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Biomedical subjects

B D Smith

Publications and source records attributed to B D Smith.

157 records · Page 9Linked to original sources
PubMedDate not supplied

Lateralized cortical/cognitive processing and bilateral electrodermal activity: effects of sensory mode and sex.

Forty-eight subjects were assigned to four equal-size cells defined by sex and familial handedness (presence versus absence of an immediate family member who is left-handed or ambidextrous). Subjects were presented a series of 40 stimuli, each of which was designed to represent the intersection of a sensory mode (visual versus auditory) and hemisphere mode (verbal versus spatial). The dependent measures were the number of stimulus-specific and nonspecific responses with magnitude larger on the lefthand. On the whole, results are supportive of the contralateral inhibitory process hypothesized by LaCroix and Comper (1979). Nonspecific response data appear to be sensitive to hemispheric manipulation as observed previously in this laboratory. Sensory mode appears to be an important determinant of lateralized phasic magnitude differences, with males showing the most striking verbal-spatial distinctions during visual mode presentation.

Auditory Perception↗
PubMedDate not supplied

Pharmacy staff interventions in a medical center hematology-oncology service.

OBJECTIVES: Many pharmaceutical care efforts remain undocumented, resulting in underestimation of the importance of the pharmacy staff interventions and missed opportunities to find new directions for quality improvement. The purpose of this project was to document and analyze the pharmaceutical care interventions of the staff of a hematology-oncology pharmacy. DESIGN: Interventions were self-reported by pharmacy staff members. The data collection period was October 1, 1995, to May 31, 1996. Intervention analysis consisted to types of interventions performed, categories of personnel performing interventions, intervention acceptance rate by staff physicians, and medication cost avoidance. PARTICIPANTS: This project was performed by the Hematology-Oncology Pharmacy Service, Department of Pharmacy, Walter Reed Army Medical Center, Washington, D.C. SETTING: Walter Reed Army Medical Center, Washington, DC, a 1,000-bed teaching and research institution of the U.S. Army Medical Department. RESULTS: Pharmacy staff reported 503 interventions. The leading categories of interventions were clinical consultations (167), correction of prescribing errors (85), and patient treatment procedures (65). The interventions were primarily initiated by oncology pharmacists and residents (68.8%) and oncology pharmacy technicians (30.6%). The intervention acceptance rate was 97%. Medication cost avoidance was $23,091. CONCLUSION: A significant amount of pharmaceutical care was documented by the hematology-oncology pharmacy staff, with both oncology pharmacists and oncology pharmacy technicians making key contributions. The pharmacy staff's interventions had a high rate of acceptance by the medical and nursing staffs, and resulted in significant medication cost avoidance.

District of Columbia↗
PubMedDate not supplied

High-generation polycationic dendrimers are unusually effective at disrupting anionic vesicles: membrane bending model.

The membrane disruption properties of high generation (G4 to G7) poly(amidoamine) (PAMAM) dendrimers are evaluated and compared to linear poly(lysine). The G6 and G7 dendrimers are unusually effective at inducing leaky fusion of anionic, large unilamellar vesicles, as determined by standard fluorescence assays for lipid mixing, leakage, and contents mixing. Both G7 dendrimer and poly(lysine) are able to disrupt sterically stabilized vesicles that are coated with poly(ethylene glycol). A G7 dendrimer/DNA complex with a 1:1 concentration ratio of dendrimer surface amines to DNA phosphate groups is unable to induce leakage of 3:7 POPA-PE vesicles; however, extensive leakage is observed when the surface amine to phosphate stoichiometry is >/=3:1. Thus, the DNA/dendrimer complexes that typically induce high levels of cell transfection are also able to induce high levels of vesicle leakage. The G7 dendrimer does not induce membrane phase separation in 3:7 POPA-PE vesicles, but an inverse hexagonal phase is observed by (31)P NMR. The enhanced membrane disruption is interpreted in terms of a membrane bending model. A rigid, polycationic dendrimer sphere uses electrostatic forces to bend a malleable, anionic membrane and induce bilayer packing stresses. This bending model is biomimetic in the sense that protein-induced membrane bending is currently thought to be an important factor in the fusion mechanism of influenza virus.

Anions↗
PubMedDate not supplied

Synthesis and characterization of NVOC-DOPE, a caged photoactivatable derivative of dioleoylphosphatidylethanolamine.

A caged, photocleavable derivative of dioleoylphosphatidylethanolamine (DOPE) called NVOC-DOPE was prepared by reaction of DOPE with 6-nitroveratryloxycarbonyl chloride. In contrast to egg phosphatidylethanolamine (EPE), NVOC-DOPE or its 1:1 mixture with EPE forms liposomes at both pH 7.4 and 5.0. Photolysis (lambda > 300 nm) of aqueous liposomal dispersions of NVOC-DOPE at pH 9.0, 7.4, or 5.0 results in complete conversion to DOPE and subsequent release of entrapped calcein dye. The temporal and spatial control associated with the photorelease technique suggests that NVOC-DOPE can be used to study a range of important dynamic membrane processes such as membrane fusion and the action of membrane-associated enzymes.

