 of the leukaemic lymphocytes formed rosettes with mouse red blood cells. In a significant number of patients the E-rosettes forming cells were also increased.

Erythrocytes↗
PubMedDate not supplied

Vaccine therapy of B cell malignancies: different strategies for a novel approach.

This review deals with the theoretical principles and experimental results of immunotherapy for B cell malignancies, namely for non-Hodgkin lymphomas (NHLs) and multiple myeloma. Its focus is the use of vaccines in clinical practice with particular emphasis on the most recent developments and therapeutic opportunities arising from combination therapies. Previous studies will be reviewed and the present status of vaccine technology summarized.

Animals↗
PubMedDate not supplied

Hodgkin's disease: update of findings.

The authors critically review the problem of Hodgkin's disease (HD) in the light of new morphological, immunohistochemical, kinetic, genotypic, and virological findings. These support the lymphoid origin of neoplastic Hodgkin's and Reed-Sternberg cells, because of regular expression of the CD30 lymphoid activation antigen and frequent detection of B- or T-cell phenotypic and/or genotypic markers. It is possible to hypothesize the release of cytokines by tumoral elements as well as the presence of specific cytokine receptors on their surface. This might explain some clinical and pathological features, such as fever, loss of weight, eosinophilia and attraction of reactive elements that make up the composite cellular milieu of typical HD. Integration of monoclonal EBV in the genoma of neoplastic elements has repeatedly been shown, and this might play an essential role in the pathogenesis of the disease. On the basis of present concepts, the borderlines between HD and some categories of non-Hodgkin's lymphomas--especially the anaplastic large cell forms--have become somewhat blurred. Additional research work in the field of HD is desirable and might pave the way for new and more effective therapeutic approaches, designed on the basis of the natural history of the neoplasm.

Antigens, CD↗
PubMedDate not supplied

Immunohistochemistry of malignant lymphomas. Advantages and limitations of the new monoclonal antibodies working in paraffin sections.

On the basis of the experience derived from the study of 2,000 biopsies, the authors critically review the problem of the newly generated monoclonal antibodies (mAbs) working in paraffin sections. The reliability and drawbacks of the most commonly used reagents are examined, with special reference to their employment in the diagnosis of malignant lymphomas. The authors underline that only a few mAbs are really specific, since most reagents give rise to a certain number of cross-reactivities. In the light of this, a wide panel of antibodies should always be applied in the diagnosis of lymphoid tumours in order to avoid possible misinterpretations, which can actually lead to wrong therapeutical decisions.

Antibodies, Monoclonal↗
PubMedDate not supplied

[Peripheral T-cell lymphoma associated with hemophagocytic syndrome: a recently identified entity. Clinico-pathologic and immunohistochemical study of 2 cases].

The authors report on two examples of peripheral T-cell lymphoma associated with hemophagocytic syndrome. The latter represents a recently described entity, which is characterized by pancytopenia, hepatosplenomegaly, fever and rapid downhill clinical course. Microscopic examination of different tissue samples shows bone-marrow, liver and spleen infiltration by small-medium sized lymphoid elements, which display peripheral T-cell phenotype and are associated with an exuberant hyperplasia of hemophagocytosing, benign-looking histiocytes. The clinical and pathological findings, along with the main differential diagnoses, are discussed.

Erythrocytes↗