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Biomedical subjects

B D Smith

Publications and source records attributed to B D Smith.

At least 145 records · Page 8Linked to original sources

Identification of the collagenous proteins synthesized by cultured cells from human skin.

Cells cultured from human skin synthesize precursor forms of types I and III collagens. After denaturation and reduction, the polypeptide chains obtained from these molecules had molecular weights estimated to be 140,000 and 120, 000. The larger chains, the pro alpha chains, are believed to be derived from the original precursor molecules. The smaller chains arise from altered forms, p-collagens, that may be normal intermediates in the conversion of both procollagens to collagens I and III.

Animals↗

Production of procollagen by human fibroblasts in culture.

Three hydroxyproline-containing proteins secreted into the medium by human fibroblasts in culture were isolated and characterized. A minor fraction was identical to the collagen monomer. The major fraction was a form of procollagen, which contained, in addition to pro alpha and alpha chains, a component estimated to have a molecular weight of 250,000. This component was a dimer of pro alpha chains joined by disulfide bonds. The third fraction, much lower in hydroxyproline and hydroxylysine content, was of still greater size. Pro alpha chains were released upon denaturation and reduction, indicating that this fraction may contain pro alpha chains linked by disulfide bonds to noncollagenous material.

Carbon Isotopes↗

Laparoscopy.

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Adolescent↗

Culdoscopy.

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Culdoscopy↗