Benzaldehydes↗
PubMedDate not supplied

Glycolytic enzyme binding during entrance to daily torpor in deer mice (Peromyscus maniculatus).

Associations of glycolytic enzymes with the subcellular particulate fraction of skeletal muscle and heart were examined during entrance to daily torpor in deer mice (Peromyscus maniculatus). In skeletal muscle a significant decrease in enzyme binding occurred during torpor entrance for phosphofructokinase, glyceraldehyde-3-phosphate dehydrogenase, and pyruvate kinase, with an additional significant decrease for phosphofructokinase and pyruvate kinase during the deepest state of torpor. Reductions in enzyme binding during torpor entrance also occurred in heart; significant changes were observed in hexokinase, glyceraldehyde-3-phosphate dehydrogenase, and pyruvate kinase binding. Contrary to the finding of additional reductions in enzyme binding seen in skeletal muscle, significant increases in enzyme binding during the deepest torpor state were observed for hexokinase, phosphofructokinase, glyceraldehyde-3-phosphate dehydrogenase, pyruvate kinase, and lactate dehydrogenase in heart. These results suggest that a decrease in the binding of glycolytic enzymes to subcellular structures in skeletal muscle and heart may be at least partially responsible for initiating the reduction in metabolic rate during torpor entrance. This decreased binding may continue to mediate the metabolic reduction in skeletal muscle throughout torpor; heart, however, may require the use of different molecular mechanisms. The increased binding in heart during the deepest state of torpor may represent an anticipatory response in preparation for increased activity during arousal.

Analysis of Variance↗
PubMedDate not supplied

Congenital bronchoesophageal fistula in the adult.

Congenital bronchoesophageal fistulas usually present in infants and may occur with or without esophageal atresia. Twenty cases of congenital bronchoesophageal fistula in adults have been reported in the English literature. Another case of this rare developmental anomaly is presented here. The radiographic, endoscopic, surgical, and pathologic findings are discussed with a review of the literature.

Aged↗
PubMedDate not supplied

Association of antidepressants and other medications with mortality in the residential-care elderly.

To explore the extent to which treatment of depression affects survival, we evaluated the association between use of antidepressant medications and death rates among the residents of a large residential-care facility for the elderly using a retrospective record-review study (N = 624). One year survival, among those taking antidepressants (10.9%), was 11.8% compared to 11.1% among the remainder of the population. A second study followed a group of 32 patients in the same institution who had participated in a therapeutic trial of nortriptyline treatment for major depression. Patients who experienced adverse medical events during treatment exhibited significantly increased mortality; among treatment completers, there was no significant relationship between mortality and therapeutic response. These findings suggest that the inability to tolerate treatment with an antidepressant can be considered a manifestation of physiologic frailty and increased vulnerability to mortality from disease. The previously reported decrease in survival among residential-care patients with major depression is not paralleled by a similar effect in those taking antidepressants. This may reflect selection factors with respect to the ability to tolerate antidepressants, rather an effect of treatment.

Aged↗
PubMedDate not supplied

Affect cognizance and the type A behavior pattern: labeling, cognitive effort or phenomenological loss?

Forty-eight subjects were requested to remember three idiosyncratic emotion-laden memories (eliciting Anger, Anxiety and Contentment). Objective (change in tonic skin conductance) and subjective (rated success at achieving each emotion) measures of arousal were taken for each condition as well as subjectively rated use of cognitive content (verbal and visuospatial modes). An "Affect Cognizance Index" (group-calculated T-score for objective arousal minus group-calculated T-score for subjective arousal) correlated positively with a measure of Type A Behavior during Anxiety and Contentment but not Anger, with or without cognitive effort statistically controlled. However, cognitive content may influence affect cognizance independent of its relationship with the Type A syndrome. Those subjects reporting greater verbal content showed augmented experience of affect-induced arousal during Anger. Visuospatial content augmented subjective experience of affect-induced arousal during Contentment.

Adaptation, Psychological↗
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New concepts in the treatment of acute myeloid malignancies: selected pathways for targeted therapy.

Acute myeloid leukemia (AML) and myelodysplasia (MDS) continue to present formidable treatment challenges as there is not yet a "standard approach" that reliably and safely cures the majority of adults with these disorders. Understanding the basic cellular and molecular biology of leukemia is vital to the development of better treatment approaches. All malignant cells, including leukemias, carry survival advantages compared to their normal counterparts. These survival advantages are particularly evident when the cells face both normal environmental stressors as well as extreme stressors of potentially lethal DNA damage. The impact of such stressors is ultimately determined by the signaling pathways and resultant cellular activation, including alterations in cellular growth, differentiation, survival and death. It is our improving understanding of these signaling pathways, both normal and malignant, that provide the targets for novel therapies. In this review article, we will focus on several signaling pathways, as well as the regulation of the cell cycle, that not only represent potentially accessible sites for intervention in myeloid malignancies, but those that are actively being studied.

Antibodies, Monoclonal↗